CEP250 (NM_007186) Human Untagged Clone

CAT#: SC303890

CEP250 (untagged)-Human centrosomal protein 250kDa (CEP250)



  "NM_007186" in other vectors (2)

CNY 47,030.00


货期*
13周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms C-NAP1; CEP2; CNAP1; CRDHL2
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_007186, the custom clone sequence may differ by one or more nucleotides
ATGGAGACAAGAAGCCCTGGGTTGAACAACATGAAGCCCCAGTCACTGCAGCTGGTACTG
GAAGAGCAGGTGCTGGCACTACAGCAGCAGATGGCAGAGAATCAGGCAGCCTCCTGGCGG
AAGCTGAAGAACTCCCAGGAGGCCCAGCAGAGACAAGCAACCCTTGTGAGGAAGCTGCAG
GCCAAGGTGCTGCAGTACCGAAGCTGGTGCCAAGAGCTGGAGAAGCGGCTAGAAGCCACT
GGAGGACCAATCCCCCAGAGGTGGGAAAATGTGGAGGAGCCAAACCTGGATGAGCTGCTG
GTCCGATTGGAGGAGGAGCAACAGAGGTGTGAGAGTCTAGCAGAGGTGAACACCCAGCTT
CGACTGCACATGGAAAAAGCTGACGTGGTGAATAAAGCCCTTAGGGAAGATGTGGAAAAA
CTGACAGTGGACTGGAGCCGGGCCCGGGATGAGCTAATGAGGAAGGAGAGCCAGTGGCAG
ATGGAGCAGGAGTTCTTCAAGGGCTACCTGAAAGGGGAGCACGGTCGCCTTCTCAGTCTA
TGGCGGGAGGTTGTGACATTCCGACGCCACTTCCTGGAAATGAAGTCAGCTACTGACAGA
GATCTGATGGAGCTAAAAGCTGAGCATGTGAGGCTTTCAGGGTCTCTGTTGACCTGTTGT
CTGCGCTTGACTGTGGGAGCACAGTCTCGGGAACCCAACGGATCTGGAAGAATGGATGGG
CGGGAGCCGGCCCAGCTGCTGCTGCTACTAGCCAAGACCCAGGAGCTGGAGAAGGAAGCC
CATGAAAGGAGCCAGGAGTTAATACAGCTGAAGAGTCAAGGGGATCTGGAGAAGGCTGAA
CTTCAGGACCGGGTGACCGAGCTCTCTGCTCTGTTGACCCAGTCTCAGAAGCAAAATGAA
GATTATGAAAAGATGATAAAGGCTCTGAGAGAGACAGTGGAGATCCTGGAGACAAATCAC
ACAGAATTAATGGAACATGAAGCATCTCTTAGTAGGAATGCGCAAGAGGAGAAGTTGTCT
TTACAGCAGGTGATCAAGGATATAACCCAGGTCATGGTGGAAGAAGGGGACAATATAGCC
CAAGGCTCTGGTCATGAGAACTCTTTGGAATTGGACTCTAGTATCTTCTCCCAGTTTGAT
TACCAGGATGCAGACAAGGCTCTTACTCTGGTGCGTTCAGTGCTGACTCGGAGACGCCAG
GCTGTGCAGGACCTAAGGCAGCAGCTTGCAGGCTGTCAAGAGGCTGTGAACTTGTTGCAA
CAGCAGCATGATCAGTGGGAGGAAGAGGGCAAAGCCTTGAGACAGCGGCTGCAGAAGCTC
ACTGGGGAGCGGGACACTCTGGCAGGGCAGACTGTGGACCTCCAGGGAGAGGTGGACTCT
CTCAGCAAGGAGCGAGAGCTGCTGCAGAAGGCCAGGGAAGAGCTGCGGCAGCAGCTGGAG
GTGCTAGAGCAGGAGGCATGGCGCCTGCGAAGGGTAAATGTGGAGCTTCAGCTGCAGGGG
GACTCTGCCCAGGGCCAGAAGGAGGAACAGCAGGAGGAGCTGCACCTGGCTGTCCGGGAG
AGGGAGCGTCTTCAGGAGATGCTGATGGGCCTGGAAGCCAAACAGTCAGAATCACTCAGT
