RP1 (NM_006269) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DCDC4A; ORP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303763 representing NM_006269. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTGATACCCCTTCTACTGGTTTTTCCATCATTCATCCTACGTCTTCTGAAGGTCAAGTTCCACCC CCTCGCCATTTGAGCCTCACTCATCCTGTTGTGGCCAAGCGAATCAGTTTCTACAAGAGCGGAGACCCC CAATTCGGCGGGGTCAGGGTGGTGGTCAACCCTCGCTCCTTTAAGTCCTTTGATGCTCTGCTGGATAAC TTGTCCAGGAAGGTGCCCCTCCCTTTTGGAGTGAGGAACATCAGCACCCCTCGGGGCAGGCACAGCATC ACGCGCCTGGAGGAGCTGGAGGACGGCGAGTCCTACCTATGTTCCCACGGCAGGAAGGTGCAGCCTGTA GACCTGGACAAAGCCCGTCGGCGCCCGCGGCCCTGGCTCAGCAGCCGGGCCATTAGCGCGCACTCACCG CCCCACCCCGTAGCCGTCGCTGCTCCCGGCATGCCCCGCCCCCCACGGAGCCTAGTGGTCTTCAGGAAT GGCGACCCGAAGACGAGGCGTGCGGTTCTTCTGAGCAGGAGGGTCACCCAGAGCTTCGAGGCATTTCTA CAGCACCTGACAGAGGTCATGCAGCGCCCTGTGGTCAAGCTGTACGCTACGGACGGAAGGAGGGTTCCC AGCCTCCAGGCAGTGATCCTGAGCTCTGGAGCTGTGGTGGCGGCAGGAAGGGAGCCATTTAAACCAGGA AATTATGACATCCAAAAATACTTGCTTCCTGCTAGATTACCAGGGATCTCTCAGCGTGTGTACCCCAAG GGAAATGCAAAGTCAGAAAGCAGAAAGATAAGCACACATATGTCTTCAAGCTCAAGGTCCCAGATTTAT TCTGTTTCTTCTGAGAAAACACATAATAATGATTGCTACTTAGACTATTCTTTTGTTCCTGAAAAGTAC TTGGCCTTAGAAAAGAATGATTCTCAGAATTTACCAATATATCCTTCTGAAGATGATATTGAGAAATCA ATTATTTTTAATCAAGACGGCACTATGACAGTTGAGATGAAAGTTCGATTCAGAATAAAAGAGGAAGAA ACCATAAAATGGACAACTACTGTCAGTAAAACTGGTCCTTCTAATAATGATGAAAAGAGTGAGATGAGT TTTCCAGGAAGAACAGAAAGTCGATCATCTGGTTTAAAGCTTGCAGCATGTTCATTCTCTGCAGATGTG TCACCTATGGAGCGAAGCAGTAATCAAGAGGGCAGTTTGGCAGAGGAGATAAACATTCAAATGACAGAT CAAGTGGCTGAAACTTGCAGTTCTGCTAGTTGGGAGAATGCTACTGTGGACACAGATATCATCCAGGGA ACTCAAGACCAAGCAAAGCATCGTTTTTATAGGCCCCCTACACCTGGACTAAGAAGAGTGAGACAAAAG AAATCTGTGATTGGCAGTGTGACCTTAGTATCTGAAACTGAGGTTCAAGAGAAAATGATTGGACAGTTT TCATATAGTGAAGAAAGGGAAAGTGGGGAAAACAAGTCTGAGTATCACATGTTTACACATTCTTGCAGT AAAATGTCATCAGTATCTAACAAACCAGTACTTGTTCAGATCAATAACAATGATCAAATGGAGGAGTCA TCATTAGAAAGAAAAAAGGAAAACAGTCTGCTTAAGTCAAGTGCAATAAGTGCTGGTGTTATAGAAATT ACAAGTCAGAAGATGTTAGAGATGTCACATAATAATGGTTTGCCATCAACTATATCAAATAACTCAATT GTGGAGGAAGATGTAGTTGATTGTGTGGTATTGGACAACAAAACTGGTATCAAGAACTTCAAAACTTAT GGTAACACCAATGATAGGTTCAGTCCTATTTCAGCAGATGCAACCCATTTTTCAAGTAATAACTCTGGA ACTGACAAAAATATTTCTGAGGCTCCAGCTTCAGAAGCATCCTCTACTGTCACTGCAAGAATTGACAGA CTAATTAATGAATTTGCTCAGTGTGGTTTAACAAAACTTCCAAAAAATGAAAAGAAGATTTTGTCATCT GTTGCCAGCAAAAAGAAGAAAAAATCTCGACAGCAAGCAATAAATTCCAGGTATCAAGATGGACAGCTT GCAACCAAAGGAATTCTTAATAAGAATGAGAGAATAAACACAAAAGGTAGAATTACAAAGGAAATGATA GTGCAAGATTCAGATAGTCCCCTTAAAGGAGGGATACTTTGTGAGGAAGACCTCCAGAAAAGTGATACT GTAATTGAATCAAATACTTTTTGTTCCAAAAGTAATCTCAATTCCACGATTTCCAAGAATTTCCATAGA AATAAATTAAATACTACTCAAAATTCCAAGGTTCAAGGACTTTTAACCAAAAGAAAATCTAGATCACTA