ARID1B (NM_017519) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	6A3-5; BAF250B; BRIGHT; CSS1; DAN15; ELD/OSA1; MRD12; OSA2; P250R
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304471 representing NM_017519. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCATAACGCGGGCGCCGCGGCCGCCGCCGGCACCCACAGCGCCAAGAGCGGCGGCTCCGAGGCG GCTCTCAAGGAGGGTGGAAGCGCCGCCGCGCTGTCCTCCTCCTCCTCCTCCTCCGCGGCGGCAGCGGCG GCATCCTCTTCCTCCTCGTCGGGCCCGGGCTCGGCCATGGAGACGGGGCTGCTCCCCAACCACAAACTG AAAACCGTTGGCGAAGCCCCCGCCGCGCCGCCCCACCAGCAGCACCACCACCACCACCATGCCCACCAC CACCACCACCATGCCCACCACCTCCACCACCACCACGCACTACAGCAGCAGCTAAACCAGTTCCAGCAG CAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAACATCCCATTTCCAACAACAAC AGCTTGGGCGGCGCGGGCGGCGGCGCGCCTCAGCCCGGCCCCGACATGGAGCAGCCGCAACATGGAGGC GCCAAGGACAGTGCTGCGGGCGGCCAGGCCGACCCCCCGGGCCCGCCGCTGCTGAGCAAGCCGGGCGAC GAGGACGACGCGCCGCCCAAGATGGGGGAGCCGGCGGGCGGCCGCTACGAGCACCCGGGCTTGGGCGCC CTGGGCACGCAGCAGCCGCCGGTCGCCGTGCCCGGGGGCGGCGGCGGCCCGGCGGCCGTCCCGGAGTTT AATAATTACTATGGCAGCGCTGCCCCTGCGAGCGGCGGCCCCGGCGGCCGCGCTGGGCCTTGCTTTGAT CAACATGGCGGACAACAAAGCCCCGGGATGGGGATGATGCACTCCGCCTCCGCCGCCGCCGCCGGGGCC CCCGGCAGCATGGACCCCCTGCAGAACTCCCACGAAGGGTACCCCAACAGCCAGTGCAACCATTATCCG GGCTACAGCCGGCCCGGCGCGGGCGGCGGCGGCGGCGGCGGCGGCGGAGGAGGAGGAGGCAGCGGAGGA GGAGGAGGAGGAGGAGGAGCAGGAGCAGGAGGAGCAGGAGCGGGAGCTGTGGCGGCGGCGGCCGCGGCG GCGGCGGCAGCAGCAGGAGGCGGCGGCGGCGGCGGCTATGGGGGCTCGTCCGCGGGGTACGGGGTGCTG AGCTCCCCCCGGCAGCAGGGCGGCGGCATGATGATGGGCCCCGGGGGCGGCGGGGCCGCGAGCCTCAGC AAGGCGGCCGCCGGCTCGGCGGCGGGGGGCTTCCAGCGCTTCGCCGGCCAGAACCAGCACCCGTCGGGG GCCACCCCGACCCTCAATCAGCTGCTCACCTCGCCCAGCCCCATGATGCGGAGCTACGGCGGCAGCTAC CCCGAGTACAGCAGCCCCAGCGCGCCGCCGCCGCCGCCGTCGCAGCCCCAGTCCCAGGCGGCGGCGGCG GGGGCGGCGGCGGGCGGCCAGCAGGCGGCCGCGGGCATGGGCTTGGGCAAGGACATGGGCGCCCAGTAC GCCGCTGCCAGCCCGGCCTGGGCGGCCGCGCAACAAAGGAGTCACCCGGCGATGAGCCCCGGCACCCCC GGACCGACCATGGGCAGATCCCAGGGCAGCCCAATGGATCCAATGGTGATGAAGAGACCTCAGTTGTAT