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LIM kinase 2 (LIMK2) (NM_001031801) Human Untagged Clone
CAT＃: SC302595
LIMK2 (untagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302595 representing NM_001031801.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCAGAATGCCAG GATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAG TTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAG TACCACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTG GTGCAGCATGCCACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGA CTCTCCACAGAGTCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACT GAAGGCAGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTG AAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCCTG GAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTAGCCAGACGAGC CAGACACTTCAGCTGTTGATTGAACATGACCCCGTCTCCCAACGCCTGGACCAGCTGCGGCTGGAGGCC CGGCTCGCTCCTCACATGCAGAATGCCGGACACCCCCACGCCCTCAGCACCCTGGACACCAAGGAGAAT CTGGAGGGGACACTGAGGAGACGTTCCCTAAGGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGC TCCCCAAAGGAGCCCCTGCTGTTCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGC TATTCACAGCAGATCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGG CAGGCTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGATGTGAT GAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACCACCCCAATGTGCTC AAGTTCATTGGTGTGCTGTACAAGGATAAGAAGCTGAACCTCCTGACAGAGTACATTGAGGGGGGCACA CTGAAGGACTTTCTGCGCAGTATGGATCCGTTCCCCTGGCAGCAGAAGGTCAGGTTTGCCAAAGGAATC GCCTCCGGAATGGCCTATTTGCACTCTATGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTC ATCAAGTTGGACAAGACTGTGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAA AGGGCCCCCATGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTAC ACGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGAAAGAGCTATGATGAGACGGTG GATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATTGGGCAGGTGTATGCAGATCCTGACTGCCTT CCCCGAACACTGGACTTTGGCCTCAACGTGAAGCTTTTCTGGGAGAAGTTTGTTCCCACAGATTGTCCC CCGGCCTTCTTCCCGCTGGCCGCCATCTGCTGCAGACTGGAGCCTGAGAGCAGAGCCCCCCCCGGGGCC GCAGGAGAGGGCCCGGGCTGCGCGGATGATGAGGGCCCAGTGAGGCGCCAAGGGAAGGTCACCATCAAG TATGACCCCAAGGAGCTACGGAAGCACCTCAACCTAGAGGAGTGGATCCTGGAGCAGCTCACGCGCCTC TACGACTGCCAGGAAGAGGAGATCTCAGAACTAGAGATTGACGTGGATGAGCTCCTGGACATGGAGAGT GACGATGCCTGGGCTTCCAGGGTCAAGGAGCTGCTGGTTGACTGTTACAAACCCACAGAGGCCTTCATC TCTGGCCTGCTGGACAAGATCCGGGCCATGCAGAAGCTGAGCACACCCCAGAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001031801 |
| Insert Size | 2061 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001031801.1 |
| RefSeq Size | 2914 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 3985 |
| UniProt ID | P53671 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| MW | 77.9 kDa |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201929 | LIMK2 (Myc-DDK-tagged)-Human LIM domain kinase 2 (LIMK2), transcript variant 1 |
CNY 5,024.00 |
|
| RC201929L1 | Lenti ORF clone of Human LIM domain kinase 2 (LIMK2), transcript variant 1, Myc-DDK-tagged |
CNY 7,424.00 |
|
| RC201929L2 | Lenti ORF clone of Human LIM domain kinase 2 (LIMK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201929L3 | Lenti ORF clone of Human LIM domain kinase 2 (LIMK2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201929L4 | Lenti ORF clone of Human LIM domain kinase 2 (LIMK2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201929 | LIMK2 (tGFP-tagged) - Human LIM domain kinase 2 (LIMK2), transcript variant 1 |
CNY 6,624.00 |
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