F8A2 (NM_001007523) Human Untagged Clone

CAT#: SC301219

F8A2 (untagged)-Human coagulation factor VIII-associated 2 (F8A2)



  "NM_001007523" in other vectors (4)

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HAP40
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC301219 representing NM_001007523.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGGCAGCGGCTGCAGGCCTGGGCGGCGGCGGCGCCGGCCCGGGACCCGAGGCCGGGGACTTCCTG
GCCCGCTACCGGCTGGTATCGAACAAGCTGAAGAAGCGGTTCCTGCGGAAGCCGAACGTGGCGGAGGCC
GGCGAGCAGTTCGGACAGCTGGGCCGGGAGCTGCGCGCCCAGGAGTGTCTGCCCTACGCGGCCTGGTGC
CAGCTGGCGGTGGCGCGCTGCCAGCAGGCGCTCTTCCACGGGCCCGGGGAGGCGCTGGCCCTCACCGAG
GCCGCCCGCCTCTTCCTGCGGCAGGAGCGCGACGCGCGCCAGCGCCTGGTCTGCCCCGCCGCCTACGGG
GAGCCGCTGCAGGCCGCCGCCAGCGCCCTGGGCGCCGCGGTGCGTCTGCACCTCGAGCTGGGCCAGCCG
GCCGCCGCCGCCGCCCTCTGCCTCGAGCTGGCCGCCGCCCTGCGCGACCTGGGCCAGCCGGCCGCCGCC
GCCGGTCACTTCCAGCGCGCCGCCCAGCTCCAGCTGCCCCAGCTGCCCCTGGCCGCGCTGCAGGCGCTT
GGCGAGGCCGCCTCCTGCCAGCTGCTGGCGCGCGACTACACCGGCGCCCTGGCGGTCTTCACGCGCATG
CAGCGCCTGGCGCGGGAGCACGGCAGCCACCCGGTGCAGTCACTGCCGCCGCCCCCGCCGCCGGCACCC
CAGCCCGGGCCCGGGGCGACGCCCGCCCTACCGGCCGCGCTGCTTCCTCCGAACTCCGGCTCGGCGGCG
CCCTCTCCCGCCGCCCTGGGCGCCTTCTCGGACGTGCTGGTCCGCTGCGAGGTGTCCCGCGTGCTGCTG
CTGCTCCTCCTGCAACCACCGCCCGCCAAGCTGCTGCCGGAGCACGCCCAGACCCTGGAGAAGTACTCC
TGGGAGGCTTTTGACAGCCACGGGCAGGAGAGCAGCGGCCAGCTTCCCGAGGAGCTCTTTCTGCTGCTC
CAGTCTTTGGTCATGGCTACCCACGAAAAGGACACGGAAGCCATCAAGTCGCTGCAGGTGGAGATGTGG
CCACTGTTGACTGCTGAGCAGAACCACCTCCTTCACCTCGTTCTGCAAGAAACCATCTCCCCCTCAGGA
CAGGGAGTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001007523
Insert Size 1116 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001007523.1
RefSeq Size 1116 bp
RefSeq ORF 1116 bp
Locus ID 474383
UniProt ID P23610
MW 39.1 kDa
Gene Summary This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the middle copy. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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