Intersectin 1 (ITSN1) (NM_001001132) Human Untagged Clone

CAT#: SC300159

ITSN1 (untagged)-Human intersectin 1 (SH3 domain protein) (ITSN1), transcript variant 2



  "NM_001001132" in other vectors (4)

CNY 23,560.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-Itsn1 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ITSN; SH3D1A; SH3P17
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_001001132, the custom clone sequence may differ by one or more nucleotides
ATGGCTCAGTTTCCAACACCTTTTGGTGGCAGCCTGGATATCTGGGCCATAACTGTAGAG
GAAAGAGCGAAGCATGATCAGCAGTTCCATAGTTTAAAGCCAATATCTGGATTCATTACT
GGTGATCAAGCTAGAAACTTTTTTTTTCAATCTGGGTTACCTCAACCTGTTTTAGCACAG
ATATGGGCACTAGCTGACATGAATAATGATGGAAGAATGGATCAAGTGGAGTTTTCCATA
GCTATGAAACTTATCAAACTGAAGCTACAAGGATATCAGCTACCCTCTGCACTTCCCCCT
GTCATGAAACAGCAACCAGTTGCTATTTCTAGCGCACCAGCATTTGGTATGGGAGGTATC
GCCAGCATGCCACCGCTTACAGCTGTTGCTCCAGTGCCAATGGGATCCATTCCAGTTGTT
GGAATGTCTCCAACCCTAGTATCTTCTGTTCCCACAGCAGCTGTGCCCCCCCTGGCTAAC
GGGGCTCCCCCTGTTATACAACCTCTGCCTGCATTTGCTCATCCTGCAGCCACATTGCCA
AAGAGTTCTTCCTTTAGTAGATCTGGTCCAGGGTCACAACTAAACACTAAATTACAAAAG
GCACAGTCATTTGATGTGGCCAGTGTCCCACCAGTGGCAGAGTGGGCTGTTCCTCAGTCA
TCAAGACTGAAATACAGGCAATTATTCAATAGTCATGACAAAACTATGAGTGGACACTTA
ACAGGTCCCCAAGCAAGAACTATTCTTATGCAGTCAAGTTTACCACAGGCTCAGCTGGCT
TCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTTACAGCAGAGGAATTTATC
CTGGCAATGCACCTCATTGATGTAGCTATGTCTGGCCAACCACTGCCACCTGTCCTGCCT
CCAGAATACATTCCACCTTCTTTTAGAAGAGTTCGATCTGGCAGTGGTATATCTGTCATA
AGCTCAACATCTGTAGATCAGAGGCTACCAGAGGAACCAGTTTTAGAAGATGAACAACAA
CAATTAGAAAAGAAATTACCTGTAACGTTTGAAGATAAGAAGCGGGAGAACTTTGAACGT
GGCAACCTGGAACTGGAGAAACGAAGGCAAGCTCTCCTGGAACAGCAGCGCAAGGAGCAG
GAGCGCCTGGCCCAGCTGGAGCGGGCGGAGCAGGAGAGGAAGGAGCGTGAGCGCCAGGAG
CAAGAGCGCAAAAGACAACTGGAACTGGAGAAGCAACTGGAAAAGCAGCGGGAGCTAGAA
CGGCAGAGAGAGGAGGAGAGGAGGAAAGAAATTGAGAGGCGAGAGGCTGCAAAACGGGAA
CTTGAAAGGCAACGACAACTTGAGTGGGAACGGAATCGAAGGCAAGAACTACTAAATCAA
AGAAACAAAGAACAAGAGGACATAGTTGTACTGAAAGCAAAGAAAAAGACTTTGGAATTT
GAATTAGAAGCTCTAAATGATAAAAAGCATCAACTAGAAGGGAAACTTCAAGATATCAGA
TGTCGATTGACCACCCAAAGGCAAGAAATTGAGAGCACAAACAAATCTAGAGAGTTGAGA
ATTGCCGAAATCACCCATCTACAGCAACAATTACAGGAATCTCAGCAAATGCTTGGAAGA
CTTATTCCAGAAAAACAGATACTCAATGACCAATTAAAACAAGTTCAGCAGAACAGTTTG
CACAGAGATTCACTTGTTACACTTAAAAGAGCCTTAGAAGCAAAAGAACTAGCTCGGCAG
CACCTACGAGACCAACTGGATGAAGTGGAGAAAGAAACTAGATCAAAACTACAGGAGATT
