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RFX5 (NM_001025603) Human Untagged Clone
CAT＃: SC302426
RFX5 (untagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302426 representing NM_001025603.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGAAGATGAGCCTGATGCTAAGAGCCCCAAGACTGGGGGAAGGGCCCCCCCAGGTGGTGCTGAG GCTGGGGAACCTACCACCCTTCTTCAGAGGCTCCGAGGTACCATTTCCAAGGCCGTGCAGAACAAAGTA GAGGGGATCCTGCAAGATGTACAGAAATTTTCTGACAATGACAAGCTGTATCTCTACCTTCAGCTCCCC TCAGGACCCACCACTGGAGACAAAAGCTCAGAGCCAAGTACACTGAGCAATGAGGAGTACATGTATGCC TATAGGTGGATCCGCAACCACCTGGAAGAGCACACTGACACCTGTCTGCCAAAGCAAAGTGTTTATGAT GCCTATCGGAAGTACTGTGAGAGTCTTGCCTGTTGCCGCCCACTCAGCACAGCCAACTTTGGCAAGATC ATCAGAGAGATCTTCCCTGACATCAAAGCTCGAAGGCTTGGTGGCCGGGGCCAGTCCAAATATTGCTAC AGTGGCATAAGGAGGAAGACCTTGGTGTCTATGCCACCCCTGCCTGGACTTGACCTAAAGGGTTCTGAG AGTCCAGAAATGGGCCCAGAAGTAACCCCAGCACCTCGAGATGAACTGGTGGAGGCAGCGTGTGCCCTG ACCTGTGACTGGGCAGAGCGGATCCTGAAACGGTCCTTCAGTTCCATCGTTGAGGTCGCCCGCTTCCTG CTACAGCAGCATCTCATCTCTGCCCGATCTGCACATGCCCATGTGCTTAAGGCCATGGGGCTTGCTGAA GAGGACGAACATGCACCTCGGGAACGGTCATCTAAACCAAAGAATGGTTTAGAGAACCCAGAGGGTGGA GCCCACAAGAAGCCAGAGAGACTGGCCCAGCCTCCTAAGGATCTGGAAGCCCGAACTGGGGCCGGTCCT CTCGCACGTGGAGAGCGGAAGAAGAGTGTAGTTGAGAGCTCGGCCCCAGGAGCCAATAACCTGCAGGTT AATGCCCTAGTGGCTCGGCTGCCTCTGCTCCTTCCCCGGGCCCCTCGCTCACTAATTCCGCCAATCCCA GTCTCTCCACCTATTCTGGCCCCCAGGCTTTCTTCAGGTGCCCTGAAAGTGGCTACACTGCCTCTGTCT AGTAGGGCCGGGGCACCCCCAGCAGCTGTGCCCATCATTAACATGATCTTACCAACTGTTCCTGCTTTG CCTGGACCTGGACCTGGGCCTGGGCGAGCTCCACCTGGGGGACTCACTCAGCCCCGGGGCACAGAGAAC AGAGAGGTAGGCATAGGTGGTGACCAAGGACCACATGACAAGGGTGTCAAGAGGACAGCTGAAGTACCT GTGAGTGAGGCCAGTGGGCAGGCTCCACCAGCTAAAGCAGCAAAGCAGGATATAGAGGATACAGCAAGT GATGCCAAAAGGAAACGGGGGCGCCCTCGAAAAAAGTCAGGTGGAAGTGGGGAAAGGAATTCTACCCCT CTCAAGTCAGCAGCTGCCATGGAATCTGCCCAGTCCTCAAGGTTACCATGGGAGACATGGGGCTCAGGA GGGGAAGGCAACTCAGCTGGAGGGGCAGAGAGGCCAGGGCCAATGGGAGAGGCTGAAAAGGGGGCAGTA CTTGCCCAGGGTCAGGGAGATGGTACTGTTTCCAAAGGAGGAAGGGGCCCCGGTTCCCAGCATACCAAA GAAGCAGAAGATAAAATTCCCTTGGTCCCCTCAAAAGTGAGTGTCATCAAGGGCAGCAGAAGCCAAAAG GAGGCTTTTCCTTTGGCAAAGGGAGAGGTAGACACTGCACCACAGGGTAATAAAGACTTAAAGGAGCAT GTGCTTCAAAGTTCCTTATCCCAGGAGCATAAAGACCCAAAAGCAACACCCCCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001025603 |
| Insert Size | 1851 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025603.1 |
| RefSeq Size | 3611 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 5993 |
| UniProt ID | P48382 |
| Protein Families | Transcription Factors |
| Protein Pathways | Antigen processing and presentation, Primary immunodeficiency |
| MW | 65.3 kDa |
| Gene Summary | A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212448 | RFX5 (Myc-DDK-tagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2 |
CNY 4,584.00 |
|
| RC212448L3 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212448L4 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212448 | RFX5 (tGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 2 |
CNY 4,370.00 |