GAACTGATCACTCTTCGGGAAGCCCTGGAGTCAAGTCACCTGGAAGGGGAGTTACTGAGG
CAAGAGCAAACGGAAGTGACCGCAGCGCTGGCTAGGGCAGAGCAGTCAATTGCAGAGCTG
TCGAGTTCTGAAAACACCCTGAAGACAGAAGTAGCTGATCTTCGGGCTGCAGCTGTCAAG
CTCAGTGCCTTAAATGAGGCTTTGGCGTTAGATAAAGTTGGGCTGAACCAGCAGCTTCTC
CAGTTAGAGGAGGAGAACCAGTCTGTGTGCAGCAGAATGGAGGCCGCAGAGCAGGCGAGA
AATGCTTTGCAGGTCGACCTGGCGGAGGCAGAGAAGAGGAGGGAAGCCCTGTGGGAAAAG
AACACTCACCTGGAGGCTCAGCTGCAGAAAGCTGAGGAGGCTGGGGCTGAGCTGCAGGCA
GATCTCAGGGACATCCAAGAAGAGAAGGAAGAAATTCAAAAGAAACTAAGTGAGTCACGT
CACCAGCAGGAGGCAGCCACGACTCAGCTGGAGCAGCTACATCAGGAGGCAAAGCGACAG
GAAGAAGTGCTTGCCAGGGCAGTCCAGGAGAAGGAGGCCCTAGTACGAGAGAAAGCGGCT
CTAGAGGTGCGGCTGCAGGCCGTGGAGCGTGACCGGCAGGACCTCGCTGAACAACTACAG
GGGCTCAGCTCAGCCAAGGAGCTACTGGAGAGCAGTCTGTTTGAAGCCCAACAACAAAAT
TCTGTGATAGAGGTCACCAAGGGGCAGCTGGAGGTCCAGATTCAAACTGTCACTCAAGCC
AAGGAAGTAATCCAAGGGGAAGTGAGGTGCCTGAAGCTGGAACTGGACACTGAACGGAGT
CAGGCAGAGCAGGAGCGGGATGCTGCAGCCAGACAGCTGGCCCAGGCTGAGCAAGAAGGG
AAGACTGCCTTGGAGCAGCAGAAGGCAGCCCATGAGAAAGAGGTGAACCAGCTCCGGGAG
AAATGGGAGAAGGAGCGCTCCTGGCACCAGCAGGAGCTGGCAAAGGCTCTGGAGAGCTTA
GAAAGGGAAAAAATGGAGCTGGAAATGAGGCTAAAGGAGCAGCAGACAGAAATGGAGGCC
ATCCAGGCCCAGAGGGAAGAAGAACGGACCCAGGCAGAGAGTGCCCTATGCCAGATGCAG
CTGGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGACACTGCTGCAGACGCAGAAGGAG
CTAGCAGATGCCAGCCAACAACTGGAACGACTGAGGCAGGACATGAAAGTCCAGAAATTA
AAGGAGCAGGAGACCACTGGGATACTACAGACCCAGCTCCAGGAGGCTCAACGGGAGCTG
AAGGAGGCAGCCCGGCAGCACAGAGATGACCTTGCTGCCCTCCAAGAAGAGAGCAGCTCC
CTGCTGCAGGATAAGATGGACCTGCAGAAGCAGGTGGAGGACTTGAAGTCTCAGCTGGTG
GCCCAGGATGACTCCCAGAGGCTGGTGGAGCAGGAGGTTCAGGAGAAGCTGAGAGAGACC
CAGGAGTATAACCGAATTCAGAAGGAGCTGGAGAGAGAGAAAGCCAGCCTGACTCTGTCA
CTGATGGAAAAGGAACAGAGACTCCTTGTTTTACAAGAAGCTGACTCTATTCGACAACAA
GAGCTGAGTGCCCTGCGCCAGGACATGCAGGAGGCCCAGGGAGAACAGAAAGAGCTCAGT
GCTCAGATGGAATTACTAAGGCAAGAGGTGAAGGAAAAGGAGGCTGACTTTCTGGCCCAG
GAAGCACAGCTGCTGGAGGAGCTGGAGGCGTCTCATATCACGGAGCAGCAGCTGCGAGCC
TCCTTGTGGGCCCAGGAAGCCAAGGCAGCCCAACTACAGCTGCGACTGCGCAGCACAGAG
AGCCAGCTAGAAGCGCTGGCCGCAGAGCAGCAGCCCGGGAACCAGGCCCAGGCCCAGGCC
CAGCTGGCCAGCCTCTACTCTGCCCTGCAGCAGGCCCTGGGGTCTGTTTGTGAGAGCAGG
