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PFAS (NM_012393) Human Untagged Clone
CAT＃: SC303975
PFAS (untagged)-Human phosphoribosylformylglycinamidine synthase (PFAS)
CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FGAMS; FGAR-AT; FGARAT; GATD8; PURL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303975 representing NM_012393.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCCCAGTCCTTCACTTCTATGTTCGTCCCTCTGGCCATGAGGGGGCAGCCCCTGGACACACTCGG AGGAAACTGCAAGGGAAACTGCCAGAGCTGCAGGGCGTCGAGACTGAACTGTGCTACAACGTGAACTGG ACAGCTGAGGCCCTCCCCAGTGCTGAGGAGACAAAGAAGCTGATGTGGCTGTTTGGTTGCCCCTTACTG CTGGATGATGTTGCTCGGGAGTCCTGGCTCCTTCCTGGCTCCAATGACCTGCTGCTGGAGGTCGGGCCC AGGCTGAACTTCTCCACCCCAACATCCACCAACATCGTGTCAGTGTGCCGCGCCACTGGGCTGGGGCCT GTGGATCGTGTGGAGACCACCCGGCGCTACCGGCTCTCGTTTGCCCACCCCCCGTCAGCTGAGGTGGAA GCCATTGCTCTGGCTACCCTGCACGACCGGATGACAGAGCAGCACTTCCCCCATCCCATCCAGAGTTTC TCCCCTGAGAGCATGCCGGAACCCCTCAATGGCCCTATCAATATACTGGGTGAGGGCCGGCTTGCGCTG GAGAAGGCCAACCAGGAGCTTGGTCTGGCTTTAGACTCTTGGGACCTAGACTTCTACACCAAGCGCTTC CAGGAGCTACAGCGGAACCCGAGCACTGTGGAGGCCTTTGACTTGGCGCAGTCCAATAGCGAGCACAGC CGACACTGGTTCTTCAAGGGCCAGCTCCACGTGGATGGGCAGAAGCTGGTGCACTCACTGTTTGAGTCC ATCATGAGCACCCAGGAATCCTCGAACCCCAACAACGTCCTCAAATTCTGTGATAACAGCAGTGCAATC CAGGGAAAGGAAGTCCGATTCCTACGGCCTGAGGACCCCACACGGCCAAGCCGCTTCCAGCAACAGCAA GGGCTGAGACATGTTGTCTTCACAGCAGAGACTCACAACTTTCCCACAGGAGTATGCCCCTTTAGTGGT GCAACCACTGGCACAGGGGGCCGGATTCGAGATGTCCAGTGCACAGGCCGCGGGGCCCACGTGGTGGCT GGCACTGCCGGCTATTGCTTTGGAAATCTGCATATTCCAGGTTACAATCTGCCCTGGGAGGATCCAAGC TTCCAGTATCCTGGGAATTTTGCCCGGCCCCTGGAGGTTGCCATTGAAGCCAGTAATGGAGCTTCTGAC TATGGCAACAAGTTTGGGGAACCAGTGCTGGCTGGCTTCGCCCGCTCCTTGGGCCTCCAGCTCCCAGAC GGCCAGCGGCGTGAGTGGATCAAGCCCATCATGTTTAGTGGGGGCATTGGGTCCATGGAAGCTGACCAC ATAAGCAAGGAGGCCCCAGAGCCAGGCATGGAAGTTGTAAAGGTTGGAGGTCCCGTCTACAGGATTGGA GTTGGAGGTGGAGCTGCTTCATCTGTGCAGGTGCAGGGAGATAACACCAGTGACCTGGACTTTGGGGCT GTGCAGCGAGGAGACCCGGAGATGGAACAGAAGATGAACCGTGTGATCAGGGCTTGTGTGGAGGCCCCC AAGGGAAACCCCATCTGCAGCCTTCATGATCAGGGCGCTGGTGGCAATGGCAATGTCCTAAAAGAGCTG AGTGACCCAGCTGGAGCCATCATTTACACCAGCCGCTTCCAGCTTGGGGACCCAACCCTGAATGCCCTG GAAATCTGGGGGGCTGAGTACCAGGAATCAAATGCTCTTCTGCTGAGGTCCCCCAACCGGGACTTCCTG ACTCATGTCAGTGCCCGTGAACGTTGCCCGGCTTGCTTCGTGGGCACCATCACTGGAGACCGGAGAATA GTGCTGGTGGACGATCGGGAGTGTCCTGTCAGAAGAAATGGCCAGGGGGATGCCCCCCCGACACCCCTG CCAACCCCTGTGGACCTGGAGCTCGAATGGGTGCTGGGCAAGATGCCTCGGAAGGAGTTCTTCCTGCAG AGGAAGCCCCCCATGCTGCAGCCTCTGGCCTTGCCCCCAGGGCTGAGCGTGCACCAGGCTCTGGAGAGG GTTCTGAGGCTGCCCGCCGTGGCCAGCAAGCGCTACCTCACCAATAAGGTGGACCGCTCTGTGGGAGGC CTGGTGGCCCAGCAGCAGTGCGTGGGGCCCCTGCAAACTCCTCTGGCAGATGTAGCGGTTGTGGCACTG AGCCATGAGGAGCTCATAGGGGCTGCCACAGCCTTGGGAGAACAGCCAGTCAAGAGCCTGCTGGACCCA