Myosin (MYH2) (NM_017534) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IBM3; MYH2A; MYHas8; MyHC-2A; MyHC-IIa; MYHSA2; MYPOP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304476 representing NM_017534. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTTCAGACTCAGAATTGGCTGTTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAG CGCATTGAGGCCCAGAATAGGCCCTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAAGAATCC TTTGTCAAAGGGACCATCCAGAGCAGAGAAGGAGGAAAAGTGACGGTGAAGACTGAGGGAGGAGCGACT CTGACAGTGAAGGATGATCAGGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGATATGGCC ATGATGACTCATCTGCATGAGCCTGCTGTGCTGTACAACCTCAAAGAACGTTATGCAGCCTGGATGATC TACACCTATTCAGGTCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCTGTGTATAAGCCCGAG GTGGTGACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAAC GCCTATCAGTTCATGCTGACTGACCGAGAGAATCAGTCAATCCTGATCACTGGAGAATCTGGTGCAGGG AAGACTGTGAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGTGAGAAGAAGAAG GAAGAAATTACTTCTGGCAAAATACAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTG GAGGCCTTTGGCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGAATC CACTTTGGCACTACTGGAAAACTGGCATCTGCTGATATTGAAACATATCTGCTAGAGAAGTCTAGAGTT GTTTTCCAGCTTAAGGCTGAGAGAAGTTATCATATTTTTTACCAGATTACATCGAATAAGAAACCAGAA CTTATTGAAATGCTTCTGATTACCACGAACCCATATGATTACCCATTTGTCAGTCAAGGGGAGATCAGT GTGGCCAGCATCGATGATCAGGAAGAACTGATGGCCACAGATAGTGCTATTGATATTTTGGGCTTTACT AATGAAGAAAAGGTCTCCATTTACAAGCTCACGGGGGCTGTGATGCATTATGGGAACCTAAAATTTAAG CAAAAGCAGCGTGAGGAGCAAGCAGAGCCAGATGGCACAGAAGTTGCTGACAAGGCGGCCTACCTCCAG AGTCTGAACTCTGCAGATCTGCTCAAAGCTCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTATGTC ACCAAAGGCCAGACTGTAGAACAGGTGTCCAACGCAGTAGGTGCTCTGGCCAAAGCCGTCTACGAGAAG ATGTTCCTGTGGATGGTTGCCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATC GGGGTCTTGGACATTGCTGGTTTTGAGATTTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTC ACCAATGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAG GAAGGCATCGAGTGGACGTTCATCGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAG CCTATGGGCATCTTCTCCATCCTGGAAGAGGAGTGCATGTTCCCTAAGGCAACAGACACCTCCTTCAAG AACAAGCTGTATGACCAGCACCTGGGCAAGTCTGCCAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAAG GCCGAGGCCCACTTCGCTCTGATTCACTATGCTGGTGTTGTGGACTACAACATTACTGGCTGGCTGGAG AAGAACAAGGACCCCCTGAATGAGACCGTGGTTGGACTGTACCAGAAGTCTGCAATGAAAACTCTAGCT CAGCTCTTCTCTGGGGCTCAAACTGCTGAAGGAGAGGGAGCTGGTGGAGGGGCCAAGAAAGGTGGTAAG AAGAAGGGCTCTTCTTTCCAGACAGTGTCTGCCCTTTTCAGAGAGAATTTGAACAAGCTGATGACCAAC CTCAGGAGTACCCATCCTCACTTTGTGAGGTGTATCATCCCCAATGAGACAAAAACTCCTGGTGCCATG GAGCATGAGCTTGTCCTCCACCAGCTGAGGTGTAACGGTGTGCTGGAAGGCATCCGCATCTGTAGGAAA GGATTTCCAAGCAGAATCCTTTATGCAGACTTCAAACAGAGATACAAGGTATTAAATGCAAGTGCAATC CCTGAAGGGCAATTCATTGATAGCAAGAAGGCCTCTGAGAAGCTCCTTGCATCCATCGACATTGACCAC ACCCAGTATAAATTTGGGCACACCAAGGTCTTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATG CGAGATGACAAGCTGGCCCAGCTGATTACCCGAACCCAGGCCAGGTGCAGAGGGTTCTTGGCAAGAGTG GAGTACCAGAGGATGGTGGAGAGAAGGGAGGCCATCTTCTGTATCCAGTACAATATCAGATCCTTCATG AATGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCTCTGTTGAAGAGTGCAGAAACT GAGAAGGAGATGGCCACCATGAAGGAAGAATTTCAGAAAATTAAAGACGAACTTGCCAAGTCAGAGGCA AAAAGGAAGGAACTGGAAGAAAAGATGGTGACGCTGTTGAAAGAAAAAAATGACTTGCAGCTCCAAGTT CAGGCTGAAGCCGAAGGCTTGGCTGATGCAGAGGAAAGGTGTGACCAGCTAATCAAAACCAAAATCCAG CTAGAAGCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGGATGAGGAAGAGATCAATGCTGAGCTGACA GCCAAGAAGAGGAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACATTGATGACCTTGAGCTGACA CTGGCCAAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAGGTGAAAAACCTCACAGAAGAGATGGCA GGTCTGGATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCCAGGAGGCCCACCAGCAGACC CTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCTAAAATCAAACTTGAACAA CAAGTGGATGATCTTGAAGGGTCCTTGGAGCAAGAAAAGAAACTTCGCATGGACCTAGAAAGGGCTAAG AGGAAACTTGAGGGTGACTTGAAGTTGGCCCAAGAATCCATAATGGACATTGAAAATGAGAAACAGCAA CTTGATGAAAAGCTCAAAAAGAAAGAGTTTGAAATCAGCAATCTGCAAAGCAAGATTGAAGATGAACAG GCACTTGGCATTCAATTGCAGAAGAAAATTAAAGAATTGCAAGCCCGCATTGAGGAGCTGGAGGAGGAA ATCGAGGCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCTCCCGGGAGCTGGAG GAGATCAGCGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGATTGAGATGAACAAGAAGCGG GAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAGCATGAAGCCACAGCGGCC ACCCTGAGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGAGTG AAGCAGAAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATTGATGACCTTGCTAGTAATGTAGAA ACGGTCTCCAAAGCCAAGGGAAACCTAGAGAAAATGTGCCGGACTCTAGAGGACCAACTGAGTGAACTG AAATCAAAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTGACTGCGCAGAGGGGGCGCCTGCAGACT GAATCTGGTGAGTTTTCACGCCAGCTTGATGAAAAGGAAGCTCTGGTGTCTCAGTTATCAAGAGGCAAA CAAGCCTTTACTCAACAGATTGAAGAATTAAAGAGGCAACTTGAAGAGGAGATAAAAGCCAAGAACGCC CTGGCGCATGCCCTGCAGTCTTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAG GAATCCAAGGCCGAGCTGCAGAGAGCACTGTCCAAGGCCAACACCGAGGTTGCCCAATGGAGGACCAAA TACGAGACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCCCAGCGGCTG CAGGCAGCTGAGGAACATGTAGAAGCTGTGAACGCCAAATGTGCTTCCCTCGAAAAGACGAAGCAGCGG CTGCAGAATGAGGTCGAGGACCTCATGCTTGATGTGGAGAGGACAAATGCCGCCTGTGCCGCCCTTGAC AAAAAGCAAAGGAACTTCGATAAGATCCTGGCAGAATGGAAACAGAAATGTGAGGAAACGCATGCTGAG CTTGAGGCCTCCCAGAAGGAGGCCCGTTCCCTTGGCACTGAGCTGTTCAAGATAAAGAATGCCTATGAG GAATCTTTGGATCAGCTAGAAACCCTGAAGCGAGAGAACAAAAACTTACAGCAGGAGATTTCTGACCTC ACGGAACAGATTGCAGAAGGAGGGAAACGTATCCATGAACTGGAGAAAATAAAGAAACAAGTGGAACAA GAAAAGTGTGAACTTCAGGCTGCTTTAGAAGAAGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGATC CTGCGCATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGAG GAAATTGACCAGCTGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACGCTGGATGCTGAG ATCAGGAGTAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATC CAGCTGAACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTACAGGAACACCCAAGGCATCCTC AAGGATACCCAGATCCACCTGGATGATGCTCTCCGGAGCCAGGAGGACCTGAAGGAACAGCTGGCCATG GTGGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAACAGACAGAG AGGAGCAGAAAAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTTCAGCTACTGCACACCCAG AACACCAGCCTGATCAACACCAAGAAGAAGCTGGAGACAGATATTTCCCAAATGCAAGGAGAGATGGAG GACATTCTCCAGGAAGCCCGCAATGCAGAAGAAAAGGCCAAGAAGGCCATCACTGATGCCGCCATGATG GCTGAGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAG ACCGTGAAGGATCTGCAGCTCCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAG ATCCAGAAACTGGAGGCCAGGGTACGGGAGCTGGAAGGAGAGGTTGAGAGTGAGCAAAAGCGTAATGCT GAGGCTGTCAAAGGTCTGCGCAAACATGAGAGGCGAGTGAAGGAACTCACTTACCAGACGGAAGAAGAT AGAAAGAATATTCTCAGGCTTCAAGATTTGGTAGATAAACTTCAGGCAAAAGTGAAATCTTATAAGAGA CAAGCTGAGGAGGCTGAGGAACAATCCAACACCAATCTAGCTAAATTCCGCAAGCTCCAGCATGAGCTG GAGGAGGCCGAGGAACGGGCTGACATTGCTGAGTCCCAGGTGAACAAACTGCGGGTGAAGAGCCGGGAG GTTCACACAAAAGTCATAAGTGAAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_017534
Insert Size	5826 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017534.5
RefSeq Size	6163 bp
RefSeq ORF	5826 bp
Locus ID	4620
UniProt ID	Q9UKX2
Protein Pathways	Tight junction, Viral myocarditis
MW	223 kDa
Gene Summary	Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants encode the same protein.

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