AATAAAATAAGCTTAGGAGCACCTAAAAAAAGAGAAATCGGTCAAAGAGATAAAGTGTTTCCTCACAAT GAATCTAAATATTGCAAAAGTACTTTTGAAAACAAAAGTTTATTTCATGTATTTAACATCCTTGAGCAA AAACCCAAAGATTTTTATGCACCGCAATCTCAAGCAGAAGTGGCATCTGGGTATTTGAGAGGAATGGCA AAGAAGAGTTTAGTTTCAAAAGTTACTGATTCACACATAACTTTAAAAAGCCAGAAAAAACGTAAAGGG GATAAAGTGAAAGCAAGTGCTATTTTAAGTAAACAACATGCTACAACCAGGGCAAATTCTTTAGCTTCT TTGAAAAAACCTGATTTTCCTGAGGCTATTGCTCATCATTCAATTCAAAATTATATACAGAGTTGGTTG CAGAACATAAATCCATATCCAACTTTAAAGCCTATAAAATCAGCTCCAGTATGTAGAAATGAAACGAGT GTGGTAAATTGTAGCAATAATAGTTTTTCAGGGAATGATCCCCATACAAATTCTGGAAAAATAAGTAAT TTTGTTATGGAAAGTAATAAGCACATAACTAAAATTGCCGGTTTGACAGGAGATAATCTATGTAAAGAG GGAGATAAGTCTTTTATTGCCAATGACACTGGTGAAGAAGATCTCCATGAGACACAGGTTGGATCTCTG AATGATGCTTATTTGGTTCCCCTGCATGAACACTGTACTTTGTCACAGTCAGCTATTAATGATCATAAT ACTAAAAGTCATATAGCTGCTGAAAAATCAGGACCAGAGAAAAAACTTGTTTACCAGGAAATAAACCTA GCTAGAAAAAGGCAAAGTGTAGAGGCTGCCATTCAAGTAGATCCTATAGAAGAGGAAACTCCAAAAGAC CTCTTACCAGTCCTGATGCTTCACCAATTGCAAGCTTCAGTTCCTGGTATTCACAAGACTCAGAATGGA GTTGTTCAAATGCCAGGTTCACTTGCAGGTGTTCCCTTTCATTCTGCAATATGTAATTCATCCACTAAT CTCCTTCTAGCTTGGCTCTTGGTGCTAAACCTAAAGGGAAGTATGAATAGCTTCTGTCAAGTTGATGCT CACAAGGCTACCAACAAATCTTCAGAAACACTTGCATTGTTGGAGATTCTAAAGCACATAGCTATCACA GAGGAAGCTGATGACTTGAAAGCTGCTGTTGCCAATTTAGTGGAGTCAACTACAAGCCACTTTGGACTC AGTGAGAAAGAACAAGACATGGTTCCAATAGATCTTTCTGCAAATTGTTCCACGGTCAACATTCAGAGT GTTCCTAAGTGCAGTGAAAATGAAAGAACACAAGGAATCTCCTCTTTGGATGGAGGTTGCTCTGCCAGT GAGGCATGTGCCCCTGAAGTCTGTGTTTTGGAAGTGACTTGCTCTCCATGTGAGATGTGCACTGTAAAT AAGGCTTATTCTCCAAAAGAGACATGTAACCCCAGTGACACTTTTTTTCCTAGTGATGGTTATGGTGTG GATCAGACTTCTATGAATAAGGCTTGTTTCCTAGGAGAGGTCTGTTCACTTACTGATACTGTGTTTTCT GATAAGGCTTGTGCTCAAAAGGAGAACCATACCTATGAGGGAGCTTGCCCAATTGATGAGACCTACGTT CCTGTCAATGTCTGCAATACCATTGACTTTTTAAACTCCAAAGAAAACACATATACTGATAACTTGGAT TCAACTGAAGAGTTAGAAAGAGGTGATGACATTCAGAAAGATCTAAATATTTTGACAGACCCTGAATAT AAAAATGGATTTAATACATTGGTGTCACATCAAAATGTCAGTAATTTAAGCTCCTGTGGCCTTTGCCTA AGTGAAAAAGAAGCAGAACTTGATAAGAAACATAGTTCTCTAGATGATTTTGAAAATTGTTCACTAAGG AAGTTTCAGGATGAAAATGCATATACTTCCTTTGATATGGAAGAACCACGGACTTCTGAAGAACCAGGC TCAATAACCAACAGCATGACATCAAGTGAAAGAAACATTTCAGAATTGGAATCTTTTGAAGAATTAGAA AACCATGACACTGATATCTTTAATACAGTGGTAAATGGAGGAGAGCAAGCCACTGAAGAATTAATCCAA GAAGAGGTAGAGGCTAGTAAAACTTTAGAATTGATAGACATCTCTAGTAAGAATATTATGGAAGAAAAA AGAATGAACGGTATAATTTATGAAATAATCAGTAAGAGGCTGGCAACACCACCATCTTTAGATTTTTGC TATGATTCTAAGCAAAATAGTGAAAAGGAGACCAATGAAGGAGAAACTAAGATGGTAAAAATGATGGTG AAAACTATGGAAACTGGAAGTTATTCAGAGTCCTCTCCTGATTTAAAAAAATGCATCAAAAGTCCAGTG ACTTCTGATTGGTCAGACTATCGGCCTGACAGTGACAGTGAGCAGCCATATAAAACATCCAGTGATGAT