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APOBEC3B (NM_004900) Human Untagged Clone
CAT＃: SC303543
APOBEC3B (untagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B)
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A3B; APOBEC1L; ARCD3; ARP4; bK150C2.2; DJ742C19.2; PHRBNL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004900 edited
CAAAAAAAGAGCGGGACAGGGACAAGCGTATCTAAGAGGCTGAACATGAATCCACAGATC AGAAATCCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAACGAACCC ATCCTCTATGGTCGGAGCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGC TCAAATCTCCTTTGGGACACAGGGGTCTTTCGAGGCCAGGTGTATTTCAAGCCTCAGTAC CACGCAGAAATGTGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGT TTCCAGATCACCTGGTTTGTATCCTGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCC GAATTCCTGTCTGAGCACCCCAATGTCACCCTGACCATCTCTGCCGCCCGCCTCTACTAC TACTGGGAAAGAGATTACCGAAGGGCGCTCTGCAGGCTGAGTCAGGCAGGAGCCCGCGTG ACGATCATGGACTATGAAGAATTTGCATACTGCTGGGAAAACTTTGTGTACAATGAAGGT CAGCAATTCATGCCTTGGTACAAATTCGATGAAAATTATGCATTCCTGCACCGCACGCTA AAGGAGATTCTCAGATACCTGATGGATCCAGACACATTCACTTTCAACTTTAATAATGAC CCTTTGGTCCTTCGACGGCGCCAGACCTACTTGTGCTATGAGGTGGAGCGCCTGGACAAT GGCACCTGGGTCCTGATGGACCAGCACATGGGCTTTCTATGCAACGAGGCTAAGAATCTT CTCTGTGGCTTTTACGGCCGCCATGCGGAGCTGCGCTTCTTGGACCTGGTTCCTTCTTTG CAGTTGGACCCGGCCCAGATCTACAGGGTCACTTGGTTCATCTCCTGGAGCCCCTGCTTC TCCTGGGGCTGTGCCGGGGAAGTGCGTGCGTTCCTTCAGGAGAACACACACGTGAGACTG CGCATCTTCGCTGCCCGCATCTATGATTACGACCCCCTATATAAGGAGGCGCTGCAAATG CTGCGGGATGCTGGGGCCCAAGTCTCCATCATGACCTACGATGAGTTTGAGTACTGCTGG GACACCTTTGTGTACCGCCAGGGATGTCCCTTCCAGCCCTGGGATGGACTAGAGGAGCAC AGCCAAGCCCTGAGTGGGAGGCTGCGGGCCATTCTCCAGGTGAGGGCTTCCTCCCTCTGC CTGGTGCCCCATCGGCCTCCCCCTCCTCCCCGCTCCCCTGTGCCTTGCCTTCCCCTCTGC TCAGAGCCTCCTCTGGGTTCCCTGCTCCCCACAGGGCGCCCAGCTCCGTCCCTCCCTTTC CTTCTCACAGCCTCCTTCTCTTTCCCACCTCCCGCATCCCTCCCTCCTCTCCCGTCATTG TCACTGTCCCCAGGCCACCTCCCTGTGCCCTCTTTCCACTCTCTCACCTCCTGCTCCATT CAACCCCCCTGCTCTTCCAGAATCAGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAA GGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGATCTTCTTCCAAGAAATGCAAACAGAC CGTTCACCACCATCTCCAGCTGCTCACAGACACCAGCAAAGCAATGTGCTCCTGATCAAG TAGATTTTTTAAAAATCAGAGTCAATTAATTTTAATTGAAAATTTCTCTTATGTTCCAAG TGTACAAGAGTAAGATTATGCTCAATATTCCCAGAATAGTTTTCAATGTATTAATGAAGT GATTAATTGGCTCCATATTTAGACTAATAAAACATTAAGAATCTTCCATAA |
| Restriction Sites | Please inquire |
| ACCN | NM_004900 |
| Insert Size | 1400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be 108 amino acids longer than NM_004900.3 at the 3'. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004900.3, NP_004891.3 |
| RefSeq Size | 1536 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 9582 |
| UniProt ID | Q9UH17 |
| Gene Summary | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. A hybrid gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3B, and the adjacent gene APOBEC3A. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Characterization of the Catalytic Domain of Human APOBEC3B and the Critical Structural Role for a Conserved Methionine
,Siriwardena, SU;Guruge, TA;Bhagwat, AS;,
J. Mol. Biol.
,PubMed ID 26281709
[APOBEC3B]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222712 | APOBEC3B (Myc-DDK-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) |
CNY 3,656.00 |
|
| RC222712L1 | Lenti-ORF clone of APOBEC3B (Myc-DDK-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) |
CNY 6,056.00 |
|
| RC222712L2 | Lenti-ORF clone of APOBEC3B (mGFP-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) |
CNY 5,890.00 |
|
| RC222712L3 | Lenti-ORF clone of APOBEC3B (Myc-DDK-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) |
CNY 6,056.00 |
|
| RC222712L4 | Lenti-ORF clone of APOBEC3B (mGFP-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) |
CNY 5,890.00 |
|
| RG222712 | APOBEC3B (tGFP-tagged) - Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) |
CNY 5,256.00 |
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