CCTGAGCTGAGTGGTGGGGGAGACTCTGCTCCTTCCGTCTGGGGCCTTGAGCCAGACCAG
AATGGAGCTAGGAGCCTCTTTAAGAGAGGGCCCCTGCTGACTGCTCTCTCCGCTGAGGCA
GTAGCATCTGCCCTCCACAAGCTTCATCAAGACCTGTGGAAGACTCAACAGACCCGGGAT
GTTCTGAGGGATCAGGTCCAGAAACTGGAAGAGCGTCTAACTGATACTGAGGCTGAGAAG
AGCCAGGTCCACACAGAGTTGCAGGATCTGCAGAGACAGCTCTCCCAGAATCAGGAAGAG
AAATCCAAGTGGGAAGGAAAGCAGAACTCCCTAGAATCTGAGCTGATGGAACTACATGAA
ACTATGGCATCCTTACAGAGTCGCCTGCGGAGAGCAGAGCTACAGCGAATGGAAGCCCAG
GGTGAGCGAGAGTTACTTCAGGCAGCCAAGGAGAACCTGACAGCCCAGGTGGAACACCTG
CAAGCAGCTGTCGTAGAAGCCAGGGCTCAGGCAAGTGCTGCTGGCATCCTGGAAGAAGAC
CTGAGAACGGCTCGCTCAGCACTGAAGCTGAAAAATGAGGAAGTAGAGAGTGAGCGTGAG
AGAGCCCAGGCTCTGCAAGAGCAGGGCGAACTGAAGGTGGCCCAAGGGAAGGCTCTGCAA
GAGAATTTGGCCCTCCTGACCCAGACCCTAGCTGAAAGAGAAGAGGAGGTGGAGACTCTG
CGGGGACAAATCCAGGAACTGGAGAAGCAACGGGAAATGCAGAAGGCTGCTTTGGAATTG
CTGTCTCTGGACCTGAAGAAGAGGAACCAAGAGGTAGATCTGCAGCAAGAACAGATTCAG
GAGCTAGAGAAGTGTAGGTCTGTTTTAGAGCATCTGCCCATGGCCGTCCAGGAGCGAGAG
CAGAAGCTGACTGTGCAGAGGGAGCAGATCAGAGAGCTCGAGAAGGATCGGGAGACTCAG
AGGAACGTCTTGGAGCATCAGCTTCTAGAACTTGAGAAGAAAGACCAAATGATTGAGTCC
CAGAGAGGACAGGTTCAGGACCTGAAAAAGCAGTTGGTTACTCTGGAATGCCTGGCCCTG
GAACTGGAGGAAAACCATCACAAGATGGAGTGCCAGCAAAAACTGATCAAGGAGCTGGAG
GGCCAGAGGGAAACCCAGAGAGTGGCTTTGACCCACCTTACGCTGGACCTAGAAGAAAGG
AGCCAGGAGCTGCAGGCACAAAGCAGCCAGATCCATGACCTGGAGAGCCACAGCACCGTT
CTGGCAAGAGAGCTGCAGGAGAGGGACCAGGAGGTGAAGTCTCAGCGAGAACAGATCGAG
GAGCTGCAGAGGCAGAAAGAGCATCTGACTCAGGATCTCGAGAGGAGAGACCAGGAGCTG
ATGCTGCAGAAGGAGAGGATTCAGGTTCTCGAGGATCAGAGGACCCGGCAGACCAAGATC
CTGGAGGAGGACCTGGAACAGATCAAGCTGTCCTTGAGAGAGCGAGGCCGGGAGCTGACC
ACTCAGAGGCAGCTGATGCAGGAACGGGCAGAGGAAGGGAAGGGCCCAAGTAAAGCACAG
CGCGGGAGCCTAGAGCACATGAAGCTGATCCTGCGTGATAAGGAGAAGGAGGTGGAATGT
CAGCAGGAGCATATCCATGAACTCCAGGAGCTCAAAGACCAGCTGGAGCAGCAGCTCCAG
GGCCTGCACAGGAAGGTAGGTGAGACCAGCCTCCTCCTGTCCCAGCGAGAGCAGGAAATA
GTGGTCCTGCAGCAGCAACTGCAGGAAGCCAGGGAACAAGGGGAGCTGAAGGAGCAGTCA
CTTCAGAGTCAACTGGATGAGGCCCAGAGAGCCCTAGCCCAGAGGGACCAGGAACTGGAG
GCTCTGCAGCAAGAACAGCAGCAGGCCCAGGGACAGGAGGAGAGGGTGAAGGAAAAGGCA
GACGCCCTCCAGGGAGCTCTGGAGCAAGCCCATATGACACTGAAGGAGCGTCATGGAGAG
CTTCAGGACCACAAGGAACAGGCACGAAGGCTGGAGGAAGAGCTGGCAGTGGAGGGACGG