CCCAATGACAGTGGCGAACTTACCCAAGAGAAAGAATATAACATAGGATTTGTTAAAAGGGCAATAGAA AAACTGTACGGTAAAGCAGATATTATCAAACCATCTTTTTTTCCTGGGTCTACCCGCAAATCTCAGGTT TGTCCTTATAATTCTGTGGAATTTCAGTGTTCCAGGAAAGCAAGTCTTTATGATTCTGAAGGGCAGTCA TTTGGCTCTTCTGAACAGGTATCTAGTAGTTCATCTATGTTGCAGGAATTCCAGGAGGAAAGACAAGAT AAGTGTGATGTTAGTGCTGTGAGGGACAATTATTGTAGGGGTGACATTGTAGAACCTGGTACAAAACAA AATGATGATAGCAGAATCCTCACAGACATAGAGGAAGGAGTACTGATTGACAAAGGCAAATGGCTTCTG AAAGAAAATCATTTGCTAAGGATGTCATCTGAAAATCCTGGCATGTGTGGCAATGCAGACACCACATCA GTGGACACCCTACTTGATAATAACAGCAGTGAGGTACCATATTCACATTTTGGTAATTTGGCCCCAGGC CCAACGATGGATGAACTCTCCTCTTCAGAACTCGAGGAACTGACTCAACCCCTTGAACTAAAATGCAAT TACTTTAACATGCCTCATGGTAGTGACTCAGAACCTTTTCATGAGGACTTGCTGGATGTTCGCAATGAA ACCTGTGCCAAGGAAAGAATAGCAAATCATCATACAGAGGAGAAGGGTAGTCATCAGTCAGAAAGAGTA TGCACATCTGTCACTCATTCCTTTATTTCTGCTGGTAACAAAGTCTACCCTGTCTCTGATGATGCTATT AAAAACCAACCATTGCCTGGCAGTAATATGATTCATGGTACACTTCAGGAAGCTGACTCTTTGGATAAA CTGTATGCTCTTTGTGGTCAACATTGCCCAATACTAACTGTTATTATCCAACCCATGAATGAGGAAGAC CGAGGATTTGCATATCGCAAAGAATCTGATATTGAAAATTTCTTGGGTTTTTATTTATGGATGAAAATA CACCCATATTTACTTCAGACAGACAAAAATGTGTTCAGGGAAGAGAACAATAAAGCAAGTATGAGACAA AATCTTATTGATAATGCCATTGGTGATATATTTGATCAGTTTTATTTCAGTAACACATTTGACTTGATG GGTAAAAGAAGAAAACAAAAAAGAATTAACTTCTTGGGGTTAGAGGAAGAAGGTAATTTAAAGAAATTT CAACCAGATTTGAAGGAAAGGTTTTGTATGAATTTCTTGCACACATCATTGTTAGTTGTGGGTAATGTG GATTCAAATACACAAGACCTCAGCGGTCAGACAAATGAAATCTTTAAAGCAGTCGATGAGAATAACAAC TTATTAAATAACAGATTCCAGGGCTCAAGAACAAATCTCAACCAAGTAGTAAGAGAAAATATCAACTGT CATTACTTCTTTGAAATGCTTGGTCAAGCTTGCCTCTTAGATATTTGCCAAGTTGAGACCTCCTTAAAT ATTAGCAACAGAAATATTTTAGAACTTTGTATGTTTGAGGGTGAAAATCTTTTCATTTGGGAAGAGGAA GACATATTAAATTTAACTGATCTTGAAAGCAGTAGAGAACAAGAAGATTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_006269
Insert Size	6471 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_006269.1
RefSeq Size	7100 bp
RefSeq ORF	6471 bp
Locus ID	6101
UniProt ID	P56715
Protein Families	Druggable Genome
MW	240.7 kDa
Gene Summary	This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein, another retinal-specific protein, play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels, this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. Transcript variants resulted from an alternative promoter and alternative splicings have been found, which overlap the current reference sequence and has several exons upstream and downstream of the current reference sequence. However, the biological validity and full-length nature of some variants cannot be determined at this time.[provided by RefSeq, Sep 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.