USP16 (NM_001001992) Human Untagged Clone

CAT#: SC300379

USP16 (untagged)-Human ubiquitin specific peptidase 16 (USP16), transcript variant 2



  "NM_001001992" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
USP16 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms UBP-M; UBPM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC300379 representing NM_001001992.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGATTCCTCTGAAACTTTAGAACCTGTG
TGCAGACACATTAGAAAAGGATTGGAACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAAT
ATCTGCCAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAACAGAAGAAAAGCCT
TCAGTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCTGTGGCAGAAATTCTCAGGAGCAGCATGCCTTG
AAGCACTATCTGACGCCAAGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATGG
TGTTACGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGGGTCAAGTGGTTGATTATGTC
AGAAAACAAGCCAGCATTACAACTCCAAAGCCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAA
AAATTAGAAAAAGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAGAGAATCCT
CCCATGAATTCTCCTTGCCAAATAACCGTGAAAGGACTCAGTAATTTGGGAAACACATGTTTCTTCAAT
GCAGTTATGCAGAACTTGTCACAAACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGA
ACAATTGTAAAAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATAAACCTTGAGCCTCCA
GGCCCTCTTACTTTAGCCATGAGCCAGTTTCTTAATGAGATGCAAGAGACCAAAAAGGGGGTTGTGACA
CCGAAAGAACTCTTTTCTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGACAGC
CAGGAGCTGCTTCGCTACTTATTGGATGGGATGAGAGCAGAAGAACACCAAAGAGTGAGTAAAGGAATA
CTTAAAGCATTTGGTAATTCTACTGAAAAGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAG
AAGAAAAAATCAATGCCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTATGATCATGTGT
GATCAATGCAGAACTGTCTCCTTGGTTCATGAATCTTTCCTTGATTTGTCCCTCCCAGTTTTAGATGAT
CAGAGTGGTAAGAAAAGTGTAAATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGT
GAGGAAGAAAAAGATAACGACAGTTACATAAAAGAGAGAAGTGATATTCCTTCTGGAACAAGTAAGCAC
TTACAGAAAAAAGCAAAGAAACAAGCCAAAAAGCAAGCCAAGAACCAACGAAGACAACAAAAAATTCAA
GGAAAAGTTCTTCATTTAAATGATATTTGTACTATTGACCATCCTGAAGACAGTGAATATGAAGCTGAA
ATGTCACTTCAAGGAGAAGTAAATATTAAATCCAACCATATTTCACAAGAGGGTGTTATGCATAAAGAA
TATTGTGTCAACCAGAAAGATTTGAATGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAA
TCCACAGAGGAAGTAGATATGAAAAATATCAACATGGATAATGATCTGGAGGTTTTAACATCTTCTCCC
ACTAGGAATTTAAATGGTGCCTACCTAACGGAAGGGAGCAATGGAGAAGTGGACATTTCCAATGGTTTC
AAAAACCTAAATTTGAATGCTGCTCTTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCAT
ACTCCTGGAACAAAGGTGTATGAGGTTGTAAATGAAGATCCAGAAACTGCTTTCTGTACTCTTGCAAAC
AGGGAAGTTTTCAATACTGATGAGTGTTCAATCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAA
CTTCGAGATGCGAATAAACTGCTTTGTGAAGTATGCACACGGAGACAGTGTAATGGACCAAAGGCAAAT
ATAAAAGGTGAAAGGAAGCATGTTTACACCAATGCCAAAAAGCAGATGCTAATTTCTCTTGCTCCTCCT
GTTCTTACTCTTCATTTAAAGAGATTTCAGCAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATA
AAGTTTCCGGAAATCTTAGATTTGGCTCCTTTTTGCACCCTTAAATGTAAGAATGTTGCAGAAGAAAAT
ACAAGGGTACTCTATTCCTTATATGGAGTTGTTGAACACAGTGGTACTATGAGGTCGGGGCATTACACT
GCCTATGCCAAGGCAAGAACCGCAAATAGTCATCTCTCTAATCTTGTTCTTCACGGTGATATTCCACAA
GATTTTGAAATGGAATCAAAAGGGCAGTGGTTTCACATCAGCGACACACATGTGCAAGCTGTGCCTACA
ACTAAAGTACTAAACTCACAAGCGTACCTCCTATTTTATGAGAGAATACTGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001001992
Insert Size 2469 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001001992.1
RefSeq Size 2986 bp
RefSeq ORF 2469 bp
Locus ID 10600
UniProt ID Q9Y5T5
Protein Families Protease
MW 93.5 kDa
Gene Summary This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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