KCC2 (SLC12A5) (NM_020708) Human Untagged Clone

CAT#: SC304801

SLC12A5 (untagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2



  "NM_020708" in other vectors (6)

CNY 7,776.00


货期*
现货

规格
    • 10 ug

Cited in 4 publications.

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


SLC12A5 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DEE34; EIEE34; EIG14; hKCC2; KCC2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_020708 edited
GGGCGGCCGCGAATTCGCCCTTAGAGCTACAGCGAACGAGAGAGCGGCGAAGGCGGGTAG
AGGGGCGCGGGCGAGGCGGCGCAGCCATCCCCGGACCAGGGGCCGCGCCGCCACCATGCT
AAACAACCTGACGGACTGCGAGGACGGCGATGGGGGAGCCAACCCGGGTGATGGCAACCC
CAAGGAAAGCAGTCCCTTCATCAACAGCACCGACACAGAGAAGGGAAAGGAGTATGATGG
CAAGAACATGGCCTTGTTTGAGGAGGAGATGGACACCAGCCCTATGGTGTCCTCCTTGCT
CAGTGGCCTGGCCAACTACACCAACCTGCCCCAGGGAAGTAGGGAGCATGAAGAGGCAGA
AAACAATGAGGGTGGAAAAAAGAAGCCGGTGCAGGCCCCACGCATGGGCACCTTCATGGG
CGTGTACCTGCCGTGCCTGCAGAACATCTTTGGCGTCATCCTCTTCCTGCGGCTCACCTG
GGTGGTGGGCATTGCAGGCATCATGGAGTCCTTCTGCATGGTGTTCATCTGCTGCTCCTG
TACGATGCTCACGGCCATCTCCATGAGTGCAATTGCAACGAATGGTGTTGTGCCTGCTGG
TGGCTCCTACTACATGATTTCCAGGTCTCTGGGCCCAGAGTTTGGGGGTGCCGTGGGCCT
CTGCTTCTACCTGGGCACTACCTTTGCAGGAGCCATGTACATCCTGGGCACCATCGAAAT
CCTGCTGGCTTACCTCTTCCCAGCCATGGCCATCTTCAAGGCAGAAGATGCCAGTGGGGA
GGCAGCAGCCATGCTGAACAACATGCGTGTTTACGGCACCTGTGTGCTCACCTGCATGGC
CACTGTGGTGTTTGTGGGTGTCAAGTATGTCAACAAGTTTGCCCTTGTCTTCCTGGGTTG
TGTCATCCTCTCCATCCTGGCCATCTATGCTGGGGTCATCAAGTCTGCCTTCGACCCACC
CAACTTCCCGATCTGCCTCCTGGGTAACCGCACGCTGTCTCGCCATGGCTTTGATGTCTG
TGCCAAGCTGGCTTGGGAAGGAAATGAGACGGTGACCACACGGCTATGGGGCCTTTTCTG
CTCCTCTCGCTTCCTCAACGCCACCTGTGATGAATACTTCACCCGAAACAATGTCACAGA
GATCCAGGGCATCCCTGGTGCTGCCAGTGGCCTCATCAAAGAGAACCTCTGGAGCTCCTA
CCTGACCAAGGGCGTGATTGTGGAGAGGAGTGGGATGACCTCGGTGGGCCTGGCCGATGG
CACTCCTATCGACATGGACCACCCTTATGTCTTCAGTGATATGACCTCCTACTTCACCCT
GCTGGTTGGCATCTACTTCCCCTCAGTCACAGGGATCATGGCTGGTTCTAACCGCTCTGG
GGACCTGAGGGATGCCCAGAAGTCAATCCCCACTGGCACCATCCTGGCCATCGCCACCAC
CTCTGCTGTCTACATCAGCTCCGTTGTTCTGTTTGGGGCCTGCATTGAGGGGGTCGTCCT
GCGGGACAAGTTTGGCGAAGCTGTGAATGGCAACCTCGTGGTGGGCACTCTGGCCTGGCC
ATCTCCATGGGTAATTGTCATCGGATCCTTCTTCTCCACCTGTGGGGCTGGGCTGCAGAG
CCTCACGGGGGCCCCACGCCTGCTGCAGGCCATCTCGAGGGATGGCATTGTGCCCTTCCT
GCAGGTCTTTGGCCATGGCAAGGCCAATGGAGAGCCGACCTGGGCCCTGCTCCTGACTGC
CTGCATCTGCGAGATTGGCATCCTCATTGCATCCCTCGACGAGGTGGCCCCCATCCTCTC
TATGTTCTTCCTGATGTGCTACATGTTTGTGAATCTGGCCTGTGCAGTGCAGACGCTGCT
