Myosin Heavy chain 1 (MYH1) (NM_005963) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303712 representing NM_005963. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTTCCGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAGGGAG CGAATTGAAGCCCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCC TTTGTGAAAGCAACAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCTAAGACCGAAGCTGGAGCTACT GTAACAGTGAAAGATGACCAAGTCTTCCCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCC ATGATGACTCATCTACACGAGCCTGCTGTGCTGTACAACCTCAAAGAGCGCTACGCAGCCTGGATGATC TACACCTACTCAGGCTTGTTCTGTGTCACTGTCAACCCCTACAAGTGGTTGCCAGTGTATAATGCAGAG GTGGTGACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAAT GCCTATCAGTTCATGCTGACTGATCGGGAGAATCAGTCTATCTTGATCACCGGAGAATCTGGCGCAGGG AAGACTGTGAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGGGAGAAGAAGAAG GAAGAAGTTACTTCTGGCAAAATGCAGGGGACTCTGGAAGATCAAATCATCAGTGCCAACCCCCTACTG GAGGCCTTTGGCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATC CACTTCGGTACCACAGGGAAACTGGCTTCTGCTGATATTGAAACATATCTTCTGGAGAAGTCTAGAGTT ACTTTCCAGCTAAAGGCTGAAAGAAGCTATCATATTTTTTATCAGATCATGTCTAACAAGAAGCCAGAT CTAATTGAAATGCTCCTGATCACCACCAACCCATACGATTATGCCTTCGTCAGTCAAGGGGAGATCACA GTGCCCAGCATTGATGACCAAGAAGAGTTGATGGCTACAGATAGTGCCATTGAAATTCTGGGCTTTACT TCAGATGAAAGAGTGTCCATCTATAAGCTCACAGGGGCTGTGATGCATTATGGGAACATGAAATTCAAG CAAAAGCAGCGTGAGGAGCAAGCTGAGCCAGATGGCACTGAAGTTGCTGACAAGGCAGCCTATCTCCAA AATCTGAACTCTGCAGATCTGCTCAAAGCCCTCTGCTACCCTAGGGTCAAGGTCGGCAATGAGTATGTC ACCAAAGGTCAAACTGTGCAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCTGTCTACGATAAG ATGTTCTTGTGGATGGTCACCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATT GGGGTCTTGGACATTGCTGGCTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTC ACCAATGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAG GAAGGCATTGAGTGGACGTTCATTGACTTTGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAG CCTATGGGCATCTTCTCCATCCTGGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAG AACAAGCTGTATGAACAACATCTTGGAAAATCCAATAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAG CCTGAGGCCCACTTCTCTTTGATTCACTATGCTGGCACCGTGGACTACAACATTGCCGGCTGGCTTGAC AAGAACAAGGACCCCCTGAATGAGACTGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCT CTCCTCTTTGTTGGGGCAACGGGAGCGGAAGCAGAGGCTGGCGGTGGAAAGAAAGGTGGTAAGAAGAAG GGTTCTTCTTTCCAGACTGTGTCTGCTCTCTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGG AGCACTCACCCCCACTTTGTGCGGTGCATCATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCAT GAGCTTGTCCTGCATCAGCTGAGGTGTAACGGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTC CCAAGCAGAATCCTTTATGCAGACTTCAAACAGAGATACAAGGTGTTAAATGCAAGTGCTATCCCTGAA GGACAATTCATCGATAGCAAGAAGGCTTCAGAGAAGCTCCTGGGGTCCATTGACATTGACCACACCCAG TATAAATTTGGTCACACCAAGGTCTTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGCGAGAT GAGAAGCTGGCCCAGCTGATTACCCGAACCCAGGCCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTAC CAGAAAATGGTGGAAAGAAGAGAGTCCATCTTCTGCATCCAGTACAATGTCCGTGCCTTCATGAATGTG AAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATCAAACCCCTCCTCAAAAGTGCAGAGACAGAGAAG GAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCAAAGAAGAGCTGGCTAAGACCGAGGCAAAAAGG AAAGAGCTGGAAGAAAAAATGGTTACTCTGATGCAAGAAAAAAATGACTTGCAACTCCAGGTTCAAGCT GAAGCTGACAGCTTGGCTGATGCAGAGGAAAGGTGTGACCAGCTAATCAAAACCAAAATCCAGCTAGAA GCCAAAATCAAAGAGGTGACTGAGAGAGCTGAGGATGAGGAAGAGATCAATGCTGAGCTGACAGCCAAG AAGAGGAAACTGGAGGATGAATGTTCAGAACTCAAGAAAGACATTGATGACCTTGAGCTGACACTGGCC