GGCATGGGCAGTAACCCTCATTCTCAGCCTCAGCAGAGCAGTCCGTACCCAGGAGGTTCCTATGGCCCT CCAGGCCCACAGCGGTATCCAATTGGCATCCAGGGTCGGACTCCCGGGGCCATGGCCGGAATGCAGTAC CCTCAGCAGCAGATGCCACCTCAGTATGGACAGCAAGGTGTGAGTGGTTACTGCCAGCAGGGCCAACAG CCATATTACAGCCAGCAGCCGCAGCCCCCGCACCTCCCACCCCAGGCGCAGTATCTGCCGTCCCAGTCC CAGCAGAGGTACCAGCCGCAGCAGGACATGTCTCAGGAAGGCTATGGAACTAGATCTCAACCTCCTCTG GCCCCCGGAAAACCTAACCATGAAGACTTGAACTTAATACAGCAAGAAAGACCATCAAGTTTACCAGAT CTGTCTGGCTCCATTGATGACCTCCCCACGGGAACGGAAGCAACTTTGAGCTCAGCAGTCAGTGCATCC GGGTCCACGAGCAGCCAAGGGGATCAGAGCAACCCGGCGCAGTCGCCTTTCTCCCCACATGCGTCCCCT CATCTCTCCAGCATCCCGGGGGGCCCATCTCCCTCTCCTGTTGGCTCTCCTGTAGGAAGCAACCAGTCT CGATCTGGCCCAATCTCTCCTGCAAGTATCCCAGGTAGTCAGATGCCTCCGCAGCCACCCGGGAGCCAG TCAGAATCCAGTTCCCATCCCGCCTTGAGCCAGTCACCAATGCCACAGGAAAGAGGTTTTATGGCAGGC ACACAAAGAAACCCTCAGATGGCTCAGTATGGACCTCAACAGACAGGACCATCCATGTCGCCTCATCCT TCTCCTGGGGGCCAGATGCATGCTGGAATCAGTAGCTTTCAGCAGAGTAACTCAAGTGGGACTTACGGT CCACAGATGAGCCAGTATGGACCACAAGGTAACTACTCCAGACCCCCAGCGTATAGTGGGGTGCCCAGT GCAAGCTACAGCGGCCCAGGGCCCGGTATGGGTATCAGTGCCAACAACCAGATGCATGGACAAGGGCCA AGCCAGCCATGTGGTGCTGTGCCCCTGGGACGAATGCCATCAGCTGGGATGCAGAACAGACCATTTCCT GGAAATATGAGCAGCATGACCCCCAGTTCTCCTGGCATGTCTCAGCAGGGAGGGCCAGGAATGGGGCCG CCAATGCCAACTGTGAACCGTAAGGCACAGGAGGCAGCCGCAGCAGTGATGCAGGCTGCTGCGAACTCA GCACAAAGCAGGCAAGGCAGTTTCCCCGGCATGAACCAGAGTGGACTTATGGCTTCCAGCTCTCCCTAC AGCCAGCCCATGAACAACAGCTCTAGCCTGATGAACACGCAGGCGCCGCCCTACAGCATGGCGCCCGCC ATGGTGAACAGCTCGGCAGCATCTGTGGGTCTTGCAGATATGATGTCTCCTGGTGAATCCAAACTGCCC CTGCCTCTCAAAGCAGACGGCAAAGAAGAAGGCACTCCACAGCCCGAGAGCAAGTCAAAGAAGTCCAGC TCCTCCACCACTACTGGGGAGAAGATCACGAAGGTGTACGAGCTGGGGAATGAGCCAGAGAGAAAGCTC TGGGTCGACCGATACCTCACCTTCATGGAAGAGAGAGGCTCTCCTGTCTCAAGTCTGCCTGCCGTGGGC AAGAAGCCCCTGGACCTGTTCCGACTCTACGTCTGCGTCAAAGAGATCGGGGGTTTGGCCCAGGTTAAT AAAAACAAGAAGTGGCGTGAGCTGGCAACCAACCTAAACGTTGGCACCTCAAGCAGTGCAGCGAGCTCC