GATATTTTCAATAATCAGCTGAAGGAACTAAGAGAAATACACAATAAGCAACAACTCCAG
AAGCAAAAGTCCATGGAGGCTGAACGACTGAAACAGAAAGAACAAGAACGAAAGATCATA
GAATTAGAAAAACAAAAAGAAGAAGCCCAAAGACGAGCTCAGGAAAGGGACAAGCAGTGG
CTGGAGCATGTGCAGCAGGAGGACGAGCATCAGAGACCAAGAAAACTCCACGAAGAGGAA
AAACTGAAAAGGGAGGAGAGTGTCAAAAAGAAGGATGGCGAGGAAAAAGGCAAACAGGAA
GCACAAGACAAGCTGGGTCGGCTTTTCCATCAACACCAAGAACCAGCTAAGCCAGCTGTC
CAGGCACCCTGGTCCACTGCAGAAAAAGGTCCACTTACCATTTCTGCACAGGAAAATGTA
AAAGTGGTGTATTACCGGGCACTGTACCCCTTTGAATCCAGAAGCCATGATGAAATCACT
ATCCAGCCAGGAGACATAGTCATGGTTAAAGGGGAATGGGTGGATGAAAGCCAAACTGGA
GAACCCGGCTGGCTTGGAGGAGAATTAAAAGGAAAGACAGGGTGGTTCCCTGCAAACTAT
GCAGAGAAAATCCCAGAAAATGAGGTTCCCGCTCCAGTGAAACCAGTGACTGATTCAACA
TCTGCCCCTGCCCCCAAACTGGCCTTGCGTGAGACCCCCGCCCCTTTGGCAGTAACCTCT
TCAGAGCCCTCCACGACCCCTAATAACTGGGCCGACTTCAGCTCCACGTGGCCCACCAGC
ACGAATGAGAAACCAGAAACGGATAACTGGGATGCATGGGCAGCCCAGCCCTCTCTCACC
GTTCCAAGTGCCGGCCAGTTAAGGCAGAGGTCCGCCTTTACTCCAGCCACGGCCACTGGC
TCCTCCCCGTCTCCTGTGCTAGGCCAGGGTGAAAAGGTGGAGGGGCTACAAGCTCAAGCC
CTATATCCTTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAATGATGTCATC
ACCGTCCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGG
TTCCCCAAGTCTTACGTGAAACTCATTTCAGGGCCCATAAGGAAGTCTACAAGCATGGAT
TCTGGTTCTTCAGAGAGTCCTGCTAGTCTAAAGCGAGTAGCCTCTCCAGCAGCCAAGCCG
GTCGTTTCGGGAGAAGAATTTATTGCCATGTACACTTACGAGAGTTCTGAGCAAGGAGAT
TTAACCTTTCAGCAAGGGGATGTGATTTTGGTTACCAAGAAAGATGGTGACTGGTGGACA
GGAACAGTGGGCGACAAGGCCGGAGTCTTCCCTTCTAACTATGTGAGGCTTAAAGATTCA
GAGGGCTCTGGAACTGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAG
GTTATTGCCTCATACACCGCCACCGGCCCCGAGCAGCTCACTCTCGCCCCTGGTCAGCTG
ATTTTGATCCGAAAAAAGAACCCAGGTGGATGGTGGGAAGGAGAGCTGCAAGCACGTGGG
AAAAAGCGCCAGATAGGCTGGTTCCCAGCTAATTATGTAAAGCTTCTAAGCCCTGGGACG
AGCAAAATCACTCCAACAGAGCCACCTAAGTCAACAGCATTAGCGGCAGTGTGCCAGGTG
ATTGGGATGTACGACTACACCGCGCAGAATGACGATGAGCTGGCCTTCAACAAGGGCCAG
ATCATCAACGTCCTCAACAAGGAGGACCCTGACTGGTGGAAAGGAGAAGTCAATGGACAA
GTGGGGCTCTTCCCATCCAATTATGTGAAGCTGACCACAGACATGGACCCAAGCCAGCAA
TGA
Restriction Sites Please inquire     
ACCN NM_001001132
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001001132.1, NP_001001132.1
RefSeq Size 5451 bp
RefSeq ORF 3663 bp
Locus ID 6453
UniProt ID Q15811
Gene Summary The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (ITSN-s) is shorter at the C-terminus compared to isoform ITSN-l.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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