AAGGTTGAGAAGGAGAAACATGCCACAGAAAACAAGGTGAAAAACCTCACAGAAGAGATGGCGGGTCTG GATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCCAGGAGGCCCACCAGCAGACCCTGGAT GACCTGCAGGCAGAGGAGGACAAAGTCAACACCCTGACCAAAGCTAAAATCAAACTTGAACAACAAGTG GATGATCTTGAAGGATCTTTGGAACAAGAAAAGAAAATCCGGATGGATCTAGAAAGAGCAAAGAGAAAA CTAGAGGGAGACCTAAAATTGGCTCAAGAATCCACAATGGATATAGAAAATGACAAACAACAACTTGAT GAAAAGCTTAAAAAGAAAGAGTTTGAAATGAGCGGTCTGCAAAGCAAGATTGAAGATGAACAAGCCCTT GGTATGCAGCTGCAGAAGAAAATCAAGGAGTTACAAGCCCGCATTGAGGAGCTGGAGGAGGAAATCGAG GCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGATCTCTCCCGGGAGCTGGAGGAGATC AGCGAGAGGCTGGAAGAAGCCGGTGGGGCCACCTCAGCCCAGATTGAGATGAACAAGAAGCGGGAGGCT GAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTACAGCATGAAGCCACGGCGGCCACCCTG AGGAAGAAGCATGCAGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGAGTGAAGCAG AAGCTGGAGAAGGAGAAGAGTGAGATGAAGATGGAGATCGATGACCTTGCTAGTAACATGGAGACTGTC TCCAAAGCCAAGGGAAACCTTGAAAAGATGTGCCGCGCTCTAGAAGATCAACTGAGTGAAATTAAGACC AAGGAAGAGGAGCAGCAGCGGCTGATCAATGACCTCACAGCACAGAGAGCGCGCCTGCAAACAGAATCA GGTGAATATTCACGCCAGCTAGATGAAAAGGACACACTAGTTTCACAGCTCTCGAGGGGCAAACAAGCC TTTACACAACAGATTGAGGAACTGAAAAGGCAACTTGAAGAGGAGATAAAGGCCAAGAGTGCCCTGGCA CATGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAGCC AAGGCCGAGCTACAGAGAGCAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGACCAAATATGAG ACAGATGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTGGCTCAGCGTCTGCAGGAT GCTGAGGAACATGTAGAAGCTGTGAATGCCAAATGTGCTTCCCTTGAGAAGACGAAGCAGAGGCTCCAG AATGAAGTTGAGGACCTCATGATTGATGTTGAGAGGACAAATGCTGCCTGTGCCGCCCTGGACAAAAAG CAAAGGAACTTTGATAAGATCCTGGCAGAATGGAAACAGAAGTGTGAAGAAACTCATGCTGAACTTGAA GCTTCTCAAAAGGAATCCCGCTCACTCAGCACAGAACTATTTAAGATTAAGAATGCTTATGAGGAATCT TTAGACCAACTTGAAACCTTGAAACGGGAAAATAAGAATTTGCAACAGGAGATTTCTGATCTCACTGAA CAGATTGCAGAAGGAGGAAAGCGCATCCATGAACTGGAAAAAATAAAGAAGCAAGTTGAGCAAGAAAAG TCTGAACTTCAGGCTGCCTTAGAGGAGGCAGAGGCATCTCTTGAACATGAAGAGGGAAAGATCCTGCGC ATCCAGCTTGAGTTGAACCAAGTCAAGTCTGAGGTTGATAGGAAAATTGCTGAAAAAGATGAGGAAATT GACCAGATGAAGAGAAACCACATTAGAATCGTGGAGTCCATGCAGAGCACACTGGATGCTGAGATCAGG AGCAGGAATGATGCCATTAGGCTCAAGAAGAAGATGGAGGGAGACCTCAATGAAATGGAAATCCAGCTG AACCATGCCAACCGCATGGCTGCTGAGGCCCTGAGGAACTATAGGAACACCCAAGCCATCCTCAAGGAT ACCCAGCTCCACCTGGATGATGCTCTCCGGAGCCAGGAGGACCTGAAGGAACAGCTGGCTATGGTGGAG CGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAACAGACAGAGAGGAGC AGGAAAATCGCAGAACAGGAGCTCCTGGATGCCAGTGAGCGTGTTCAGCTCCTGCACACCCAGAACACC AGCCTGATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAAGACATC ATCCAGGAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAG GAGCTGAAGAAGGAACAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACCTGGAACAGACGGTG AAGGACCTGCAGCATCGTCTGGATGAGGCTGAGCAGCTGGCCCTGAAGGGTGGGAAGAAGCAGATCCAG AAACTGGAGGCCAGGGTTCGTGAACTTGAAGGTGAAGTTGAAAGTGAACAGAAGCGCAATGTTGAAGCT GTCAAGGGTCTACGCAAACATGAGAGAAAAGTGAAGGAACTCACTTACCAAACTGAGGAAGACCGCAAG AATATTCTCAGGCTCCAGGACCTGGTGGACAAACTGCAAGCAAAGGTGAAATCCTACAAGAGACAAGCT GAAGAAGCGGAGGAACAATCCAACGTCAACCTCTCCAAATTCCGCAGGATCCAGCACGAGCTGGAGGAG GCCGAGGAACGGGCTGACATTGCTGAGTCCCAGGTCAACAAGCTGAGGGTGAAGAGCAGGGAGGTTCAC ACAAAAATCATAAGTGAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_005963
Insert Size	5820 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_005963.3
RefSeq Size	6038 bp
RefSeq ORF	5820 bp
Locus ID	4619
UniProt ID	P12882
Protein Pathways	Tight junction, Viral myocarditis
MW	223.1 kDa
Gene Summary	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]

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