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RSPO1 (NM_001038633) Human Untagged Clone
CAT＃: SC302953
RSPO1 (untagged)-Human R-spondin homolog (Xenopus laevis) (RSPO1)
CNY 3,600.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRISTIN3; RSPO |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_001038633 edited
GTTAGCAAGAGCCGTGTGCTATCCACCCTCCCCGAGACCACCCCTCCGACCAGGGGCCTG GAGCTGGCGCGTGACTATGCGGCTTGGGCTGTGTGTGGTGGCCCTGGTTCTGAGCTGGAC GCACCTCACCATCAGCAGCCGGGGGATCAAGGGGAAAAGGCAGAGGCGGATCAGTGCCGA GGGGAGCCAGGCCTGTGCCAAAGGCTGTGAGCTCTGCTCTGAAGTCAACGGCTGCCTCAA GTGCTCACCCAAGCTGTTCATCCTGCTGGAGAGGAACGACATCCGCCAGGTGGGCGTCTG CTTGCCGTCCTGCCCACCTGGATACTTCGACGCCCGCAACCCCGACATGAACAAGTGCAT CAAATGCAAGATCGAGCACTGTGAGGCCTGCTTCAGCCATAACTTCTGCACCAAGTGTAA GGAGGGCTTGTACCTGCACAAGGGCCGCTGCTATCCAGCTTGTCCCGAGGGCTCCTCAGC TGCCAATGGCACCATGGAGTGCAGTAGTCCTGCGCAATGTGAAATGAGCGAGTGGTCTCC GTGGGGGCCCTGCTCCAAGAAGCAGCAGCTCTGTGGTTTCCGGAGGGGCTCCGAGGAGCG GACACGCAGGGTGCTACATGCCCCTGTGGGGGACCATGCTGCCTGCTCTGACACCAAGGA GACCCGGAGGTGCACAGTGAGGAGAGTGCCGTGTCCTGAGGGGCAGAAGAGGAGGAAGGG AGGCCAGGGCCGGCGGGAGAATGCCAACAGGAACCTGGCCAGGAAGGAGAGCAAGGAGGC GGGTGCTGGCTCTCGAAGACGCAAGGGGCAGCAACAGCAGCAGCAGCAAGGGACAGTGGG GCCACTCACATCTGCAGGGCCTGCCTAGGGACACTGTCCAGCCTCCAGGCCCATGCAGAA AGAGTTCAGTGCTACTCTGCGTGATTCAAGCTTTCCTGA >OriGene 5' read for NM_001038633 unedited
NGGGGAAGTTCACATTTGTATACGACTCATATAGGGCGGCCGCGATTCGCCCCANAGTGT TAGCAAGAGCCGTGTGCTATCCACCCTCCCCGANACCACCCCTCCGACCAGGGGCCTGGA GCTGGCGCGTGACTATGCGGCTTGGGCTGTGTGTGGTGGCCCTGGTTCTGAGCTGGACGC ACCTCACCATCAGCAGCCGGGGGATCAAGGGGAAAAGGCAGAGGCGGATCAGTGCCGAGG GGAGCCAGGCCTGTGCCAAAGGCTGTGAGCTCTGCTCTGAAGTCAACGGCTGCCTCAAGT GCTCACCCAAGCTGTTCATCCTGCTGGAGAGGAACGACATCCGCCAGGTGGGCGTCTGCT TGCCGTCCTGCCCACCTGGATACTTCGACGCCCGCAACCCCGACATGAACAAGTGCATCA AATGCAAGATCGAGCACTGTGAGGCCTGCTTCAGCCATAACTTCTGCACCAAGTGTAAGG AGGGCTTGTACCTGCACAAGGGCCGCTGCTATCCAGCTTGTCCCGAGGGCTCCTCAGCTG CCAATGGCACCATGGAGTGCAGTAGTCCTGCGCAATGTGAAATGAGCGAGTGGTCTCCGT GGGGGCCCTGCTCCAAGAAGCAGCAGCTCTGTGGTTTCCGGAGGGGCTCCGAGGAGCGGA CACGCAGGGTGCTACATGCCCCTGTGGGGGACCATGCTGCCTGCTCTGACACCAAGAACC CCGGAGGTGCACAGTGAGGGGAGAGTGCCGTGTCCTGAGGGGGCANAAAGGNAGGAAAAG GAGGGCCAGGGCCCGGNCGGGAGAATGCCAACAGGAACCTGGCCAGNNAAGANAGCAGGA NGGCCGNNTGCTGGCTCTCGAGAACGCAGGGGGCAGCAACAGCAGCAGN >OriGene 3' read for NM_001038633 unedited
