RhoGAP (ARHGAP5) (NM_001030055) Human Untagged Clone

CAT#: SC302503

ARHGAP5 (untagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 1



  "NM_001030055" in other vectors (6)

CNY 10,450.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
ARHGAP5 mouse monoclonal antibody,clone OTI9H2
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GFI2; p190-B; p190BRhoGAP; RhoGAP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC302503 representing NM_001030055.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGACTCTCTGGGACT
GAAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAATAGATTTGTACGCTCAAAAGCA
GATGAATATTATCCAGAGCATACTTCTGTGCTTAGCACCATTGACTTTGGAGGACGAGTAGTAAACAAT
GATCACTTTTTGTACTGGGGTGACATAATACAAAATAGTGAAGATGGAGTAGAATGCAAAATTCATGTC
ATTGAACAAACAGAGTTCATTGATGACCAGACTTTCTTGCCTCATCGGAGTACGAATTTGCAACCATAT
ATAAAACGTGCAGCTGCATCTAAATTGCAGTCAGCAGAAAAACTAATGTACATTTGCACTGATCAGCTA
GGCTTAGAACAAGACTTTGAACAGAAGCAAATGCCTGAAGGGAAGCTCAACGTAGATGGATTTTTATTA
TGCATTGATGTAAGTCAAGGATGCAATAGGAAGTTTGATGATCAACTTAAATTTGTGAATAACCTTTTT
GTCCAGTTATCAAAATCAAAAAAACCTGTAATAATAGCAGCAACTAAATGTGATGAATGCGTGGATCAT
TATCTTAGAGAAGTTCAGGCATTTGCTTCAAATAAAAAGAACCTTCTTGTAGTGGAAACATCAGCACGA
TTTAATGTCAACATTGAAACATGTTTTACTGCACTGGTACAAATGTTGGATAAAACTCGTAGCAAGCCT
AAAATTATTCCCTATTTGGATGCTTATAAAACACAGAGACAACTTGTTGTCACAGCAACAGATAAGTTT
GAAAAACTTGTGCAGACTGTGAGAGATTATCATGCAACTTGGAAAACTGTTAGTAATAAATTAAAAAAT
CATCCTGATTATGAAGAATACATCAACTTAGAGGGAACAAGAAAGGCCAGAAATACATTCTCAAAACAT
ATAGAACAACTTAAACAGGAACATATAAGAAAAAGGAGAGAAGAGTATATAAATACTTTACCAAGAGCT
TTTAACACTCTTTTGCCAAATCTAGAAGAGATTGAACATTTGAATTGGTCAGAAGCTTTGAAGTTAATG
GAAAAGAGAGCAGATTTCCAGTTATGTTTTGTGGTGCTAGAAAAAACTCCTTGGGATGAAACTGACCAT
ATAGACAAAATTAATGATAGGCGGATTCCATTTGACCTCCTGAGCACTTTAGAAGCTGAAAAAGTCTAT
CAGAACCATGTACAGCATCTGATATCCGAGAAGAGGAGGGTGGAAATGAAGGAAAAATTCAAAAAGACT
TTGGAAAAAATTCAATTCATTTCACCAGGGCAGCCATGGGAGGAAGTTATGTGCTTTGTTATGGAGGAT
GAAGCCTACAAATATATCACTGAGGCTGATAGCAAAGAGGTATATGGTAGGCATCAGCGAGAAATAGTT
GAAAAAGCCAAAGAAGAGTTTCAAGAAATGCTTTTTGAGCATTCTGAACTTTTTTATGATTTAGATCTT
AATGCAACACCTAGTTCAGATAAAATGAGTGAAATTCATACAGTTCTGAGTGAAGAACCTAGATATAAA
GCTTTACAGAAACTTGCACCTGATAGGGAATCCCTTCTACTTAAGCATATAGGATTTGTTTATCATCCC
ACTAAAGAAACATGTCTTAGTGGCCAAAATTGTACAGACATTAAAGTGGAGCAGTTACTTGCTAGTAGT
CTTTTACAGTTGGATCATGGCCGCTTAAGATTATATCACGATAGTACCAATATAGATAAAGTTAACCTT
TTTATTTTAGGGAAGGATGGCCTTGCCCAAGAACTAGCAAATGAGATAAGGACACAATCCACTGATGAT
GAGTATGCCTTAGATGGAAAAATTTATGAACTTGATCTTCGGCCGGTTGATGCCAAATCGCCTTACTTT
TTGAGTCAGTTATGGACTGCCGCCTTTAAACCACATGGGTGCTTCTGTGTATTTAATTCCATTGAGTCA
TTGAGTTTTATTGGGGAATTTATTGGGAAAATAAGAACTGAAGCTTCTCAGATCAGAAAAGATAAATAC
ATGGCTAATCTTCCATTTACATTAATTCTGGCTAATCAGAGAGATTCCATTAGTAAGAATCTACCAATT
CTCAGGCACCAAGGGCAGCAGTTGGCAAACAAGTTGCAATGTCCTTTTGTAGATGTACCTGCTGGTACA
