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CRYGA (NM_014617) Human Untagged Clone
CAT＃: SC304141
CRYGA (untagged)-Human crystallin, gamma A (CRYGA)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRY-g-A; CRYG1; CRYG5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304141 representing NM_014617.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAAGATCACCTTCTACGAGGACCGAGACTTTCAGGGTCGCTGCTACAATTGCATCAGTGACTGC CCCAACCTGCGGGTCTACTTCAGCCGCTGCAACTCCATCCGAGTAGACAGCGGCTGCTGGATGCTCTAT GAGCGTCCCAATTACCAGGGCCACCAGTACTTCCTGCGCCGAGGCAAGTACCCCGACTATCAGCACTGG ATGGGCCTCAGCGACTCGGTCCAATCCTGCCGTATAATTCCTCATACCAGCTCGCACAAGTTAAGGCTG TACGAGAGAGATGACTACCGAGGCCTTATGTCTGAGCTCACTGATGACTGCGCCTGTGTTCCAGAACTG TTCCGTCTCCCTGAGATCTATTCCCTCCACGTACTGGAGGGCTGCTGGGTCCTCTATGAAATGCCCAAC TACCGGGGGCGGCAGTATCTGCTGAGGCCTGGGGACTACAGAAGGTACCACGACTGGGGGGGTGCAGAT GCCAAAGTCGGCTCTTTGAGACGGGTCACCGATTTGTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014617 |
| Insert Size | 525 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014617.3 |
| RefSeq Size | 608 bp |
| RefSeq ORF | 525 bp |
| Locus ID | 1418 |
| UniProt ID | P11844 |
| Protein Families | Druggable Genome |
| MW | 20.9 kDa |
| Gene Summary | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224787 | CRYGA (Myc-DDK-tagged)-Human crystallin, gamma A (CRYGA) |
CNY 2,400.00 |
|
| RC224787L1 | Lenti ORF clone of Human crystallin, gamma A (CRYGA), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC224787L2 | Lenti ORF clone of Human crystallin, gamma A (CRYGA), mGFP tagged |
CNY 5,890.00 |
|
| RC224787L3 | Lenti ORF clone of Human crystallin, gamma A (CRYGA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224787L4 | Lenti ORF clone of Human crystallin, gamma A (CRYGA), mGFP tagged |
CNY 5,890.00 |
|
| RG224787 | CRYGA (tGFP-tagged) - Human crystallin, gamma A (CRYGA) |
CNY 4,370.00 |