NGGGCCCATTGGNGAATGGCACTTCCCAGGNCCAGNANGAGCCTGGGGNAGGGTCACAGG GNAGCCACCCGGGATCTGTTCAGGAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT CAGGAAAGCTTGAATCACGCAGAGTAGCACTGAACTCTTTCTGCATGGGCCTGGAGGCTG GACAGTGTCCCTAGGCAGGCCCTGCAGATGTGAGTGGCCCCACTGTCCCTTGCTGCTGCT GCTGTTGCTGCCCCTTGCGTCTTCGAGAGCCAGCACCCGCCTCCTTGCTCTCCTTCCTGG CCAGGTTCCTGTTGGCATTCTCCCGCCGGCCCTGGCCTCCCTTCCTCCTCTTCTGCCCCT CAGGACACGGCACTCTCCTCACTGTGCACCTCCGGGTCTCCTTGGTGTCAGAGCAGGCAG CATGGTCCCCCACAGGGGCATGTAGCACCCTGCGTGTCCGCTCCTCGGAGCCCCTCCGGA AACCACAGAGCTGCTGCTTCTTGGAGCAGGGCCCCCACGGAGACCACTCGCTCATTTCAC ATTGCGCAGGACTACTGCACTCCATGGTGCCATTGGCAGCTGAGGAGCCCTCGGGACAAG CTGGATAGCAGCGGCCCTTGTGCAGGTACAAGCCCTCCTTACACTTGGTGNCAGAAGTTA TGGCTGAAGCAGGCCTCACAGTGCTCGATCTTGCATTTGATGCACTTGTTCATGTTTCGG GTTTGCGGCGGTTCGAAGTATCCCAGGTGGGCAGGACGGCAAGCAGACGCCCACCTGNNC GGATGTCGTTCCTCNTCCAGCAGATGAACAGCCTNGGGTGAGCACTTGAGGCAGCCGNTG ACTTCAGAGCAGAGCTCACAGNCTTTGGCACAGGCCTGGCTCCCCTCGGNACTGATCCGG CTCTGGCTTTTN |
| Restriction Sites | Please inquire |
| ACCN | NM_001038633 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001038633.2, NP_001033722.1 |
| RefSeq Size | 2995 bp |
| RefSeq ORF | 792 bp |
| Locus ID | 284654 |
| UniProt ID | Q2MKA7 |
| Protein Families | Secreted Protein |
| Gene Summary | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Reconstitution of R-Spondin:LGR4:ZNRF3 Adult Stem Cell Growth Factor Signaling Complexes with Recombinant Proteins Produced in Escherichia coli
,Moad, HE;Pioszak, AA;,
Biochemistry. 2013 Oct 15;52(41):7295-304.
,PubMed ID 24050775
[RSPO1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217921 | RSPO1 (Myc-DDK-tagged)-Human R-spondin homolog (Xenopus laevis) (RSPO1) |
CNY 3,600.00 |
|
| RC217921L1 | Lenti ORF clone of Human R-spondin homolog (Xenopus laevis) (RSPO1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC217921L2 | Lenti ORF clone of Human R-spondin homolog (Xenopus laevis) (RSPO1), mGFP tagged |
CNY 5,890.00 |
|
| RC217921L3 | Lenti ORF clone of Human R-spondin homolog (Xenopus laevis) (RSPO1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC217921L4 | Lenti ORF clone of Human R-spondin homolog (Xenopus laevis) (RSPO1), mGFP tagged |
CNY 6,000.00 |
|
| RG217921 | RSPO1 (tGFP-tagged) - Human R-spondin homolog (Xenopus laevis) (RSPO1) |
CNY 5,200.00 |
