DHODH (NM_001025193) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DHOdehase; dihydroorotate dehydrogenase; dihydroorotate oxidase; human complement of yeast URA1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_001025193 edited ATGGCGTGGAGACACCTGCAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGA GGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACAC CTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGC TTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTG AGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATTGCTGCAGGATTTGACAAGCAT GGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTG ACTCCAAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAA GCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTA CGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGTTGGGCATCGATGGGCTGATTGT TACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACAGG AGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTATGC ACTCACCCAAGGCCGAGTTCCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGACGT GCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTTCTG GGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAGGG CTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCATCGGAGGTGAGGACAGCGTCTGAC GGGAAGCCTGATCTGGAACCTTCCCAAGGACTCAGGCAAGCCTTTGTGGCTGGATCATGA GAGGAGGGACTCCATCTTGAGCCATGTCCCCCAGCCATGGCATGGCTGCACTGTAAACGC CAATCGGGGGGTCACCAGGATCAACCGCAGGCTTTCTTCAGTCCCTTGGTCAGACCATAA ACTGCATTTTTGATTCTTTGTGGATTCAAACCCTAGGATCCATCAGTCTTGCAAGGACAT TGAATATTAGGAGGAAAAAGTCATGGAAAAAATAAAGCCATTTAGAACCTGGGAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_001025193 unedited GGACGTCATATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAAAGGCGTGG AGACACCTGCAAAACGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCT TCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGA CTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGCTTCACCTCCC TGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGG GCCATAAATTCCGAAATCCAGTAGGAATTGGCTGCAGGATTTGACAAGCATGGGGAAGCC GTGGACGGACTTTATAAGATGGGCTTNNTTGGGTTTTGTTGAGATAGGAAGTGTGACTCC AAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAAGCTGT CATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTACGGGC CAGACAGCAGAAGCAGGCCAAGCTCACAGAAGTTGGGCATCGATGGGCTGATTGTTACGA ACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACAGGAGGGC TGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTATGCACTCA CCCAAGGCCGAGTTCCCATAATTGGGGTTGGGTGGTGTGAGCAGCGGGCANGACGTGCTG GAGAAGATCCGGGCAGGGGCCTNCCTGGTGCAGCTGTACACGGCCCTCACCTTCTGGGGG CCACCGTTGTGGGCAAAGTCAAGCGGGC
Restriction Sites	Please inquire
ACCN	NM_001025193
Insert Size	1200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acids.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001025193.1, NP_001020364.1
RefSeq Size	1778 bp
RefSeq ORF	594 bp
Locus ID	1723
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Metabolic pathways, Pyrimidine metabolism
Gene Summary	The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]

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