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COP1 (RFWD2) (NM_001001740) Human Untagged Clone
CAT＃: SC300304
RFWD2 (untagged)-Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 2
CNY 13,590.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CFAP78; FAP78; RFWD2; RNF200 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300304 representing NM_001001740.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCGGCCTCCTCG GTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTTCCGTGGCGGTTTCGGCGGCAGCG CTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGCCTCGGGGGCCCGGTGCGGCCTGTGTTG GTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGGGCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCG GCCAGGCCCAGCGCCGGCGTAGGAGGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTC CTCGCCCCCCTCTGCAACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATC TGCTTTGATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATTCAT CAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATTGACCATCTGTAT CCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTTGAGGAAAAGAGGTTCAAATTG GACCACTCAAATGGCCACAGGTGGCAGATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGAT TTGGCCAATGTCAATCTTATGTTGGAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACAT GCAGCCCAACTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGGAAATGAGT GGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGT AGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAG CCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAG TGTTACTTTTCTACAAGGATGTCTCGTATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTT CAGGAATGCTTGTCCAAGTTTACTCGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGT GATCTCTATAATGGTTCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATT GCTGGAGTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCAT TACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAAC CTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAG GTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCT TCAGGTTCTGATGATGCAAAAGTGAAGCTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAG GCAAAGGCTAATGTGTGCTGTGTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCA GATCACTGTGTCCACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGT AAAGCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTA AAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTT GTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTAT AAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGA AAAGAAGATGATACAAATGAATTTGTTAGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAAT GTGCTGATTGCTGCTAACAGTCAGGGTACAATTAAGGTGCTAGAATTGGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001001740 |
| Insert Size | 2124 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001001740.3 |
| RefSeq Size | 2734 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 64326 |
| UniProt ID | Q8NHY2 |
| Protein Pathways | p53 signaling pathway, Ubiquitin mediated proteolysis |
| MW | 77.7 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (b, also known as delta24) is shorter, compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211483 | RFWD2 (Myc-DDK-tagged)-Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 2 |
CNY 5,176.00 |
|
| RC211483L3 | Lenti ORF clone of Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC211483L4 | Lenti ORF clone of Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG211483 | RFWD2 (tGFP-tagged) - Human ring finger and WD repeat domain 2 (RFWD2), transcript variant 2 |
CNY 6,370.00 |