AAAGTCGCCGCCCGGCTGGCCGTGGCCGAAGCCCTCACCAACCTGGTGTTTGCTCTGGTCACTGACCTC CGGGATGTGAAGTGTAGCGGGAACTGGATGTGGGCAGCCAAGCTCCCAGGGGAGGGCGCAGCTTTGGCG GATGCCTGTGAGGCTATGGTGGCAGTGATGGCAGCCCTGGGTGTGGCAGTGGATGGTGGCAAGGACTCC CTCAGCATGGCTGCTCGGGTTGGCACTGAGACCGTGCGGGCTCCTGGGTCACTGGTCATCTCAGCCTAT GCCGTCTGCCCAGACATCACAGCCACTGTGACCCCAGACCTCAAGCATCCTGAAGGGAGAGGCCATCTG CTCTATGTGGCTCTGAGCCCTGGGCAGCACCGGCTCGGGGGCACAGCTCTGGCCCAGTGCTTCTCCCAG CTTGGGGAACACCCTCCAGACCTGGACCTTCCTGAGAACTTGGTGCGGGCCTTCAGCATCACTCAGGGG CTGCTGAAAGACCGCCTCCTCTGCTCAGGCCACGATGTCAGTGACGGAGGCCTCGTCACATGCCTGCTG GAGATGGCCTTTGCTGGAAATTGCGGGCTACAGGTGGATGTGCCTGTCCCCAGGGTTGATGTCCTGTCT GTGCTGTTCGCTGAGGAGCCAGGCCTCGTGCTGGAGGTGCAGGAGCCAGACCTGGCCCAGGTGCTGAAG CGTTACCGGGATGCTGGCCTCCATTGCCTGGAGCTGGGCCACACAGGCGAGGCCGGGCCCCACGCCATG GTCCGGGTGTCAGTGAACGGGGCTGTGGTTCTGGAGGAGCCTGTTGGGGAGCTGCGAGCCCTCTGGGAG GAGACGAGTTTCCAGCTGGACCGGCTACAGGCAGAGCCTCGCTGTGTGGCAGAGGAGGAACGGGGCCTG AGGGAGCGGATGGGGCCCAGCTATTGCCTGCCCCCCACCTTTCCCAAAGCCTCCGTGCCCCGTGAGCCT GGTGGTCCCAGCCCCCGAGTCGCCATCTTGCGAGAGGAGGGCAGTAATGGAGACCGGGAGATGGCCGAT GCCTTCCACTTAGCTGGGTTTGAGGTATGGGACGTGACCATGCAGGACCTCTGCTCTGGGGCAATTGGG CTGGACACTTTCCGTGGCGTGGCCTTCGTGGGCGGCTTCAGCTATGCAGATGTCCTGGGCTCTGCCAAA GGGTGGGCAGCTGCTGTGACCTTTCATCCCAGGGCTGGGGCTGAGCTGAGGCGCTTCCGGAAGCGGCCA GACACCTTCAGCCTGGGCGTGTGTAATGGCTGTCAACTGCTGGCTCTGCTCGGCTGGGTGGGAGGCGAC CCCAATGAGGATGCTGCAGAGATGGGCCCTGACTCCCAGCCAGCCCGGCCAGGCCTTCTGCTACGCCAC AACCTGTCTGGGCGCTACGAGTCTCGCTGGGCCAGCGTGCGTGTGGGGCCTGGGCCAGCCCTGATGCTG CGAGGGATGGAGGGCGCCGTGCTGCCCGTGTGGAGTGCGCACGGGGAAGGTTACGTAGCATTTTCTTCT CCGGAACTCCAAGCTCAGATTGAGGCCAGGGGCTTGGCTCCACTGCACTGGGCTGATGATGACGGGAAC CCCACAGAGCAGTACCCTCTGAATCCCAATGGGTCCCCAGGGGGCGTGGCTGGCATCTGCTCCTGTGAT GGCCGCCACCTGGCTGTCATGCCTCACCCTGAGCGGGCCGTTAGGCCTTGGCAGTGGGCATGGCGACCC CCTCCATTTGATACTCTGACCACCTCCCCCTGGCTCCAGCTCTTTATCAATGCCCGAAACTGGACCCTG GAAGGGAGCTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_012393 |
| Insert Size | 4017 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012393.2 |
| RefSeq Size | 5400 bp |
| RefSeq ORF | 4017 bp |
| Locus ID | 5198 |
| UniProt ID | O15067 |
| Protein Pathways | Metabolic pathways, Purine metabolism |
| MW | 144.7 kDa |
| Gene Summary | Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217795 | PFAS (Myc-DDK-tagged)-Human phosphoribosylformylglycinamidine synthase (PFAS) |
CNY 13,728.00 |
|
| RC217795L3 | Lenti ORF clone of Human phosphoribosylformylglycinamidine synthase (PFAS), Myc-DDK-tagged |
CNY 15,200.00 |
|
| RC217795L4 | Lenti ORF clone of Human phosphoribosylformylglycinamidine synthase (PFAS), mGFP tagged |
CNY 15,200.00 |
|
| RG217795 | PFAS (tGFP-tagged) - Human phosphoribosylformylglycinamidine synthase (PFAS) |
CNY 15,328.00 |
