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DET1 (NM_017996) Human Untagged Clone
CAT＃: SC304552
DET1 (untagged)-Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304552 representing NM_017996.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGGGACAGATACTAAAAAGAGATGTGATAATGGATCATCATGTTTCTACCATCAAGCCTCGAAGA ATCCAAAACCAAAATGTCATTCACCGCTTGGAACGCCGGCGGATCAGTTCAGGCAAGGCAGGTACCCAC TGGCACCAAGTCCGAGTGTTCCATCAGAATGTCTTCCCCAACTTCACAGTTGTCAACGTTGAAAAGCCT CCTTGTTTCTTGCGTAAATTCTCACCTGATGGACGCTACTTTATTGCTTTTTCTTCAGACCAGACATCT CTTGAAATCTATGAGTACCAGGGCTGCCAGGCAGCAGAGGACCTACTGCAGGGATACGAAGGAGAAATC CTGTCCAATGGCAATGACCAGCGGTCAGTGAATATCCGGGGCCGGCTCTTTGAACGCTTTTTTGTCCTG CTGCACATTACCAATGTTGCGGCCAATGGTGAGCACCTGAACCGGGAGTGTAGTCTCTTCACTGATGAC TGCCGCTGTGTCATCGTGGGCTCAGCTGCCTACCTCCCAGATGAGCCTCACCCTCCATTTTTTGAGGTA TATCGGAACAGTGAATCAGTGACCCCCAACCCACGGTCCCCTCTAGAAGACTATTCCCTCCATATCATT GACCTTCACACCGGCCGCTTATGTGATACACGCACGTTCAAGTGTGACAAGGTGGTCTTGTCACACAAC CAAGGGCTGTACTTGTACAAAAACATCCTGGCCATCTTGTCTGTGCAACAACAGACCATCCATGTCTTC CAGGTGACTCCTGAAGGCACTTTCATTGATGTGCGGACCATTGGCCGCTTTTGCTATGAGGATGACCTG CTCACTGTGTCAGCTGTTTTCCCTGAGGTACAGCGGGACAGTCAGACAGGCATGGCCAATCCCTTTAGG GATCCTTTCATCAATTCCCTCAAACACCGGTTGCTGGTATATTTGTGGCGCCGGGCAGAACAGGATGGT AGTGCAATGGCCAAGAGGCGCTTCTTCCAGTATTTTGACCAACTGCGGCAGCTGCGAATGTGGAAAATG CAGCTTCTGGATGAAAACCACCTGTTTATCAAGTACACTAGTGAGGATGTAGTAACACTGCGAGTCACA GATCCATCACAGGCATCTTTCTTTGTGGTGTACAATATGGTGACGACAGAGGTGATTGCTGTGTTTGAG AATACATCAGATGAGCTTTTGGAGCTCTTTGAGAACTTCTGTGACCTTTTTCGTAATGCTACCCTGCAC AGTGAAGTTCAGTTTCCCTGCTCAGCTTCTAGCAACAATTTTGCAAGGCAGATCCAGCGCCGGTTCAAA GACACTATTATAAATGCCAAGTATGGAGGGCACACAGAGGCAGTACGCCGGCTGCTGGGTCAGCTCCCC ATCAGTGCTCAGTCTTACAGCGGTAGCCCCTATCTGGATTTGTCTCTCTTCAGTTATGATGACAAGTGG GTATCTGTCATGGAGCGGCCCAAGACTTGTGGAGATCACCCAATCAGGTTCTATGCCCGGGACTCGGGC CTGCTCAAGTTTGAGATCCAGGCGGGGTTATTGGGCCGCCCCATCAACCACACAGTGCGACGCCTTGTT GCCTTCACCTTTCACCCTTTTGAGCCTTTCGCTATTTCTGTGCAGAGGACTAATGCTGAGTATGTTGTC AACTTCCATATGCGACACTGCTGCACGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017996 |
| Insert Size | 1686 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017996.4 |
| RefSeq Size | 2382 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 55070 |
| UniProt ID | Q7L5Y6 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 65.1 kDa |
| Gene Summary | Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216467 | DET1 (Myc-DDK-tagged)-Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1 |
CNY 4,176.00 |
|
| RC216467L1 | Lenti ORF clone of Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1, Myc-DDK-tagged |
CNY 6,576.00 |
|
| RC216467L2 | Lenti ORF clone of Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC216467L3 | Lenti ORF clone of Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC216467L4 | Lenti ORF clone of Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG216467 | DET1 (tGFP-tagged) - Human de-etiolated homolog 1 (Arabidopsis) (DET1), transcript variant 1 |
CNY 5,040.00 |
