DFNB31 (WHRN) (NM_015404) Human Untagged Clone

CAT#: SC304310

DFNB31 (untagged)-Human deafness, autosomal recessive 31 (DFNB31), transcript variant 1



  "NM_015404" in other vectors (4)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
WHRN Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CIP98; DFNB31; PDZD7B; USH2D; WI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC304310 representing NM_015404.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACGCGCCGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCCGGG
GCGGGCGGCGGCGGGGGCGCGGGGCTGCGGTTACTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCTG
ACCGCGCTGCTGAGCGAGGCGGAGCGGGAGCAGTTCACCCACTGCCTGAACGCTTACCACGCGCGCCGC
AACGTCTTCGACCTGGTGCGCACCCTGCGCGTGCTGCTGGACAGTCCGGTCAAGCGGCGCCTGCTGCCC
ATGCTTCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAATACACGGCCGAGGGCCTCTAC
CTGCCCGCCACCACCCCCTACAGGCAGCCCGCCTGGGGCGGCCCCGACAGCGCGGGGCCAGGGGAGGTG
CGCCTGGTGAGTTTGCGGCGTGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGTGGGGGCTCGGAG
CACGGCGTGGGCATCTACGTGTCTCTGGTGGAACCAGGCTCTCTAGCTGAGAAGGAAGGACTGCGGGTC
GGGGACCAGATTCTGCGCGTCAACGACAAATCCCTGGCCCGGGTGACCCACGCGGAGGCCGTCAAGGCT
CTGAAGGGCTCCAAGAAGCTGGTGCTGTCTGTGTACTCAGCAGGGCGCATCCCTGGGGGCTACGTCACC
AACCACATCTACACCTGGGTGGACCCGCAGGGCCGCAGCATCTCCCCACCCTCGGGCCTGCCCCAGCCC
CACGGTGGTGCCCTGAGGCAGCAGGAGGGTGACCGGAGGAGCACCCTGCACCTCCTGCAAGGAGGGGAT
GAGAAAAAGGTGAACCTGGTGCTGGGGGACGGCCGGTCCCTGGGCCTCACGATCCGTGGGGGAGCTGAG
TACGGCCTTGGCATTTACATCACTGGCGTGGACCCAGGCTCTGAAGCAGAAGGCAGCGGGCTCAAGGTT
GGGGACCAGATTCTAGAAGTGAATGGGCGGAGCTTTCTCAACATCCTACACGACGAGGCTGTCAGGCTG
CTTAAGTCATCTCGGCACCTCATCCTGACAGTGAAGGACGTCGGGAGGCTGCCCCATGCCCGCACCACT
GTGGACGAGACCAAGTGGATCGCCAGTTCCCGGATCAGGGAGACCATGGCGAACTCGGCAGGGTTTCTT
GGCGATCTCACAACAGAAGGAATAAACAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACC
CTGAGCAGCCTGGGGAACCAGACACGAGTGCTGCTGGAGGAGCAGGCTCGGCACCTGCTGAACGAGCAG
GAACACGCCACCATGGCCTACTACCTGGATGAGTACCGTGGTGGCAGCGTCTCTGTGGAGGCCCTCGTC
ATGGCCCTGTTCAAGCTGCTCAACACCCACGCCAAGTTCTCACTCCTCTCTGAGGTGAGAGGCACCATT
TCCCCGCAAGACCTAGAACGCTTCGACCACCTGGTGCTGAGGCGTGAGATTGAGTCCATGAAGGCGCGG
CAGCCCCCAGGCCCCGGGGCTGGGGACACCTACTCCATGGTCTCCTACAGTGACACGGGTTCATCCACA
GGCAGCCACGGCACCTCCACCACCGTCAGCTCGGCCAGGAACACTCTGGACCTGGAGGAAACTGGCGAG
GCTGTCCAGGGCAATATCAACGCCCTCCCAGATGTGTCCGTGGATGATGTCAGATCCACCTCCCAGGGG
CTGTCAAGCTTCAAGCCACTGCCTCGCCCACCACCTCTGGCCCAAGGCAACGACCTCCCACTAGGCCAG
CCAAGGAAGCTGGGGAGAGAGGACCTCCAGCCACCTTCCTCCATGCCTTCCTGCTCGGGCACTGTCTTC
TCGGCTCCACAGAACCGCAGCCCGCCAGCGGGCACCGCACCCACCCCAGGGACCTCCTCTGCACAGGAC
TTGCCCTCTTCCCCCATCTATGCCTCCGTCTCCCCTGCCAACCCCAGCTCCAAGAGGCCGCTGGACGCC
CATCTGGCCCTGGTCAACCAACACCCCATCGGCCCCTTCCCACGGGTCCAGTCACCCCCGCACCTGAAA
AGCCCCTCTGCAGAGGCCACAGTGGCTGGGGGCTGCCTTCTGCCCCCATCACCCTCTGGCCACCCAGAC
CAGACAGGCACAAACCAGCACTTTGTCATGGTGGAGGTCCACCGCCCCGACAGCGAGCCAGACGTCAAT
GAAGTGAGGGCGCTGCCCCAGACGCGCACAGCCTCTACGCTCTCCCAGCTCTCGGACAGCGGGCAGACT
CTAAGCGAGGACAGTGGTGTGGATGCTGGCGAGGCAGAGGCCAGCGCCCCAGGCCGAGGAAGGCAGTCG
GTGTCCACCAAGAGCAGGAGTAGCAAGGAGCTGCCTCGGAACGAGAGGCCCACAGATGGGGCCAACAAA
CCGCCTGGACTTCTGGAGCCCACGTCCACTCTGGTCCGTGTGAAGAAAAGTGCGGCCACCCTGGGCATC
GCCATCGAGGGTGGCGCCAACACCCGCCAGCCCCTGCCTAGGATTGTCACTATTCAGAGAGGCGGCTCA
GCTCACAACTGTGGGCAGCTCAAGGTGGGCCACGTGATTCTGGAAGTGAATGGGCTGACGCTTCGGGGC
AAGGAGCACCGGGAGGCCGCCCGCATTATCGCCGAGGCCTTCAAGACTAAGGACCGTGACTACATTGAC
TTTCTGGTCACTGAGTTCAATGTGATGCTCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_015404
Insert Size 2724 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015404.3
RefSeq Size 4079 bp
RefSeq ORF 2724 bp
Locus ID 25861
UniProt ID Q9P202
MW 96.6 kDa
Gene Summary This gene is thought to function in the organization and stabilization of sterocilia elongation and actin cystoskeletal assembly, based on studies of the related mouse gene. Mutations in this gene have been associated with autosomal recessive non-syndromic deafness and Usher Syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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