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KRIT1 (NM_001013406) Human Untagged Clone
CAT＃: SC301780
KRIT1 (untagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 5
CNY 13,300.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAM; CCM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301780 representing NM_001013406.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAAATCCAGAAAACATAGAAGATGCATATGTTGCTGTTATTCGTCCAAAGAATACTGCCAGTCTC AATTCTCGGGAATACAGAGCTAAGTCATATGAAATTTTGTTGCATGAAGTTCCCATTGAAGGACAGAAA AAAAAGAGAAAGAAAGTTTTATTGGAAACGAAACTTCAAGGCAACAGTGAAATAACACAAGGCATATTG GATTACGTAGTAGAAACCACCAAACCAATTTCTCCTGCAAACCAGGGTATCAGAGGAAAACGAGTTGTA CTAATGAAAAAATTTCCTCTGGATGGAGAGAAGATGGGCAGAGAAGCATCATTATTTATTGTTCCATCA GTTGTCAAAGATAATACTAAATACACATATACCCCAGGATGCCCAATTTTTTACTGCTTACAAGATATT ATGCGAGTCTGTAGTGAATCCAGTACTCATTTTGCTACACTTACAGCAAGGATGTTAATAGCCTTGGAT AAGTGGTTAGATGAACGTCATGCACAATCTCACTTTATTCCAGCTTTATTCCGACCTTCTCCTCTTGAG CGGATAAAAACTAATGTCATAAATCCTGCATATGCTACTGAATCAGGTCAGACAGAAAACTCACTACAT ATGGGCTATAGTGCACTAGAAATAAAGAGTAAAATGTTAGCCCTAGAGAAAGCAGATACCTGTATTTAC AACCCTTTGTTTGGATCAGATCTTCAGTATACAAATCGGGTAGATAAAGTGGTAATAAATCCATACTTT GGTCTAGGAGCTCCAGACTACTCAAAAATCCAAATACCTAAACAGGAAAAATGGCAGAGAAGCATGAGC AGTGTCACAGAAGACAAGTATGGAAAAGTTGAGGCCACTCGCATATTGTTAGAGAAAGGAAAGTGCAAT CCAAACCTTTTAAATGGACAACTTAGTTCTCCTCTTCATTTTGCTGCTGGAGGAGGACATGCTGAAATA GTACAGATTCTCCTAAACCACCCAGAAACGGATAGACATATAACAGACCAACAAGGAAGATCTCCATTA AATATTTGTGAAGAAAACAAACAAAACAACTGGGAAGAAGCTGCAAAATTGTTGAAGGAAGCAATTAAC AAACCATATGAAAAAGTTCGAATATACAGAATGGATGGGTCATATCGTTCTGTTGAATTGAAGCATGGA AATAATACCACAGTGCAGCAGATAATGGAAGGAATGCGTCTCTCTCAAGAAACTCAGCAATATTTCACT ATATGGATTTGTTCAGAAAACCTCAGCCTTCAACTCAAACCATATCATAAACCCTTGCAACATGTTCGT GACTGGCCAGAAATACTTGCTGAATTGACTAATCTGGATCCTCAAAGGGAAACACCTCAGCTTTTTCTA AGAAGAGATGTGAGACTTCCCTTGGAAGTTGAAAAACAGATTGAAGACCCACTAGCTATTCTTATTCTC TTTGATGAAGCCAGATATAATTTATTGAAGGGCTTTTATACAGCTCCTGATGCTAAGCTGATAACATTG GCAAGTCTGCTTTTGCAAATAGTCTATGGAAATTATGAGAGTAAAAAACACAAGCAAGGTTTCCTAAAT GAAGAAAATCTAAAATCCATCGTACCTGTTACCAAACTGAAAAGTAAGGCACCTCACTGGACAAATCGC ATACTTCATGAATACAAGAATCTCAGTACAAGTGAAGGTGTCAGTAAAGAAATGCATCACCTTCAGCGC ATGTTCTTACAGAATTGCTGGGAAATTCCTACTTATGGAGCAGCATTTTTCACAGGACAGATATTTACA AAGGCAAGCCCCAGCAATCATAAAGTCATCCCTGTGTATGTAGGAGTGAATATAAAAGGACTTCATCTC CTCAACATGGAAACTAAGGCTTTACTCATCAGTCTTAAGTATGGTTGTTTTATGTGGCAATTGGGAGAT ACTGATACTTGTTTTCAGATCCATAGCATGGAAAATAAAATGAGCTTTATAGTACATACAAAACAGGCT GGTCTCGTGGTAAAACTGTTAATGAAGCTAAATGGACAGTTAATGCCCACTGAAAGAAATTCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001013406 |
| Insert Size | 2067 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013406.1 |
| RefSeq Size | 4231 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 889 |
| UniProt ID | O00522 |
| Protein Families | Druggable Genome |
| MW | 78.6 kDa |
| Gene Summary | This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (5) has an alternate 5' exon, and lacks 2 exons in the 5' UTR and an in-frame exon in the coding region, as compared to variant 1. The encoded isoform 2 lacks an internal segment, as compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215470 | KRIT1 (Myc-DDK-tagged)-Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 5 |
CNY 5,040.00 |
|
| RC215470L3 | Lenti ORF clone of Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 5, Myc-DDK-tagged |
CNY 9,120.00 |
|
| RC215470L4 | Lenti ORF clone of Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 5, mGFP tagged |
CNY 9,120.00 |
|
| RG215470 | KRIT1 (tGFP-tagged) - Human KRIT1, ankyrin repeat containing (KRIT1), transcript variant 5 |
CNY 7,980.00 |
