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PTCH1 (NM_000264) Human Untagged Clone
CAT＃: SC300040
PTCH1 (untagged)-Human patched 1 (PTCH1), transcript variant 1b
CNY 14,864.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCNS; NBCCS; PTC; PTC1; PTCH |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000264 edited
CAGCGGCTGGTCTGTCAACCGGAGCCCGAGCCCGAGCAGCCTGCGGCCAGCAGCGTCCTC GCAAGCCGAGCGCCCAGGCGCGCCAGGAGCCCGCAGCAGCGGCAGCAGCGCGCCGGGCCG CCCGGGAAGCCTCCGTCCCCGCGGCGGCGGCGGCGGCGGCGGCAACATGGCCTCGGCTGG TAACGCCGCCGAGCCCCAGGACCGCGGCGGCGGCGGCAGCGGCTGTATCGGTGCCCCGGG ACGGCCGGCTGGAGGCGGGAGGCGCAGACGGACGGGGGGGCTGCGCCGTGCTGCCGCGCC GGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGCTCTGGAGCAGAT TTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAGAG ACTCTTATTTAAACTGGGTTGTTACATTCAAAAAAACTGCGGCAAGTTCTTGGTTGTGGG CCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAAAGCAGCGAACCTCGAGACCAACGT GGAGGAGCTGTGGGTGGAAGTTGGAGGACGAGTAAGTCGTGAATTAAATTATACTCGCCA GAAGATTGGAGAAGAGGCTATGTTTAATCCTCAACTCATGATACAGACCCCTAAAGAAGA AGGTGCTAATGTCCTGACCACAGAAGCGCTCCTACAACACCTGGACTCGGCACTCCAGGC CAGCCGTGTCCATGTATACATGTACAACAGGCAGTGGAAATTGGAACATTTGTGTTACAA ATCAGGAGAGCTTATCACAGAAACAGGTTACATGGATCAGATAATAGAATATCTTTACCC TTGTTTGATTATTACACCTTTGGACTGCTTCTGGGAAGGGGCGAAATTACAGTCTGGGAC AGCATACCTCCTAGGTAAACCTCCTTTGCGGTGGACAAACTTCGACCCTTTGGAATTCCT GGAAGAGTTAAAGAAAATAAACTATCAAGTGGACAGCTGGGAGGAAATGCTGAATAAGGC TGAGGTTGGTCATGGTTACATGGACCGCCCCTGCCTCAATCCGGCCGATCCAGACTGCCC CGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTGATATGGCCCTTGTTTTGAATGG TGGATGTCATGGCTTATCCAGAAAGTATATGCACTGGCAGGAGGAGTTGATTGTGGGTGG CACAGTCAAGAACAGCACTGGAAAACTCGTCAGCGCCCATGCCCTGCAGACCATGTTCCA GTTAATGACTCCCAAGCAAATGTACGAGCACTTCAAGGGGTACGAGTATGTCTCACACAT CAACTGGAACGAGGACAAAGCGGCAGCCATCCTGGAGGCCTGGCAGAGGACATATGTGGA GGTGGTTCATCAGAGTGTCGCACAGAACTCCACTCAAAAGGTGCTTTCCTTCACCACCAC AACCCTGGACGACATCCTGAAATCCTTCTCTGACGTCAGTGTCATCCGCGTGGCCAGCGG CTACTTACTCATGCTCGCCTATGCCTGTCTAACCATGCTGCGCTGGGACTGCTCCAAGTC CCAGGGTGCCGTGGGGCTGGCTGGCGTCCTGCTGGTTGCACTGTCAGTGGCTGCAGGACT GGGCCTGTGCTCATTGATCGGAATTTCCTTTAACGCTGCAACAACTCAGGTTTTGCCATT TCTCGCTCTTGGTGTTGGTGTGGATGATGTTTTTCTTCTGGCCCACGCCTTCAGTGAAAC AGGACAGAATAAAAGAATCCCTTTTGAGGACAGGACCGGGGAGTGCCTGAAGCGCACAGG AGCCAGCGTGGCCCTCACGTCCATCAGCAATGTCACAGCCTTCTTCATGGCCGCGTTAAT CCCAATTCCCGCTCTGCGGGCGTTCTCCCTCCAGGCAGCGGTAGTAGTGGTGTTCAATTT TGCCATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCATGGATTTATATCGACGCGAGGA CAGGAGACTGGATATTTTCTGCTGTTTTACAAGCCCCTGCGTCAGCAGAGTGATTCAGGT TGAACCTCAGGCCTACACCGACACACACGACAATACCCGCTACAGCCCCCCACCTCCCTA CAGCAGCCACAGCTTTGCCCATGAAACGCAGATTACCATGCAGTCCACTGTCCAGCTCCG CACGGAGTACGACCCCCACACGCACGTGTACTACACCACCGCTGAGCCGCGCTCCGAGAT CTCTGTGCAGCCCGTCACCGTGACACAGGACACCCTCAGCTGCCAGAGCCCAGAGAGCAC CAGCTCCACAAGGGACCTGCTCTCCCAGTTCTCCGACTCCAGCCTCCACTGCCTCGAGCC