CTGAAAAAGCAGTATATTCAGTACCTGTTTGCCTTTGAGTGCAAGATCGAACGTGGGGAGGAGCCCCCG CCGGAAGTCTTCAGCACCGGGGACACCAAAAAGCAGCCCAAGCTCCAGCCGCCATCTCCTGCTAACTCG GGATCCTTGCAAGGCCCACAGACCCCCCAGTCAACTGGCAGCAATTCCATGGCAGAGGTTCCAGGTGAC CTGAAGCCACCTACCCCAGCCTCCACCCCTCACGGCCAGATGACTCCAATGCAAGGTGGAAGAAGCAGT ACAATCAGTGTGCACGACCCATTCTCAGATGTGAGTGATTCATCCTTCCCGAAACGGAACTCCATGACT CCAAACGCCCCCTACCAGCAGGGCATGAGCATGCCCGATGTGATGGGCAGGATGCCCTATGAGCCCAAC AAGGACCCCTTTGGGGGAATGAGAAAAGTGCCTGGAAGCAGCGAGCCCTTTATGACGCAAGGACAGATG CCCAACAGCAGCATGCAGGACATGTACAACCAAAGTCCCTCCGGAGCAATGTCTAACCTGGGCATGGGG CAGCGCCAGCAGTTTCCCTATGGAGCCAGTTACGACCGAAGGCATGAACCTTATGGGCAGCAGTATCCA GGCCAAGGCCCTCCCTCGGGACAGCCGCCGTATGGAGGGCACCAGCCCGGCCTGTACCCACAGCAGCCG AATTACAAACGCCATATGGACGGCATGTACGGGCCCCCAGCCAAGCGCCACGAGGGCGACATGTACAAC ATGCAGTACAGCAGCCAGCAGCAGGAGATGTACAACCAGTATGGAGGCTCCTACTCGGGCCCGGACCGC AGGCCCATCCAGGGCCAGTACCCGTATCCCTACAGCAGGGAGAGGATGCAGGGCCCGGGGCAGATCCAG ACACACGGAATCCCGCCTCAGATGATGGGCGGCCCGCTGCAGTCGTCCTCCAGTGAGGGGCCTCAGCAG AATATGTGGGCAGCACGCAATGATATGCCTTATCCCTACCAGAACAGGCAGGGCCCTGGCGGCCCTACA CAGGCGCCCCCTTACCCAGGCATGAACCGCACAGACGATATGATGGTACCCGATCAGAGGATAAATCAT GAGAGCCAGTGGCCTTCTCACGTCAGCCAGCGTCAGCCTTATATGTCGTCCTCAGCCTCCATGCAGCCC ATCACACGCCCACCACAGCCGTCCTACCAGACGCCACCGTCACTGCCAAATCACATCTCCAGGGCGCCC AGCCCAGCGTCCTTCCAGCGCTCCCTGGAGAACCGCATGTCTCCAAGCAAGTCTCCTTTTCTGCCGTCT ATGAAGATGCAGAAGGTCATGCCCACGGTCCCCACATCCCAGGTCACCGGGCCACCACCCCAACCACCC CCAATCAGAAGGGAGATCACCTTTCCTCCTGGCTCAGTAGAAGCATCACAACCAGTCTTGAAACAAAGG CGAAAGATTACCTCCAAAGATATCGTTACTCCTGAGGCGTGGCGTGTGATGATGTCCCTTAAATCAGGT CTTTTGGCTGAGAGTACGTGGGCTTTGGACACTATTAATATTCTTCTGTATGATGACAGCACTGTTGCT ACTTTCAATCTCTCCCAGTTGTCTGGATTTCTCGAACTTTTAGTCGAGTACTTTAGAAAATGCCTGATT GACATTTTTGGAATTCTTATGGAATATGAAGTGGGAGACCCCAGCCAAAAAGCACTTGATCACAACGCA GCAAGGAAGGATGACAGCCAGTCCTTGGCAGACGATTCTGGGAAAGAGGAGGAAGATGCTGAATGTATT GATGACGACGAGGAAGACGAGGAGGATGAGGAGGAAGACAGCGAGAAGACAGAAAGCGATGAAAAGAGC