United States
Germany
Japan
United Kingdom
China
Histidase (HAL) (NM_002108) Human Untagged Clone
CAT＃: SC303122
HAL (untagged)-Human histidine ammonia-lyase (HAL)
CNY 7,220.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HIS; HSTD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303122 representing NM_002108.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCAGATACACGGTGCACGTACGTGGGGAATGGCTGGCAGTGCCCTGCCAGGACGCGCAGCTCACT GTGGGCTGGCTGGGCCGGGAGGCCGTGAGGCGCTATATCAAGAATAAGCCCGACAATGGTGGCTTCACC TCCGTGGATGACGCGCACTTCCTTGTGCGCCGGTGCAAGGGCCTGGGCCTGCTGGACAACGAGGACCGG CTCGAGGTGGCCCTAGAGAACAACGAGTTCGTGGAAGTGGTTATAGAGGGTGATGCCATGTCTCCTGAC TTCATTCCATCTCAACCAGAAGGAGTTTATCTATACAGCAAGTACCGGGAGCCTGAAAAGTACATCGAG TTAGATGGAGACCGTCTGACCACGGAGGATCTGGTCAACTTGGGAAAGGGACGCTACAAAATAAAGCTC ACCCCAACAGCTGAGAAGAGGGTGCAGAAATCCAGGGAGGTCATAGATAGCATCATAAAAGAGAAAACA GTTGTTTACGGTATTACTACAGGTTTTGGGAAATTTGCCAGAACTGTAATTCCTATCAATAAGCTACAG GAGCTTCAGGTCAACTTAGTACGCTCACATTCTTCAGGTGTTGGGAAACCACTAAGTCCTGAGAGGTGT CGGATGCTCTTGGCTTTAAGGATCAATGTCTTAGCCAAAGGATACAGTGGCATTTCCCTGGAGACCCTC AAACAAGTCATAGAAATGTTTAATGCCTCCTGCCTGCCCTATGTCCCAGAGAAAGGAACCGTTGGTGCC AGTGGAGACCTTGCCCCACTCTCTCATCTTGCTCTTGGGCTAGTTGGAGAAGGGAAGATGTGGTCTCCG AAGAGTGGCTGGGCTGATGCTAAATACGTGCTAGAAGCCCATGGATTGAAACCAGTTATTTTAAAACCA AAAGAGGGCCTGGCACTCATCAATGGGACGCAGATGATCACATCCCTGGGCTGTGAAGCTGTAGAGCGA GCCAGTGCTATTGCACGGCAGGCTGACATTGTGGCAGCCCTGACCCTTGAGGTGCTGAAGGGCACCACC AAAGCCTTTGACACTGACATTCATGCTCTTCGACCTCACCGTGGGCAAATTGAAGTTGCTTTTCGGTTT CGGTCACTCTTGGACTCAGATCACCACCCATCAGAAATAGCAGAGAGTCACAGGTTCTGTGATCGCGTC CAGGATGCATACACCTTGCGCTGCTGTCCACAGGTCCATGGTGTGGTGAATGATACAATAGCATTTGTG AAGAACATCATTACCACAGAACTGAACAGCGCAACAGATAATCCTATGGTCTTTGCCAATAGGGGAGAG ACAGTTTCTGGAGGAAACTTCCATGGTGAATACCCAGCCAAAGCCCTAGACTACTTGGCCATTGGCATC CATGAACTTGCTGCAATCAGTGAGAGAAGAATCGAGCGGCTCTGCAATCCCTCCCTCAGTGAGCTGCCT GCCTTCCTGGTGGCTGAAGGTGGTCTGAACTCTGGGTTCATGATAGCTCACTGCACGGCAGCAGCCCTT GTTTCTGAGAACAAGGCTCTGTGCCATCCCTCGTCTGTTGACTCCCTCTCCACCAGCGCAGCCACGGAG GACCACGTCTCCATGGGAGGATGGGCAGCAAGGAAAGCCCTCAGGGTCATCGAGCATGTGGAGCAAGTG CTGGCCATCGAGCTCCTTGCAGCCTGCCAGGGCATAGAGTTTCTACGTCCCCTGAAAACAACCACTCCG CTGGAGAAGGTCTATGACCTGGTGCGCTCTGTTGTAAGGCCCTGGATAAAAGATCGCTTCATGGCCCCG GACATCGAGGCAGCCCACAGGCTGCTCCTGGAGCAGAAGGTTTGGGAAGTAGCTGCTCCATACATTGAA AAATACAGAATGGAGCATATTCCAGAATCAAGACCTCTTTCTCCAACAGCCTTTTCACTGCAATTTCTG CACAAGAAATCCACCAAAATCCCGGAGTCTGAGGACCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_002108 |
| Insert Size | 1974 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002108.3 |
| RefSeq Size | 3927 bp |
| RefSeq ORF | 1974 bp |
| Locus ID | 3034 |
| UniProt ID | P42357 |
| Protein Families | Druggable Genome |
| Protein Pathways | Histidine metabolism, Metabolic pathways, Nitrogen metabolism |
| MW | 72.7 kDa |
| Gene Summary | Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211651 | HAL (Myc-DDK-tagged)-Human histidine ammonia-lyase (HAL) |
CNY 7,336.00 |
|
| RC211651L1 | Lenti ORF clone of Human histidine ammonia-lyase (HAL), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC211651L2 | Lenti ORF clone of Human histidine ammonia-lyase (HAL), mGFP tagged |
CNY 9,736.00 |
|
| RC211651L3 | Lenti ORF clone of Human histidine ammonia-lyase (HAL), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC211651L4 | Lenti ORF clone of Human histidine ammonia-lyase (HAL), mGFP tagged |
CNY 6,460.00 |
|
| RG211651 | HAL (tGFP-tagged) - Human histidine ammonia-lyase (HAL) |
CNY 8,936.00 |
其它Histidase产品
