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IF 2(Mt) (MTIF2) (NM_001005369) Human Untagged Clone
CAT＃: SC300928
MTIF2 (untagged)-Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 13,970.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300928 representing NM_001005369.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACCAGAAGCTACTGAAGTTGGAGAACTTGCTACGATTTCACACTATTTATAGGCAACTGCACAGT CTGTGTCAAAGAAGAGCATTAAGACAGTGGAGGCATGGGTTTTCATCTGCTTACCCTGTGTGGACAGCT CAACTGTGTGCCTGGCCCTGGCCAACAGATGTGCTCACTGGGGCTGCTTTATCTCAGTATAGGCTTCTA GTAACAAAAAAGGAAGAAGGACCATGGAAATCTCAGTTATCTTCAACAAAATCTAAAAAGGTGGTAGAA GTATGGATTGGAATGACTATTGAGGAACTGGCCAGGGCAATGGAAAAAAACACAGATTATGTATATGAA GCTTTATTGAACACTGATATTGACATAGATTCACTGGAAGCAGACTCACATTTAGATGAAGTCTGGATC AAAGAAGTGATAACGAAGGCAGGGATGAAGTTAAAGTGGAGTAAATTAAAACAGGACAAAGTCAGAAAA AATAAAGATGCTGTAAGAAGGCCCCAGGCAGATCCAGCTTTATTAACCCCAAGGTCCCCAGTTGTTACT ATAATGGGCCATGTTGATCACGGGAAAACGACATTACTTGACAAATTTCGAAAAACTCAAGTGGCAGCA GTGGAAACTGGAGGCATCACTCAGCACATTGGTGCCTTTCTTGTCTCTCTGCCTTCTGGGGAAAAGATA ACTTTTCTTGATACTCCAGGACATGCTGCTTTCTCAGCAATGAGAGCCAGAGGTGCTCAGGTCACTGAC ATTGTCGTATTGGTTGTAGCTGCAGATGATGGAGTGATGAAACAAACTGTAGAATCTATTCAGCATGCC AAAGATGCACAGGTTCCTATTATCCTTGCCGTAAATAAATGTGACAAAGCTGAGGCTGATCCTGAGAAA GTGAAAAAAGAGCTGCTGGCTTACGATGTGGTATGTGAAGATTATGGAGGTGATGTTCAAGCAGTGCCT GTCTCCGCACTTACGGGCGATAATCTGATGGCTTTGGCAGAAGCAACAGTTGCTCTTGCAGAAATGTTA GAATTGAAAGCAGATCCCAATGGTCCAGTGGAAGGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGA GGTCTTGTTACTACAGCTATAATTCAAAGAGGAACTTTAAGAAAAGGCTCTGTTCTGGTTGCTGGAAAA TGTTGGGCAAAAGTACGCTTAATGTTTGATGAAAATGGAAAAACAATTGATGAGGCCTATCCCAGCATG CCAGTGGGAATTACAGGCTGGAGAGACCTTCCTTCTGCAGGAGAAGAAATTCTTGAAGTAGAATCTGAG CCAAGGGCACGTGAAGTTGTTGACTGGAGGAAATATGAACAAGAACAGGAGAAAGGTCAGGAGGATCTG AAAATAATAGAAGAAAAGCGAAAGGAACACAAAGAAGCACATCAGAAAGCCCGTGAGAAGTATGGCCAT CTACTGTGGAAGAAGAGATCAATTCTACGGTTTTTAGAAAGAAAAGAACAAATACCCTTAAAGCCAAAA GAGAAAAGGGAAAGAGATTCAAATGTACTTTCTGTGATTATTAAAGGTGATGTTGATGGTTCTGTTGAG GCCATTTTGAACATTATAGATACCTATGATGCTTCACACGAGTGTGAACTAGAATTAGTACATTTTGGA GTGGGTGATGTAAGTGCAAATGATGTTAACCTTGCTGAAACATTTGATGGTGTTATATATGGCTTTAAT GTGAATGCAGGCAATGTTATCCAACAGTCAGCTGCAAAAAAAGGAGTAAAAATTAAACTTCACAAAATA ATTTACCGTCTTGTTGAAGATTTGCAAGAGGAACTGAGCAGCAGATTACCCTGTGCTGTGGAAGAGCAC CCAGTAGGTGAGGCATCTATACTAGCTACCTTCTCTGTAACAGAAGGGAAGAAAAAAGTTCCTGTGGCT GGCTGCAGAGTCCAAAAGGGACAGTTAGAAAAACAAAAAAAATTTAAACTAACCCGTAATGGACATGTA ATTTGGAAGGGCTCATTAACCTCATTGAAACACCATAAAGATGACATTTCAATTGTCAAAACGGGAATG GATTGTGGTCTCAGTTTAGATGAAGACAATATGGAATTTCAAGTGGGAGACAGAATTGTTTGTTATGAA GAAAAGCAAATTCAAGCCAAGACTTCTTGGGATCCAGGATTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001005369 |
| Insert Size | 2184 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005369.1 |
| RefSeq Size | 2965 bp |
| RefSeq ORF | 2184 bp |
| Locus ID | 4528 |
| UniProt ID | P46199 |
| MW | 81.3 kDa |
| Gene Summary | During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. [provided by RefSeq, Mar 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215473 | MTIF2 (Myc-DDK-tagged)-Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,320.00 |
|
| RC215473L3 | Lenti ORF clone of Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC215473L4 | Lenti ORF clone of Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG215473 | MTIF2 (tGFP-tagged) - Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,370.00 |
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