TATCCTCGTAAATTTAATGAAACCCAAATAAAGCAAGCTCTCAGAGGAGTATTGGAATCAGTTAAACAC
AATTTGGATGTGGTGAGCCCAATTCCTGCCAATAAGGACTTATCAGAAGCTGACTTGAGAATTGTCATG
TGCGCCATGTGTGGAGATCCATTTAGTGTGGATCTTATTCTTTCACCCTTCCTTGATTCTCATTCTTGC
AGTGCTGCTCAAGCTGGACAGAATAATTCCCTAATGCTTGATAAAATCATTGGTGAAAAAAGGAGGCGA
ATACAGATCACAATATTATCATACCACTCTTCAATTGGAGTAAGAAAAGATGAACTAGTTCATGGGTAT
ATATTAGTTTACTCTGCAAAACGGAAAGCTTCGATGGGAATGCTTCGAGCATTTCTATCAGAAGTTCAA
GACACCATTCCTGTACAGCTGGTGGCAGTTACTGACAGCCAAGCAGATTTTTTTGAAAATGAGGCTATC
AAAGAGTTAATGACTGAAGGAGAACACATTGCAACTGAGATCACTGCTAAATTTACAGCACTGTATTCT
TTATCTCAGTATCATCGGCAAACTGAGGTCTTTACTCTGTTTTTTAGTGATGTTCTAGAGAAAAAAAAT
ATGATAGAAAATTCTTATTTGTCTGATAATACAAGGGAATCAACCCATCAAAGTGAAGATGTTTTTCTA
CCATCTCCCAGAGACTGTTTTCCCTATAATAACTACCCTGATTCAGATGATGACACAGAAGCACCACCT
CCTTATAGTCCAATTGGGGATGATGTACAGTTGCTTCCAACACCTAGTGACCGTTCCAGATATAGATTA
GATTTGGAAGGAAATGAGTATCCTATTCATAGTACCCCAAACTGTCATGACCATGAACGCAACCATAAA
GTGCCTCCACCTATTAAACCTAAACCAGTTGTACCTAAGACAAATGTGAAAAAACTCGATCCAAACCTT
TTAAAAACAATTGAAGCTGGTATTGGTAAAAATCCAAGAAAGCAGACTTCCCGGGTGCCTTTGGCACAT
CCTGAAGATATGGATCCTTCAGATAACTATGCGGAACCCATTGATACAATTTTCAAACAGAAGGGCTAT
TCTGATGAGATTTATGTTGTCCCAGATGATAGTCAAAATCGTATTAAAATTCGAAACTCATTTGTAAAT
AACACCCAAGGAGATGAAGAAAATGGGTTTTCTGATAGAACCTCAAAAAGTCATGGGGAACGGAGGCCT
TCAAAATACAAATATAAATCTAAAACCTTGTTTAGTAAAGCCAAGTCATACTATAGAAGAACACATTCA
GATGCCAGTGATGATGAGGCTTTCACCACTTCTAAAACAAAAAGAAAAGGAAGACATCGTGGAAGTGAA
GAAGATCCACTTCTTTCTCCTGTTGAAACTTGGAAAGGTGGTATTGATAATCCTGCAATCACTTCTGAC
CAGGAGTTAGATGATAAGAAGATGAAGAAGAAAACCCACAAAGTGAAAGAAGATAAAAAGCAGAAAAAG
AAAACTAAGAACTTCAATCCACCAACACGTAGAAATTGGGAAAGTAATTACTTTGGGATGCCCCTCCAG
GATCTGGTTACAGCTGAGAAGCCCATACCACTATTTGTTGAGAAATGTGTGGAATTTATTGAAGATACA
GGGTTATGTACCGAAGGACTCTACCGTGTCAGCGGGAATAAAACTGACCAAGACAATATTCAAAAGCAG
TTTGATCAAGATCATAATATCAATCTAGTGTCAATGGAAGTAACAGTAAATGCTGTAGCTGGAGCCCTT
AAAGCTTTCTTTGCAGATCTGCCAGATCCTTTAATTCCATATTCTCTTCATCCAGAACTATTGGAAGCA
GCAAAAATCCCGGATAAAACAGAACGTCTTCATGCCTTGAAAGAAATTGTTAAGAAATTTCATCCTGTA
AACTATGATGTATTCAGATACGTGATAACACATCTAAACAGGGTTAGTCAGCAACATAAAATCAACCTA
ATGACAGCAGACAACTTATCCATCTGTTTTTGGCCAACCTTGATGAGACCTGATTTTGAAAATCGAGAG
TTTCTGTCTACTACTAAGATTCATCAATCTGTTGTTGAAACATTCATTCAGCAGTGTCAGTTTTTCTTT
TACAATGGAGAAATTGTAGAAACGACAAACATTGTGGCTCCTCCACCACCTTCAAACCCAGGACAGTTG
GTGGAACCAATGGTGCCACTTCAGTTGCCGCCACCATTGCAACCTCAGCTGATACAACCACAATTACAA
ACGGATCCTCTTGGTATTATATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001030055
Insert Size 4509 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001030055.1
RefSeq Size 9604 bp
RefSeq ORF 4509 bp
Locus ID 394
UniProt ID Q13017
Protein Pathways Focal adhesion, Leukocyte transendothelial migration
MW 172.5 kDa
Gene Summary Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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