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ZNF207 (NM_001032293) Human Untagged Clone
CAT＃: SC302631
ZNF207 (untagged)-Human zinc finger protein 207 (ZNF207), transcript variant 2
CNY 7,220.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BuGZ; hBuGZ |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302631 representing NM_001032293.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGTCGCAAGAAGAAGAAGCAGCTGAAGCCGTGGTGCTGGTATTGTAATAGAGATTTTGATGATGAG AAGATCCTTATTCAGCACCAAAAAGCAAAGCATTTTAAATGCCATATATGTCACAAGAAATTGTATACA GGACCTGGCTTAGCTATTCATTGCATGCAGGTACATAAAGAAACAATAGATGCCGTACCAAATGCAATA CCTGGAAGAACAGACATAGAGTTGGAAATATATGGTATGGAAGGTATTCCAGAAAAAGACATGGATGAA AGACGACGACTTCTTGAACAGAAAACACAAGAAAGTCAAAAAAAGAAGCAACAAGATGATTCTGATGAA TATGATGATGACGACTCTGCAGCCTCAACTTCATTTCAGCCACAGCCTGTTCAACCTCAGCAAGGTTAT ATTCCTCCAATGGCACAGCCAGGACTGCCACCAGTACCAGGAGCACCAGGAATGCCTCCAGGCATACCT CCATTAATGCCAGGTGTTCCTCCTCTGATGCCAGGAATGCCACCAGTTATGCCAGGCATGCCACCTGGA TTGCATCATCAGAGAAAATACACCCAGTCATTTTGCGGTGAAAACATAATGATGCCAATGGGTGGAATG ATGCCACCTGGACCAGGAATACCACCTCTGATGCCTGGAATGCCACCAGGTATGCCCCCACCTGTTCCA CGTCCTGGAATTCCTCCAATGACTCAAGCACAGGCTGTTTCAGCGCCAGGTATTCTTAATAGACCACCT GCACCAACAGCAACTGTACCTGCCCCACAGCCTCCAGTTACTAAGCCTCTTTTCCCCAGTGCTGGACAG GCTCAGGCAGCTGTCCAAGGACCTGTTGGTACAGATTTCAAACCCTTAAATAGTACCCCTGCAACAACT ACAGAACCCCCAAAGCCTACATTCCCTGCTTATACACAGTCTACAGCTTCAACAACTAGTACAACAAAT AGTACTGCAGCTAAACCAGCGGCTTCAATAACAAGTAAGCCTGCTACACTTACAACAACTAGTGCAACC AGTAAGTTGATCCATCCAGATGAGGATATATCCCTGGAAGAGAGAAGGGCACAGTTACCTAAGTATCAA CGTAATCTTCCTCGGCCAGGACAGGCCCCCATCGGTAATCCACCAGTTGGACCAATTGGAGGTATGATG CCACCACAGCCAGGCATCCCACAGCAACAAGGAATGAGACCCCCAATGCCACCTCATGGTCAGTATGGT GGTCATCATCAAGGCATGCCAGGATACCTTCCTGGTGCTATGCCCCCGTATGGGCAGGGACCGCCAATG GTGCCCCCTTACCAGGGTGGGCCTCCTCGACCTCCGATGGGAATGAGACCTCCTGTAATGTCGCAAGGT GGCCGTTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001032293 |
| Insert Size | 1392 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001032293.2 |
| RefSeq Size | 2240 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 7756 |
| UniProt ID | O43670 |
| Protein Families | Transcription Factors |
| MW | 49.7 kDa |
| Gene Summary | Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 3. It encodes isoform b, which is shorter than isoform c. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201811 | ZNF207 (Myc-DDK-tagged)-Human zinc finger protein 207 (ZNF207), transcript variant 2 |
CNY 3,656.00 |
|
| RC201811L3 | Lenti ORF clone of Human zinc finger protein 207 (ZNF207), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201811L4 | Lenti ORF clone of Human zinc finger protein 207 (ZNF207), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201811 | ZNF207 (tGFP-tagged) - Human zinc finger protein 207 (ZNF207), transcript variant 2 |
CNY 4,370.00 |