CCCCTGTACGAAGTGGACACTCTCATCTTTTGCTGAGAAGCACTATGCTCCTTTCCTCTT GAAACCAAAAGCCAAGGTAGTGGTGATCTTCCTTTTTCTGGGCTTGCTGGGGGTCAGCCT TTATGGCACCACCCGAGTGAGAGACGGGCTGGACCTTACGGACATTGTACCTCGGGAAAC CAGAGAATATGACTTTATTGCTGCACAATTCAAATACTTTTCTTTCTACAACATGTATAT AGTCACCCAGAAAGCAGACTACCCGAATATCCAGCACTTACTTTACGACCTACACAGGAG TTTCAGTAACGTGAAGTATGTCATGTTGGAAGAAAACAAACAGCTTCCCAAAATGTGGCT GCACTACTTCAGAGACTGGCTTCAGGGACTTCAGGATGCATTTGACAGTGACTGGGAAAC CGGGAAAATCATGCCAAACAATTACAAGAATGGATCAGACGATGGAGTCCTTGCCTACAA ACTCCTGGTGCAAACCGGCAGCCGCGATAAGCCCATCGACATCAGCCAGTTGACTAAACA GCGTCTGGTGGATGCAGATGGCATCATTAATCCCAGCGCTTTCTACATCTACCTGACGGC TTGGGTCAGCAACGACCCCGTCGCGTATGCTGCCTCCCAGGCCAACATCCGGCCACACCG ACCAGAATGGGTCCACGACAAAGCCGACTACATGCCTGAAACAAGGCTGAGAATCCCGGC AGCAGAGCCCATCGAGTATGCCCAGTTCCCTTTCTACCTCAACGGCTTGCGGGACACCTC AGACTTTGTGGAGGCAATTGAAAAAGTAAGGACCATCTGCAGCAACTATACGAGCCTGGG GCTGTCCAGTTACCCCAACGGCTACCCCTTCCTCTTCTGGGAGCAGTACATCGGCCTCCG CCACTGGCTGCTGCTGTTCATCAGCGTGGTGTTGGCCTGCACATTCCTCGTGTGCGCTGT CTTCCTTCTGAACCCCTGGACGGCCGGGATCATTGTGATGGTCCTGGCGCTGATGACGGT CGAGCTGTTCGGCATGATGGGCCTCATCGGAATCAAGCTCAGTGCCGTGCCCGTGGTCAT CCTGATCGCTTCTGTTGGCATAGGAGTGGAGTTCACCGTTCACGTTGCTTTGGCCTTTCT GACGGCCATCGGCGACAAGAACCGCAGGGCTGTGCTTGCCCTGGAGCACATGTTTGCACC CGTCCTGGATGGCGCCGTGTCCACTCTGCTGGGAGTGCTGATGCTGGCGGGATCTGAGTT CGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCACCATCCTCGGCGTTCT CAATGGGCTGGTTTTGCTTCCCGTGCTTTTGTCTTTCTTTGGACCATATCCTGAGGTGTC TCCAGCCAACGGCTTGAACCGCCTGCCCACACCCTCCCCTGAGCCACCCCCCAGCGTGGT CCGCTTCGCCATGCCGCCCGGCCACACGCACAGCGGGTCTGATTCCTCCGACTCGGAGTA TAGTTCCCAGACGACAGTGTCAGGCCTCAGCGAGGAGCTTCGGCACTACGAGGCCCAGCA GGGCGCGGGAGGCCCTGCCCACCAAGTGATCGTGGAAGCCACAGAAAACCCCGTCTTCGC CCACTCCACTGTGGTCCATCCCGAATCCAGGCATCACCCACCCTCGAACCCGAGACAGCA GCCCCACCTGGACTCAGGGTCCCTGCCTCCCGGACGGCAAGGCCAGCAGCCCCGCAGGGA CCCCCCCAGAGAAGGCTTGTGGCCACCCCTCTACAGACCGCGCAGAGACGCTTTTGAAAT TTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGCCCGCTGGGGCCCTCGCGGGGCCCG TTCTCACAACCCTCGGAACCCAGCGTCCACTGCCATGGGCAGCTCCGTGCCCGGCTACTG CCAGCCCATCACCACTGTGACGGCTTCTGCCTCCGTGACTGTCGCCGTGCACCCGCCGCC TGTCCCTGGGCCTGGGCGGAACCCCCGAGGGGGACTCTGCCCAGGCTACCCTGAGACTGA CCACGGCCTGTTTGAGGACCCCCACGTGCCTTTCCACGTCCGGTGTGAGAGGAGGGATTC GAAGGTGGAAGTCATTGAGCTGCAGGACGTGGAATGCGAGGAGAGGCCCCGGGGAAGCAG CTCCAACTGAGGGTGATTAAAATCTGAAGCAAAGAGGCCAAAGATTGGAAACCCCCCACC CCCACCTCTTTCCAGAACTGCTTGAAGAGAACTGGTTGGAGTTATGGAAAAGATGCCCTG TGCCAGGACAGCAGTTCATTGTTACTGTAACCGATTGTATTATTTTGTTAAATATTTCTA TAAATATTTAAGAGATGTACACATGTGTAATATAGGAAGGAAGGATGTAAAGTGGTATGA TCTGGGGCTTCTCCACTCCTGCCCCAGAGTGTGGAGGCCACAGTGGGGCCTCTCCGTATT TGTGCATTGGGCTCCGTGCCACAACCAAGCTTGTCGACTCTAGATTGCGGCCGCGGTCAT AGCTGTTTCCTGAACAGATCCCGGGTGGCAG >OriGene 5' read for NM_000264 unedited
