HRC (NM_002152) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	PCMV6-Neo
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_002152 edited CTCCGGAGACAGCCTCAGAGGGGTGTCCAGAGCCAGGCAGCAAGGACCAGAGGAAGGTGG ACAGAGACCGAAAGGACAAAGGAGAGACACAGAGAAAAAAAGAGACCAACCCCAAAGGGA CAAAGACCCCAACGTTTGTCCACATTGCCGCAGAGGCAGCTGGAGCCAGTGCTGCCTGTC CGTCCCCATGGGCCACCATAGGCCATGGCTGCACGCTTCTGTCCTCTGGGCTGGGGTGGC CAGCCTGCTCCTCCCCCCGGCCATGACCCAGCAGCTCAGAGGGGATGGGCTAGGCTTCAG AAACCGGAACAACAGCACTGGAGTCGCCGGGCTCTCCGAGGAGGCATCAGCAGAGCTTCG CCACCACCTCCACAGCCCTAGAGACCATCCAGATGAGAACAAGGATGTTTCCACAGAGAA TGGGCATCATTTCTGGAGCCACCCAGACCGTGAAAAGGAGGATGAAGATGTCTCCAAGGA ATATGGGCACCTACTCCCAGGCCACAGGTCCCAAGACCACAAAGTCGGAGATGAGGGTGT CTCAGGTGAGGAGGTCTTTGCAGAGCATGGTGGGCAGGCCCGTGGGCACAGAGGCCACGG GAGTGAAGACACGGAAGACTCAGCTGAGCACAGGCACCACCTCCCCAGCCACAGGAGCCA CAGCCATCAAGACGAGGATGAGGATGAAGTTGTGTCCAGTGAGCATCACCATCATATCCT CAGGCATGGACACCGAGGCCATGATGGGGAAGATGATGAAGGAGAAGAGGAGGAGGAGGA GGAGGAGGAGGAAGAGGAGGCCTCCACTGAGTATGGACACCAGGCCCACAGGCACCGAGG CCATGGGAGTGAAGAGGATGAGGATGTCTCAGATGGACACCATCATCATGGCCCCAGCCA CAGGCACCAAGGCCATGAAGAAGATGACGATGATGATGATGATGATGATGATGATGATGA TGATGATGATGTCTCCATTGAATATAGACACCAGGCTCACAGGCACCAAGGCCACGGGAT TGAAGAGGATGAAGATGTCTCAGATGGACACCATCATCGCGACCCCAGCCACAGGCACCG AAGCCATGAAGAAGATGACAATGATGATGATGATGTCTCCACTGAGTATGGACACCAGGC CCACAGGCACCAAGACCACAGAAAGGAAGAGGTTGAGGCTGTCTCAGGTGAACACCACCA TCATGTCCCTGACCACAGGCACCAAGGCCACAGAGACGAGGAAGAAGATGAGGATGTGTC CACTGAACGTTGGCACCAGGGTCCCCAACATGTCCACCATGGCCTTGTAGATGAGGAAGA GGAAGAAGAGGAGATCACAGTCCAGTTCGGCCACTATGTTGCAAGCCACCAACCTCGAGG CCACAAGAGTGATGAAGAGGACTTCCAAGATGAGTATAAAACAGAAGTCCCTCACCATCA CCACCACAGAGTCCCCAGGGAGGAAGATGAGGAGGTCTCTGCTGAGCTTGGCCACCAGGC CCCCAGCCACAGGCAAAGCCACCAAGATGAAGAAACTGGCCATGGTCAAAGAGGGTCCAT CAAAGAGATGAGCCATCACCCCCCAGGACACACAGTGGTCAAGGATAGAAGCCATTTGAG GAAGGATGATTCTGAGGAGGAAAAGGAGAAGGAAGAGGACCCCGGCTCCCATGAGGAAGA CGATGAAAGTTCAGAGCAGGGAGAGAAAGGCACCCATCATGGCAGCCGGGACCAGGAAGA TGAGGAGGATGAGGAGGAAGGTCATGGCCTCAGCCTGAACCAGGAGGAGGAAGAAGAGGA AGACAAGGAGGAGGAGGAGGAGGAAGAAGACGAGGAGAGGAGGGAAGAGAGGGCTGAGGT TGGGGCCCCACTGAGCCCAGACCACAGCGAGGAGGAAGAGGAGGAGGAGGAGGGGCTGGA GGAAGATGAGCCCCGCTTCACCATCATCCCCAACCCACTGGACAGGAGAGAGGAGGCTGG AGGTGCCTCCAGCGAGGAGGAAAGCGGTGAGGACACAGGTCCACAGGATGCTCAGGAGTA TGGGAACTACCAGCCAGGGTCCCTGTGTGGCTACTGCTCCTTCTGCAATCGATGCACTGA ATGTGAGAGCTGTCACTGTGATGAGGAGAACATGGGTGAGCACTGCGACCAGTGCCAGCA CTGTCAGTTCTGCTATCTCTGCCCGCTGGTCTGCGAAACGGTCTGCGCTCCAGGAAGCTA CGTTGACTATTTCTCCTCGTCCCTTTATCAGGCCCTGGCAGACATGCTGGAAACGCCGGA ACCCTGACCCAGTCGCTCGACTGCGACGCAGGTGTACCTCCCCCTGCCTTCCAACCCCTT TCCACGAGCTATATTTATTTCTCCGAATAAACGTGCTCCCCGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	Please inquire
ACCN	NM_002152
Insert Size	2400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_002152.2, NP_002143.1
RefSeq Size	2430 bp
RefSeq ORF	2100 bp
Locus ID	3270
UniProt ID	P23327
Gene Summary	This gene encodes a luminal sarcoplasmic reticulum protein identified by its ability to bind low-density lipoprotein with high affinity. The protein interacts with the cytoplasmic domain of triadin, the main transmembrane protein of the junctional sarcoplasmic reticulum (SR) of skeletal muscle. The protein functions in the regulation of releasable calcium into the SR. [provided by RefSeq, Sep 2008]

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