CGGGTCCAGGCCCTGGAGGAGGTGCTGGGAGACCTAAGGGCTGAGTCTCGGGAACAGGAG
AAAGCTCTGTTGGCCCTCCAGCAGCAGTGTGCTGAGCAGGCACAGGAGCATGAGGTGGAG
ACCAGGGCCCTGCAGGACAGCTGGCTGCAGGCCCAGGCAGTGCTCAAGGAACGGGACCAG
GAGCTGGAAGCTCTGCGGGCAGAAAGTCAGTCCTCCCGGCATCAGGAGGAGGCTGCCCGG
GCCCGGGCTGAGGCTCTGCAGGAGGCCCTTGGCAAGGCTCATGCTGCCCTGCAGGGGAAA
GAGCAGCATCTCCTCGAGCAGGCAGAATTGAGCCGCAGTCTGGAGGCCAGCACTGCAACC
CTGCAAGCCTCCCTGGATGCCTGCCAGGCACACAGTCGGCAGCTGGAGGAGGCTCTGAGG
ATACAAGAAGGTGAGATCCAGGACCAGGATCTCCGATACCAGGAGGATGTGCAGCAGCTG
CAGCAGGCACTTGCCCAGAGGGATGAAGAGCTGAGACATCAGCAGGAACGGGAGCAGCTG
CTGGAGAAGTCTCTGGCCCAGAGGGTCCAAGAGAATATGATCCAAGAGAAGCAGAATCTG
GGGCAAGAGAGAGAAGAGGAGGAGATAAGGGGCCTTCATCAGAGTGTAAGGGAGCTACAG
CTGACTCTAGCCCAAAAGGAACAGGAGATTCTGGAGCTGAGGGAGACCCAGCAAAGGAAC
AACCTGGAAGCCTTACCCCACAGCCACAAAACCTCCCCAATGGAGGAACAATCTCTAAAA
CTTGATTCTTTAGAGCCCAGGCTGCAGCGGGAGCTGGAGCGGCTACAGGCAGCCCTGAGA
CAGACAGAAGCCAGGGAGATTGAGTGGAGGGAGAAGGCCCAGGACTTGGCACTCTCCCTA
GCGCAGACCAAGGCCAGTGTCAGCAGTCTGCAGGAGGTAGCCATGTTCCTACAAGCCTCT
GTCCTGGAGCGGGACTCAGAACAGCAAAGGCTGCAGGATGAACTGGAGCTCACCAGACGG
GCTCTGGAGAAGGAGCGGCTACACAGCCCAGGTGCAACCAGCACAGCAGAACTGGGGTCC
AGAGGGGAGCAGGGTGTGCAGCTGGGAGAGGTCTCAGGAGTGGAGGCTGAGCCTAGTCCT
GATGGAATGGAGAAGCAGTCATGGAGACAAAGGCTTGAACACCTGCAGCAAGCAGTGGCC
CGGCTGGAGATTGACAGGAGCAGGCTGCAGCGCCACAATGTCCAGCTGCGGAGTACCTTG
GAGCAGGTGGAGCGAGAACGGAGGAAGCTGAAGAGGGAGGCCATGCGTGCGGCCCAGGCA
GGGTCCCTAGAGATCAGCAAGGCCACGGCTTCTTCACCCACACAGCAGGATGGGAGAGGA
CAGAAGAACTCAGATGCCAAGTGTGTGGCTGAACTGCAGAAAGAGGTGGTCCTGCTGCAA
GCTCAGCTGACTTTGGAGCGGAAGCAGAAGCAGGACTACATCACCCGCTCAGCACAGACC
AGCCGTGAGCTAGCAGGCCTGCACCACAGCCTCTCACACTCACTTCTTGCCGTGGCCCAG
GCCCCTGAGGCCACTGTCCTGGAGGCAGAGACCCGCAGGCTGGATGAGTCCCTGACTCAA
AGTCTGACATCCCCAGGGCCAGTCCTGCTACACCCCAGCCCCAGCACTACCCAAGCCGCC
TCCAGGTAG
Restriction Sites Please inquire     
ACCN NM_007186
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007186.2, NP_009117.2
RefSeq Size 8365 bp
RefSeq ORF 7329 bp
Locus ID 11190
UniProt ID Q9BV73
Gene Summary This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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