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Salivary alpha amylase (AMY1A) (NM_001008221) Human Untagged Clone
CAT＃: SC301262
AMY1A (untagged)-Human amylase, alpha 1A (salivary) (AMY1A), transcript variant 2
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMY1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301262 representing NM_001008221.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGCTCTTTTGGTTGCTTTTCACCATTGGGTTCTGCTGGGCTCAGTATTCCTCAAATACACAACAA GGACGAACATCTATTGTTCATCTGTTTGAATGGCGATGGGTTGATATTGCTCTTGAATGTGAGCGATAT TTAGCTCCCAAGGGATTTGGAGGGGTTCAGGTCTCTCCACCAAATGAAAATGTTGCCATTCACAACCCT TTCAGACCTTGGTGGGAAAGATACCAACCAGTTAGCTATAAATTATGCACAAGATCTGGAAATGAAGAT GAATTTAGAAACATGGTGACTAGATGCAACAATGTTGGGGTTCGTATTTATGTGGATGCTGTAATTAAT CATATGTGTGGTAATGCTGTGAGTGCAGGAACAAGCAGTACCTGTGGAAGTTACTTCAACCCTGGAAGT AGGGACTTTCCAGCAGTCCCATATTCTGGATGGGATTTTAATGATGGTAAATGTAAAACTGGAAGTGGA GATATCGAGAACTATAATGATGCTACTCAGGTCAGAGATTGTCGTCTGTCTGGTCTTCTCGATCTTGCA CTGGGGAAGGATTATGTGCGTTCTAAGATTGCCGAATATATGAACCATCTCATTGACATTGGTGTTGCA GGGTTCAGAATTGATGCTTCCAAGCACATGTGGCCTGGAGACATAAAGGCAATTTTGGACAAACTGCAT AATCTAAACAGTAACTGGTTCCCGGAAGGTAGTAAACCTTTCATTTACCAGGAGGTAATTGATCTGGGT GGTGAGCCAATTAAAAGCAGTGACTACTTTGGTAATGGCCGGGTGACAGAATTCAAGTATGGTGCAAAA CTCGGCACAGTTATTCGCAAGTGGAATGGAGAGAAGATGTCTTACTTAAAGAACTGGGGAGAAGGTTGG GGTTTCATGCCTTCTGACAGAGCGCTTGTCTTTGTGGATAACCATGACAATCAACGAGGACATGGCGCT GGAGGAGCCTCTATACTTACCTTCTGGGATGCTAGGCTGTACAAAATGGCAGTTGGATTTATGCTTGCT CATCCTTATGGATTTACACGAGTAATGTCAAGCTACCGTTGGCCAAGATATTTTGAAAATGGAAAAGAT GTTAATGATTGGGTTGGGCCACCAAATGATAATGGAGTAACTAAAGAAGTTACTATTAATCCAGACACT ACTTGTGGCAATGACTGGGTCTGTGAACATCGATGGCGCCAAATAAGGAACATGGTTAATTTCCGCAAT GTAGTGGATGGCCAGCCTTTTACAAACTGGTATGATAATGGGAGCAACCAAGTGGCTTTTGGGAGAGGA AACAGAGGATTCATTGTTTTCAACAATGATGACTGGACATTTTCTTTAACTTTGCAAACTGGTCTTCCT GCTGGCACATACTGTGATGTCATTTCTGGAGATAAAATTAATGGCAACTGCACAGGCATTAAAATCTAC GTTTCTGATGATGGCAAAGCTCATTTTTCTATTAGTAACTCTGCTGAAGATCCATTTATTGCAATTCAT GCTGAATCTAAATTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001008221 |
| Insert Size | 1536 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008221.1 |
| RefSeq Size | 1781 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 276 |
| UniProt ID | P04745 |
| Protein Families | ES Cell Differentiation/IPS, Secreted Protein |
| Protein Pathways | Metabolic pathways, Starch and sucrose metabolism |
| MW | 57.8 kDa |
| Gene Summary | Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209386 | AMY1A (Myc-DDK-tagged)-Human amylase, alpha 1A (salivary) (AMY1A), transcript variant 2 |
CNY 3,808.00 |
|
| RC209386L3 | Lenti ORF clone of Human amylase, alpha 1A (salivary) (AMY1A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209386L4 | Lenti ORF clone of Human amylase, alpha 1A (salivary) (AMY1A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG209386 | AMY1A (tGFP-tagged) - Human amylase, alpha 1A (salivary) (AMY1A), transcript variant 2 |
CNY 4,370.00 |
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