GAGGACACCCAACTGGAGGCCACGCTTTCGATATTACCACTGGACCCTCTCCTTCCTGGG
CATGAGCCTCTGCCTGGCCCTCATGTTCATCTGCTCCTGGTATTATGCACTGGTAGCCAT
GCTCATTGCTGGACTCATCTACAAGTACATTGAGTACCGTGGGGCAGAGAAGGAGTGGGG
CGATGGGATACGAGGTCTGTCTCTCAGTGCGGCTCGCTATGCCCTCTTACGCCTGGAGGA
AGGGCCCCCACACACCAAGAACTGGAGGCCACAGCTGCTGGTGCTGGTGCGTGTGGACCA
AGACCAGAATGTGGTGCACCCCCAGCTGCTCTCACTGACCTCCCAGCTGAAGGCAGGGAA
GGGCCTGACCATCGTGGGCTCTGTCCTTGAGGGCACCTTTCTGGAAAATCATCCACAGGC
CCAGCGGGCAGAAGAGTCTATCAGGCGCCTGATGGAGGCAGAGAAGGTGAAGGGCTTCTG
CCAGGTGGTGATCTCCTCCAACTTGCGTGATGGCGTGTCCCATCTGATCCAGTCCGGGGG
CCTCGGGGGGCTGCAGCACAACACTGTGCTTGTTGGCTGGCCCCGCAACTGGCGCCAGAA
GGAAGATCATCAGACATGGAGGAACTTCATTGAGCTGGTCCGGGAAACCACAGCTGGCCA
CTTAGCCCTGCTGGTCACCAAGAACGTTTCCATGTTTCCTGGGAACCCTGAGCGCTTCTC
TGAGGGCAGCATCGACGTTTGGTGGATTGTGCACGATGGAGGCATGCTCATGCTGCTGCC
CTTCCTGCTGCGGCACCACAAGGTCTGGCGGAAGTGCAAGATGCGTATCTTCACTGTGGC
CCAGATGGATGACAATAGCATCCAGATGAAGAAGGATCTGACCACATTTCTGTATCATTT
ACGCATCACTGCGGAGGTCGAGGTGGTGGAGATGCATGAGAGCGACATCTCAGCTTACAC
CTATGAGAAGACGTTGGTGATGGAGCAGCGTTCCCAGATCCTCAAACAGATGCATTTAAC
CAAGAATGAGCGGGAGCGGGAGATCCAGAGTATCACAGATGAGTCACGAGGCTCAATCCG
GAGAAAGAATCCAGCCAACACGCGGCTCCGCCTGAACGTCCCAGAAGAGACGGCTGGTGA
CAGTGAAGAGAAGCCAGAGGAGGAGGTGCAGCTGATCCACGATCAGAGTGCTCCCAGCTG
CCCCAGCAGCTCCCCGTCCCCAGGGGAGGAGCCTGAGGGGGAAGGGGAGACAGATCCGGA
GAAGGTGCATCTCACCTGGACCAAGGACAAGTCGGTGGCAGAGAAGAATAAGGGCCCCAG
TCCTGTCTCCTCTGAGGGCATCAAGGACTTCTTCAGCATGAAGCCGGAGTGGGAGAACTT
GAACCAGTCCAACGTGCGGCGCATGCACACGGCCGTGCGGCTGAACGAGGTCATCGTGAA
GAAATCCCGGGACGCCAAGCTTGTTTTGCTCAACATGCCTGGGCCTCCCCGCAACCGCAA
TGGTGATGAAAACTACATGGAGTTTCTCGAGGTCCTCACAGAGCACCTGGACCGGGTGAT
GCTGGTCCGCGGCGGCGGCCGCGAGGTCATCACCATCTACTCCTGAGAACCAGGACCTGC
CACCCGGGCCCGAGCGCGCCCGGCCCGCGGCTCCGGAGCCCTCGCCGCGCCCCCCGCCGC
TGTCACCGTTTACATACAGACCCTGTGCCCGTGTCCTGGCCCCTTACCCCGCTGCCTGAA
GCCCGGAGGCCACGCCTGTTGGGGCTGATTCGGAGAGGGCGCCCCGCCGCGCAGAGACCA
GAGCTCCTCAGTGCCAGTTTGGCCCCTGGGTCTTCGCTGCCCTTTTTCTAAGCCCGGCCT
CGTCTCGCCGGAGGAGACGCTGCAATAAAGGTTGGGAGAAGGCGCGGAAAGGAGAGGAGC
TGGGGCCTTGGGGACCCCCAGGTAGTCCATGCGGCCCATTCCTCCCCTTCCCACTCCCGC
CGCGGTCCTCGCTCTGCGCTCCTCCGGCGCTGCTCCCTGGCTCCCGGCGGCCCGGAGGCC
CGCGGGGTGGGAAGGCCGCGCTTGCCGTCTCCGCCGCCCCTTCTCGCCGAGCCGTGGGGC
GCGGGCGGCCGAGCCTATACATAGTGTACAGGAGACATCGCGTGTATTTTTAACGTCCCC
ATATTTATGTGACTAGAAGCGCAACAGACTTCTCG
>OriGene 5' read for NM_020708 unedited
NGGCGTCAAAATTTGTATACGACTCACTATAGGGCGGCCGCGATTCGCCCTTAGAGCTAC
