AP2M1 (NM_001025205) Human Untagged Clone
CAT#: SC302369
AP2M1 (untagged)-Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AP50; CLAPM1; MRD60; mu2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC302369 representing NM_001025205.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCTACCGAGATGAC ATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCCGGCAGCAGGTGCGCAGCCCC GTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGCGGTCCAACATTTGGCTGGCAGCAGTCACC AAGCAGAATGTCAACGCTGCCATGGTCTTCGAATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTAC TTTGGCAAGATCAGCGAGGAAAACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATT CTAGACTTTGGCTACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATC AAGAGTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGCGGCGA GAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGAACCTGCTCATGTCC CCACAAGGGCAGGTGCTGAGTGCCCATGTGTCGGGCCGGGTGGTGATGAAGAGCTACCTGAGTGGCATG CCTGAATGCAAGTTTGGGATGAATGACAAGATTGTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAA ACAAGCAAGAGCGGGAAGCAATCAATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGC AAGTTTGACTCTGAACGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTATCGC ACAACCAAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGACGCACCAAACTG GAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCTCAGAAGATCGAGGTGAGGATC CCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGCATGAAGGGGAAGGCCAAGTACAAGGCCAGC GAGAATGCCATCGTGTGGAAGATCAAGCGCATGGCAGGCATGAAGGAATCGCAGATCAGCGCAGAGATT GAGCTTCTGCCTACCAACGACAAGAAGAAATGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCA TTCGCGCCCTCTGGCCTCAAGGTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCAT GATGTCATCAAATGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001025205 |
Insert Size | 1302 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001025205.1 |
RefSeq Size | 1952 bp |
RefSeq ORF | 1302 bp |
Locus ID | 1173 |
UniProt ID | Q96CW1 |
Protein Families | Druggable Genome |
Protein Pathways | Endocytosis, Huntington's disease |
MW | 49.4 kDa |
Gene Summary | This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (2) lacks three alternate in-frame exons compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform c. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201377 | AP2M1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2 |
CNY 3,656.00 |
|
RC201377L1 | Lenti ORF clone of Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC201377L2 | Lenti ORF clone of Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC201377L3 | Lenti ORF clone of Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201377L4 | Lenti ORF clone of Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG201377 | AP2M1 (tGFP-tagged) - Human adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 2 |
CNY 5,256.00 |