H3FJ (HIST1H3J) (NM_003535) Human Untagged Clone

CAT#: SC303330

HIST1H3J (untagged)-Human histone cluster 1, H3j (HIST1H3J)



  "NM_003535" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3FJ; HIST1H3J
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC303330 representing NM_003535.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCCGGACGAAGCAGACAGCTCGCAAGTCTACCGGCGGCAAGGCACCGCGGAAGCAGCTGGCCACC
AAGGCAGCGCGCAAAAGCGCTCCAGCGACTGGCGGTGTGAAGAAGCCCCACCGCTACAGGCCAGGCACC
GTGGCCTTGCGTGAGATCCGCCGTTATCAGAAGTCGACTGAGCTGCTCATCCGCAAACTGCCATTTCAG
CGCCTGGTGCGAGAAATCGCGCAGGATTTCAAAACCGACCTTCGTTTCCAGAGCTCGGCGGTGATGGCG
CTGCAAGAGGCGTGCGAGGCCTATCTGGTGGGTCTCTTTGAAGACACCAACCTCTGTGCTATTCACGCC
AAGCGTGTCACTATTATGCCTAAGGACATCCAGCTTGCGCGTCGTATCCGTGGCGAGCGAGCATAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_003535
Insert Size 411 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003535.2
RefSeq Size 478 bp
RefSeq ORF 411 bp
Locus ID 8356
UniProt ID P68431
Protein Pathways Systemic lupus erythematosus
MW 15.4 kDa
Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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