AGCGAACGAGAGAGCGGCGAAGGCGGGTAGAGGGGCGCGGGCGAGGCGGCGCAGCCATCC
CCGGACAGGGGCCGCGCCGCCACCATGCTAAACAACCTGACGGACTGCGAGGACGGCGAT
GGGGGAGCCAACCCGGGTGATGGCAACCCCAAGGAAAGCAGTCCCTTCATCAACAGCACC
GACACAGAGAAGGGAAAGGAGTATGATGGCAAGAACATGGCCTTGTTTGAGGAGGAGATG
GACACCAGCCCTATGGTGTCCTCCTTGCTCAGTGGCCTGGCCAACTACACCAACCTGCCC
CAGGGAAGTAGGGAGCATGAAGAGGCAGAAAACAATGAGGGTGGAAAAAAGAAGCCGGTG
CAGGCCCCACGCATGGGCACCTTCATGGGCGTGTACCTGCCGTGCCTGCAGAACATCTTT
GGCGTCATCCTCTTCCTGCGGCTCACCTGGGTGGTGGGCATTGCAGGCATCATGGAGTCC
TTCTGCATGGTGTTCATCTGCTGCTCCTGTACGATGCTCACGGCCATCTCCATGAGTGCA
ATTGCAACGAATGGTGTTGTGCCTGCTGGTGGCTCCTACTACATGATTTCCAGGTCTCTG
GGCCCAGAGTTTGGGGGTGCCGTGNGCCTCTGCTTCTACCTGGGCACTACCTTTGCAGGA
GCCATGTACATCCTGGGCACCATCGAAATCCTGCTGGCTTACCTCTTCCCAGCCATGGCC
ATCTTCAAGGCAAAGATGCCAGTGGGGAGGCCACACCCATGCTGAACACATGCGTGTTTA
CGGCACCTGTGTGCTCACCTGCATGGCCACTGGGGG
>OriGene 3' read for NM_020708 unedited
NCATTGGNGATGGCACTTCCAGGNCCAGNATAGCACTGGGGNAGGGTCACAGGGNAGCCA
CCCGGGATCTGTTCAGGAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTT
TTTTTTTTTTTAAGGGTATAATAAAATTATTTTTATTGCATTTTGTGCAGACGGGAGTCT
AAAAAAGTAATACAGTAAAAGCCATAAAGCATTAAATGGGAGCATTCAACAATAAAGGAA
AGAACCAACCCTCTCCTTTTCATATTGGCACAAAGAACACAAGATCAATACTGAGGCAGG
ATGCGGCATACTGAACACTTACACTGAAAATTAAAGGCAGGCCTAATAATAATAGCAGTA
ATAGTAATAAATACTCTTGATTTGGTTTGCAACCTCTCCTATTTACAAGGGTTTTCAACT
TCATTGCATTGCCCTGCACATAGATCATCTAAAACAAAAGCAACACTAGTTATCACTACA
AGGCTATTGAAATATTGCACTCAGAATAAAAGTCACTTGAATGGACATGAAACATCTAAC
TACAGTGATTATAAAGATAACTTGATGAATGACTTGTGATAACATTCATAACAAATATTG
CACATGTAGCACAGAAAATTTGGGTTTTTGTTGTGGGATGTCTGTTTCATCAACGGAAAC
TTTGCTCTTGCCAACCAGACAATCTGGGCCCAGATAGTGCAACTTTGGGTCCCTAATTGA
TGTTGATACAGCCCCCTTTCTGCACAGCCCATCACATCAGCTCTGGAATATTCAGGCCCC
CANAAAGGCAGACCTGGCTGTGGTGTTCACAAGGAGGGCTACTGAAAGATGCAGGGGCCT
GGTGTCTTAACACCTCCAGCTCCCTTGTTGAGTACTTGAAAGAATCTATCATGCCCC
Restriction Sites Please inquire     
ACCN NM_020708
Insert Size 3351 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020708.3, NP_065759.1
RefSeq Size 6059 bp
RefSeq ORF 3351 bp
Locus ID 57468
UniProt ID Q9H2X9
Protein Families Transmembrane
Gene Summary K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]
Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2, also known as KCC2b) has a distinct N-terminus and is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

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