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VRK3 (NM_001025778) Human Untagged Clone
CAT＃: SC302430
VRK3 (untagged)-Human vaccinia related kinase 3 (VRK3), transcript variant 2
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302430 representing NM_001025778.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCTCCTTCTGTCCAGACTGTGGCAAAAGTATCCAAGCGGCATTCAAATTCTGCCCCTACTGTGGA AATTCTTTGCCTGTAGAGGAGCATGTAGGGTCCCAGACCTTTGTCAATCCACATGTGTCATCCTTCCAA GGCTCCGGGAGCAGACCCCCAACCCCCAAAAGCAGCCCTCAGAAGACCAGGAAGAGCCCTCAGGTGACC AGGGGTAGCCCTCAGAAGACCAGCTGTAGCCCTCAGAAGACCAGGCAGAGCCCTCAGACGCTGAAGCGG AGCCGAGTGACCACCTCACTTGAAGCTTTGCCCACAGGGACAGTGCTGACAGACAAGAGTGGGCGACAG TGGAAGCTGAAGTCCTTCCAGACCAGGGACAACCAGGGCATTCTCTATGAAGCTGCACCCACCTCCACC CTCACCTGTGACTCAGGACCACAGAAGCAAAAGTTCTCACTCAAACTGGATGCCAAGGATGGGCGCTTG TTCAATGAGCAGAACTTCTTCCAGCGGGCCGCCAAGCCTCTGCAAGTCAACAAGTGGAAGAAGCTGTAC TCGACCCCACTGCTGGCCATCCCTACCTGCATGGGTTTCGGTGTTCACCAGGACAAATACAGGTTCTTG GTGTTACCCAGCCTGGGGAGGAGCCTTCAGTCGGCCCTGGATGTCAGCCCAAAGCATGTGCTGTCAGAG AGGTCTGTGCTGCAGGTGGCCTGCCGGCTGCTGGATGCCCTGGAGTTCCTCCATGAGAATGAGTATGTT CATGGAAATGTGACAGCTGAAAATATCTTTGTGGATCCAGAGGACCAGAGTCAGGTGACTTTGGCAGGC TATGGCTTCGCCTTCCGCTATTGCCCAAGTGGCAAACACGTGGCCTACGTGGAAGGCAGCAGGAGCCCT CACGAGGGGGACCTTGAGTTCATTAGCATGGACCTGCACAAGGGATGCGGGCCCTCCCGCCGCAGCGAC CTCCAGAGCCTGGGCTACTGCATGCTGAAGTGGCTCTACGGGTTTCTGCCATGGACAAATTGCCTTCCC AACACTGAGGACATCATGAAGCAAAAACAGAAGTTTGTTGATAAGCCGGGGCCCTTCGTGGGACCCTGC GGTCACTGGATCAGGCCCTCAGAGACCCTGCAGAAGTACCTGAAGGTGGTGATGGCCCTCACGTATGAG GAGAAGCCGCCCTACGCCATGCTGAGGAACAACCTAGAAGCTTTGCTGCAGGATCTGCGTGTGTCTCCA TATGACCCCATTGGCCTCCCGATGGTGCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001025778 |
| Insert Size | 1275 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025778.1 |
| RefSeq Size | 1979 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 51231 |
| UniProt ID | Q8IV63 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 47.5 kDa |
| Gene Summary | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a protein (isoform 2) that is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216625 | VRK3 (Myc-DDK-tagged)-Human vaccinia related kinase 3 (VRK3), transcript variant 2 |
CNY 3,656.00 |
|
| RC216625L3 | Lenti ORF clone of Human vaccinia related kinase 3 (VRK3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216625L4 | Lenti ORF clone of Human vaccinia related kinase 3 (VRK3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG216625 | VRK3 (tGFP-tagged) - Human vaccinia related kinase 3 (VRK3), transcript variant 2 |
CNY 4,370.00 |
