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DEPDC5 (NM_001007188) Human Untagged Clone
CAT＃: SC301164
DEPDC5 (untagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 2
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEP.5; FFEVF; FFEVF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC301164 representing NM_001007188.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAACAACAAAGGTCTACAAACTCGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTA GTTGTGAACCCCAAAGTGTTCCCTCACATCAAGCTTGGAGACATTGTAGAGATTGCACACCCCAACGAT GAATACAGCCCTCTGCTTTTGCAGGTCAAGTCTCTTAAGGAAGATTTACAGAAGGAAACTATCAGTGTG GACCAGACTGTGACTCAAGTGTTCCGGCTGAGACCTTATCAGGATGTCTATGTTAATGTCGTAGACCCT AAGGATGTGACCCTTGACCTAGTGGAATTAACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGG CGACTAAAGAAAAGTTTGGTCAGCACATGTGCCTATATCACCCAGAAGGTGGAGTTTGCTGGCATCAGA GCACAGGCTGGTGAACTGTGGGTTAAGAATGAGAAGGTCATGTGTGGCTACATCAGTGAAGATACCAGG GTGGTGTTTCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTT GATATTTATGGGGATTTGTATTTTGAGAAAGCTGTGAATGGTTTCCTTGCTGATCTATTTACCAAGTGG AAGGAGAAGAACTGTAGTCATGAAGTGACAGTGGTCCTGTTTTCTAGAACTTTCTATGATGCAAAATCT GTTGATGAATTTCCTGAAATAAACCGAGCCTCAATTCGACAGGATCACAAGGGGAGATTCTATGAAGAC TTTTACAAAGTGGTGGTGCAGAATGAGAGAAGAGAAGAATGGACTTCACTTCTCGTAACCATTAAAAAA CTCTTCATCCAGTATCCAGTGTTGGTGCGACTGGAACAGGCAGAGGGCTTTCCTCAAGGAGATAATTCT ACCTCAGCACAAGGAAACTACCTGGAGGCCATCAATCTGTCATTCAATGTGTTTGATAAGCACTACATC AACCGCAACTTTGACCGAACTGGGCAGATGTCAGTGGTGATCACGCCCGGGGTGGGTGTCTTTGAAGTG GACCGCCTACTCATGATCCTGACCAAGCAGCGGATGATAGATAATGGAATTGGTGTGGATTTGGTGTGC ATGGGAGAGCAACCGTTACATGCTGTCCCATTGTTCAAGCTCCATAATCGGAGTGCTCCCCGTGATTCT CGTCTGGGCGATGACTATAATATCCCTCACTGGATAAACCACAGTTTCTACACATCCAAAAGCCAGCTC TTTTGTAATAGTTTCACCCCACGAATAAAACTGGCAGGAAAGAAGCCCGCCTCTGAGAAAGCAAAAAAT GGCCGTGATACATCTCTCGGGAGTCCAAAAGAATCTGAGAACGCCCTTCCCATCCAAGTAGATTATGAC GCCTATGACGCTCAAGTGTTCAGGCTGCCCGGCCCATCCCGGGCCCAGTGCCTCACCACCTGCAGATCT GTGCGAGAGCGAGAGAGTCACAGTCGAAAGAGTGCCAGCTCCTGTGATGTTTCATCCAGCCCTTCCCTA CCAAGCCGCACACTGCCCACTGAGGAAGTGAGGAGCCAGGCTTCTGACGACAGCTCCCTAGGCAAGAGT GCCAACATCCTGATGATCCCACACCCCCACCTGCACCAGTATGAAGTCAGCAGCTCCTTGGGATACACC AGCACTCGAGAGCACCTAGGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001007188 |
| Insert Size | 1680 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007188.2 |
| RefSeq Size | 2460 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 9681 |
| UniProt ID | O75140 |
| MW | 63.7 kDa |
| Gene Summary | This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2) lacks a large portion of the 3' coding region and has a different 3' structure compared to variant 4. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 4. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211462 | DEPDC5 (Myc-DDK-tagged)-Human DEP domain containing 5 (DEPDC5), transcript variant 2 |
CNY 4,160.00 |
|
| RC211462L1 | Lenti ORF clone of Human DEP domain containing 5 (DEPDC5), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC211462L2 | Lenti ORF clone of Human DEP domain containing 5 (DEPDC5), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC211462L3 | Lenti ORF clone of Human DEP domain containing 5 (DEPDC5), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC211462L4 | Lenti ORF clone of Human DEP domain containing 5 (DEPDC5), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG211462 | DEPDC5 (tGFP-tagged) - Human DEP domain containing 5 (DEPDC5), transcript variant 2 |
CNY 5,040.00 |