AGCATCGCTCTGACTGCCCCGGACGCCGCTGCAGACCCAAAGGAGAAGCCCAAGCAAGCCAGTAAGTTC GACAAGCTGCCAATAAAGATAGTCAAAAAGAACAACCTGTTTGTTGTTGACCGATCTGACAAGTTGGGG CGTGTGCAGGAGTTCAATAGTGGCCTTCTGCACTGGCAGCTCGGCGGGGGTGACACCACCGAGCACATT CAGACTCACTTTGAGAGCAAGATGGAAATTCCTCCTCGCAGGCGCCCACCTCCCCCCTTAAGCTCCGCA GGTAGAAAGAAAGAGCAAGAAGGCAAAGGCGACTCTGAAGAGCAGCAAGAGAAAAGCATCATAGCAACC ATCGATGACGTCCTCTCTGCTCGGCCAGGGGCATTGCCTGAAGACGCAAACCCTGGGCCCCAGACCGAA AGCAGTAAGTTTCCCTTTGGTATCCAGCAAGCCAAAAGTCACCGGAACATCAAGCTGCTGGAGGACGAG CCCAGGAGCCGAGACGAGACTCCTCTGTGTACCATCGCGCACTGGCAGGACTCGCTGGCTAAGCGATGC ATCTGTGTGTCCAATATTGTCCGTAGCTTGTCATTCGTGCCTGGCAATGATGCCGAAATGTCCAAACAT CCAGGCCTGGTGCTGATCCTGGGGAAGCTGATTCTTCTTCACCACGAGCATCCAGAGAGAAAGCGAGCA CCGCAGACCTATGAGAAAGAGGAGGATGAGGACAAGGGGGTGGCCTGCAGCAAAGATGAGTGGTGGTGG GACTGCCTCGAGGTCTTGAGGGATAACACGTTGGTCACGTTGGCCAACATTTCCGGGCAGCTAGACTTG TCTGCTTACACGGAAAGCATCTGCTTGCCAATTTTGGATGGCTTGCTGCACTGGATGGTGTGCCCGTCT GCAGAGGCACAAGATCCCTTTCCAACTGTGGGACCCAACTCGGTCCTGTCGCCTCAGAGACTTGTGCTG GAGACCCTCTGTAAACTCAGTATCCAGGACAATAATGTGGACCTGATCTTGGCCACTCCTCCATTTAGT CGTCAGGAGAAATTCTATGCTACATTAGTTAGGTACGTTGGGGATCGCAAAAACCCAGTCTGTCGAGAA ATGTCCATGGCGCTTTTATCGAACCTTGCCCAAGGGGACGCACTAGCAGCAAGGGCCATAGCTGTGCAG AAAGGAAGCATTGGAAACTTGATAAGCTTCCTAGAGGATGGGGTCACGATGGCCCAGTACCAGCAGAGC CAGCACAACCTCATGCACATGCAGCCCCCGCCCCTGGAACCACCTAGCGTAGACATGATGTGCAGGGCG GCCAAGGCTTTGCTAGCCATGGCCAGAGTGGACGAAAACCGCTCGGAATTCCTTTTGCACGAGGGCCGG TTGCTGGATATCTCGATATCAGCTGTCCTGAACTCTCTGGTTGCATCTGTCATCTGTGATGTACTGTTT CAGATTGGGCAGTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_017519
Insert Size	6711 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017519.2
RefSeq Size	9609 bp
RefSeq ORF	6711 bp
Locus ID	57492
UniProt ID	Q8NFD5
MW	236.1 kDa
Gene Summary	This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 3. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.