CGTGACATTTGTATACGATCATATAGGCGGCCGCGNATTCAAATCTGGTACCGATATCTG CAGAATTCGCCCTTCAGCGGCTGGTCTGTCAACCGGAGCCCGAGCCCGAGCAGCCTGCGG CCAGCAGCGTCCTCGCAAGCCGAGCGCCCAGGCGCGCCAGGAGCCCGCAGCAGCGGCAGC AGCGCGCCGGGCCGCCCGGGAAGCCTCCGTCCCCGCGGCGGCGGCGGCGGCGGCGGCAAC ATGGCCTCGGCTGGTAACGCCGCCGAGCCCCAGGACCGCGGCGGCGGCGGCAGCGGCTGT ATCGGTGCCCCGGGACGGCCGGCTGGAGGCGGGAGGCGCAGACGGACGGGGGGGCTGCGC CGTGCTGCCGCGCCGGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTC GCTCTGGAGCAGATTTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGA GCGAAGTTTCAGAGACTCTTATTTAAACTGGGTTGTTACATTCAAAAAAACTGCGGCAAG TTCTTGGTTGTGGGCCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAAAGCAGCGAAC CTCGAGACCAACGTGGAGGAGCTGTGGGTGGAAATTGGAGGACGAGTAAGTCCTGAATTA AAATATACTTGCCAGAAGATTGGAAAAAAGGCTATAGTTAAATCCTTCAACTCATGATAC AAAACCCCTAAAAAAAAAGGTGGCAAATGTCCTGGACCAAAAAAGCCGCTCCTTACAAAA ACTGGGATTTGGCACTTCAAGGCCAGCCGTGGCCCTGGTTACCTGGTAAACAAGGCCGG >OriGene 3' read for NM_000264 unedited
ACTATTGGTAGAGGTCGCTCTGCAGGGCCAGGNCGNAGCACTGGGGNAGGGTCACAGGCT GCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAG CTTGGTTGTGGCACGGAGCCCAATGCACAAATACGGAGAGGCCCCACTGTGGCCTCCACA CTCTGGGGCAGGAGTGGAGAAGCCCCAGATCATACCACTTTACATCCTTCCTTCCTATAT TACACATGTGTACATCTCTTAAATATTTATAGAAATATTTAACAAAATAATACAATCGGT TACAGTAACAATGAACTGCTGTCCTGGCACAGGGCATCTTTTCCATAACTCCAACCAGTT CTCTTCAAGCAGTTCTGGAAAGAGGTGGGGGTGGGGGGTTTCCAATCTTTGGCCTCTTTG CTTCAGATTTTAATCACCCTCAGTTGGAGCTGCTTCCCCGGGGCCTCTCCTCGCATTCCA CGTCCTGCAGCTCAATGACTTCCACCTTCGCCGTGTCCACTCTGCTGGGAGTGCTGATGC TGGCGGGATCTGAATTCGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCA CCATCCTCCGCGTTCTCAATGAGGCTGGTTTTGCTTCCCGTGCTTTTGTCCTTCCTTTGG ACCATATCCTGAGGTGTCTCCCGCCCAAGGCTTGAACCGGCTGGCCACACCCCTCCCTTG AACCCACCCCCAAGCGTGGTCCCGGTTTAACCATGCCCGGCCCGGCCCCAACGCCACAGC GGGTTTTGATCTCCTCCGACTTCGGAATTTAGTTTCCCCAGGAAAAAAGTGTACAGGACC TAATCAAAGGAACTTCTTGGAACTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_000264 |
| Insert Size | 4800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000264.2, NP_000255.1 |
| RefSeq Size | 6825 bp |
| RefSeq ORF | 4344 bp |
| Locus ID | 5727 |
| UniProt ID | Q13635 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
| Gene Summary | This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1b) encodes the longest isoform (L). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216999 | PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,840.00 |
|
| RC216999L1 | Lenti-ORF clone of PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 18,240.00 |
|
| RC216999L2 | Lenti-ORF clone of PTCH1 (mGFP-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 18,240.00 |
|
| RC216999L3 | Lenti-ORF clone of PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,680.00 |
|
| RC216999L4 | Lenti-ORF clone of PTCH1 (mGFP-tagged)-Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,680.00 |
|
| RG216999 | PTCH1 (tGFP-tagged) - Human patched 1 (PTCH1), transcript variant 1b |
CNY 15,200.00 |
