PLCD1 (NM_006225) Human Untagged Clone

CAT#: SC303752

PLCD1 (untagged)-Human phospholipase C, delta 1 (PLCD1), transcript variant 2



  "NM_006225" in other vectors (4)

CNY 5,544.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-PLCD1 Antibody - N-terminal region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms NDNC3; PLC-III
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_006225 edited
CCGCACTCCGCTCGGACCCCAGGCCGCCGGTGCTGTCGCTACTCAAGTGAGTCCCGCGGT
GCCCCTCCCGCCGCGCCGCCCGTCGGGCATGGACTCGGGCCGGGACTTCCTGACCCTGCA
CGGCCTACAGGATGATGAGGATCTACAGGCGCTGCTGAAGGGCAGCCAGCTCCTGAAGGT
GAAGTCCAGCTCATGGAGGAGAGAGCGCTTCTACAAGTTGCAGGAGGACTGCAAGACCAT
CTGGCAGGAGTCCCGCAAGGTCATGCGGACCCCGGAGTCCCAGCTGTTCTCCATCGAGGA
CATTCAGGAGGTGCGAATGGGGCACCGCACGGAGGGTCTGGAGAAGTTCGCCCGTGATGT
GCCCGAGGACCGCTGCTTCTCCATTGTCTTCAAGGACCAGCGCAATACACTAGACCTCAT
CGCCCCATCGCCAGCTGATGCCCAGCACTGGGTGCTGGGGCTGCACAAGATCATCCACCA
CTCAGGCTCCATGGACCAGCGTCAGAAGCTACAGCACTGGATTCACTCCTGCTTGCGAAA
AGCTGACAAAAACAAGGACAACAAGATGAGCTTCAAGGAGCTGCAGAACTTCCTGAAGGA
GCTCAACATCCAGGTGGACGACAGCTATGCCCGGAAGATCTTCAGGGAGTGTGACCACTC
CCAGACAGACTCCCTGGAGGACGAGGAGATTGAGGCCTTCTACAAGATGCTGACCCAGCG
GGTGGAGATCGACCGCACCTTCGCCGAGGCCGCGGGCTCAGGGGAGACTCTGTCGGTGGA
TCAGTTAGTGACGTTCCTGCAGCACCAGCAGCGGGAGGAGGCGGCAGGGCCTGCGCTGGC
CCTCTCCCTCATTGAGCGCTACGAGCCCAGCGAGACTGCCAAGGCGCAGCGGCAGATGAC
CAAGGACGGCTTCCTCATGTACTTACTGTCGGCTGACGGCAGCGCCTTCAGCCTGGCACA
CCGCCGTGTCTACCAGGACATGGGCCAGCCACTTAGCCACTACCTGGTGTCCTCTTCACA
CAACACCTACCTGCTGGAGGACCAGCTAGCCGGGCCCAGCAGCACTGAAGCCTACATCCG
GGCACTGTGCAAAGGCTGCCGATGCCTGGAGCTTGACTGCTGGGACGGGCCCAACCAGGA
ACCAATCATCTACCACGGCTATACTTTCACTTCCAAGATCCTCTTCTGCGATGTGCTCAG
GGCCATCCGGGACTATGCCTTCAAGGCGTCCCCCTACCCTGTCATCCTATCCCTGGAGAA
CCACTGCACACTGGAGCAGCAGCGCGTGATGGCGCGGCACCTGCATGCCATCCTGGGCCC
CATGCTGTTGAACCGACCACTGGATGGGGTCACCAACAGCCTGCCCTCCCCTGAGCAACT
GAAGGGGAAGATCCTGCTGAAGGGGAAGAAGCTCGGGGGGCTCCTGCCCCCTGGAGGGGA
GGGTGGCCCTGAGGCCACTGTGGTGTCAGACGAAGACGAGGCTGCTGAGATGGAGGATGA
GGCAGTGAGGAGCCGTGTGCAGCACAAGCCCAAGGAGGACAAGCTCAGGCTAGCACAGGA
GCTCTCTGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGGGGCTTCTCCAGTCCTGG
CACCCCTGGACAGGCCTTCTACGAGATGGCGTCCTTCTCTGAGAACCGTGCCCTTCGACT
GCTCCAAGAATCAGGAAACGGCTTTGTCCGCCACAACGTGGGGCACCTGAGCAGAATCTA
CCCGGCTGGATGGAGAACAGACTCCTCCAACTACAGCCCCGTGGAGATGTGGAATGGGGG
CTGCCAGATCGTGGCCCTGAATTTCCAGACACCTGGGCCAGAGATGGACGTGTACCAGGG
CCGCTTCCAGGACAACGGGGCCTGTGGGTACGTGCTGAAGCCCGCCTTCCTGCGAGACCC
CAACGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCACGGAAGCGGCT
CAACATCAGGGTCATTTCGGGGCAGCAGCTGCCAAAAGTCAACAAGAATAAGAATTCAAT
TGTGGACCCCAAAGTGACAGTGGAGATCCATGGCGTGAGCCGGGACGTGGCCAGCCGCCA
GACTGCTGTCATCACCAACAATGGTTTCAACCCATGGTGGGACACGGAGTTTGCGTTTGA
GGTAGTTGTGCCTGACCTTGCCCTCATCCGCTTCTTGGTGGAAGATTATGATGCCTCCTC
CAAGAATGACTTCATTGGCCAGAGTACCATCCCCTTGAACAGCCTCAAGCAAGGATACCG
CCATGTCCACCTCATGTCTAAGAACGGGGACCAGCATCCATCAGCCACCCTCTTTGTGAA
GATCTCCCTCCAGGACTAGGCTGGAGGAAGCCAGTGGGGTCCCCCCTGAGTGGGCTGGGC
CCTCTGTCCACATGTGGGGACAGGGCTGGTGTGGCTGCTCCCAGCCTCTTGCTCAGAGCT
AGGCCCCCAAATTGCCTTCAGCCCTAACATAGTGTCTGCTGCTGCCTCCCTGGGGACCAG
GAGCTAGCCCAGTCCCTGGAGCTGTCCTTCATTCCGTTAGGAATAACACTGCAGCCCTCT
CCACCCTCCGGCCAGCGAGTGGTCAAGGATTTTTATAAAAATCACGATAAGATTAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_006225
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006225.1, NP_006216.1
RefSeq Size 2627 bp
RefSeq ORF 2271 bp
Locus ID 5333
UniProt ID P51178
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
Gene Summary This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to transcript variant 1, and initiates translation from an in-frame upstream AUG, resulting in a shorter isoform (2) with a different N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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