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SPART (NM_015087) Human Untagged Clone
CAT＃: SC128284
SPG20 (untagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPG20; TAHCCP1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_015087, the custom clone sequence may differ by one or more nucleotides
ATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAAATTAAGATCATCAGAGAAGCATATAAGAAGGCCT TTTTATTTGTTAACAAAGGTCTGAATACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTATAA GCAAGGAATAGGACACCTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTCCTGGG TGGGAATCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCTGGAAA TTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAGTTATATCCAGAATT TCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTTAGTTCAGCTCCTCAGCATGCTGAA GTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCAGTTGCTGCACCTGCTTCTCTGTCTTTACCATCAC AAAGTTGTCCAGCAGAAGCTCCTCCTGCTTATACTCCTCAAGCTGCTGAAGGTCACTACACTGTATCCTA TGGAACAGATTCTGGGGAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAGCCACCGCCT CTTGAGACCTTAGGGCTGGATGCAGATGAATTGATTTTGATACCAAATGGAGTACAGATTTTTTTTGTAA ATCCTGCAGGGGAGGTTAGTGCACCTTCGTATCCTGGGTACCTTCGAATTGTGAGGTTTTTGGATAATTC TCTCGATACGGTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTGTGACTGGTTATATCCTCTAGTTCCT GATAGATCTCCGGTTCTGAAATGTACTGCGGGAGCCTACATGTTTCCTGATACAATGCTACAAGCAGCAG GATGCTTTGTGGGGGTCGTCCTGTCCTCTGAGTTACCAGAGGATGATAGAGAGCTCTTTGAGGATCTGTT AAGGCAAATGTCTGACCTTCGGCTCCAGGCCAACTGGAACAGAGCAGAAGAAGAAAATGAATTCCAAATC CCTGGAAGAACTAGACCCTCCTCTGACCAACTAAAAGAAGCCTCTGGCACTGATGTGAAACAGTTGGACC AAGGCAATAAGGATGTACGTCATAAAGGAAAACGTGGAAAAAGGGCTAAAGATACTTCAAGTGAAGAAGT TAACCTGAGTCACATTGTACCATGTGAGCCAGTTCCAGAAGAAAAGCCAAAAGAATTACCTGAATGGAGT GAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTCCTGGGTGAGTTGGGGTTTAGTCAAAGGTGCTGAGA TTACTGGTAAGGCAATCCAGAAAGGTGCTTCTAAACTCCGAGAGCGGATTCAACCAGAAGAAAAACCCGT GGAAGTTAGTCCAGCTGTCACCAAGGGACTTTATATAGCGAAGCAAGCTACAGGAGGAGCAGCAAAAGTC AGTCAGTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGAAAAGAACTAGCTCCACATGTCA AGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAAAGATGGGAAATCTCCTCTGGATGG TGCTATGGTTGTAGCAGCAAGTAGTGTTCAAGGATTTTCAACTGTCTGGCAAGGATTGGAATGTGCAGCT AAATGCATCGTTAACAATGTTTCAGCAGAAACTGTACAAACTGTCAGATACAAATACGGATATAATGCAG GAGAAGCTACCCACCATGCGGTGGATTCTGCGGTCAATGTTGGCGTAACTGCCTACAATATTAACAACAT TGGTATCAAAGCAATGGTGAAGAAAACTGCAACACAAACAGGACACACTCTCCTTGAGGACTATCAGATA GTTGATAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAATGTCAACGTGAGAGGGGAGAAGGATGAGC AGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAATGA >OriGene 5' read for NM_015087 unedited
NGGGGGTTCGCGATATTTGTATACGACTTATATAGGCGGCCGCGCAATTCGCACGAGGGG AGTGGGAGCTGGTCCGTGCCGCGGCGGCCGCGCAGGGAGCTCTCGAGGCAACGCCGGGGC GCCCGAGGTCTGGAAGGCGCAGAAATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAA ATTAAGATCATCAGAGAAGCATATAAGAAGGCCTTTTTATTTGTTAACAAAGGTCTGAAT ACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTATAAGCAAGGAATAGGACAC CTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTCCTGGGTGGGAA TCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCTG GAAATTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAG TTATATCCAGAATTTCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTT AGTTCAGCTCCTCAGCATGCTGAAGTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCA GTTGCTGCACCTGCTTCTCTGTCTTTACCATCACAAAGTTGTCCAGCAGAAGCTCCTCCT GCTTATACTCCTCAAGCTGCTGAAGGTCACTACACTGTATCCTATGGAACAGATTCTGGG GAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAGCCACCGCCTCTTGAG ACCTTTAGGCTGGATGCAGATGAATTGATTNTGATACCAAATGGAGTACAGATTTTTTTT GTAAATCCTGCAGGGGGAGTTAGTGCACCTTCGTATCCTGGGTACCTCCGAATGTGAGGT TTTTGGATATNCTCTCGATACGGNTCTAACCGTNCTCCNGGNC >Forward primer walk for NM_015087 unedited
CGTATGGTTGCTTTCTAACCTCCGAGACGGATTAACCTGAAGAAAAACCCGTGGAAGCTT AGTCCAGCTTGTCACCAAGGGACTTTATATAGCGAAGCAAGCTACAGGAGGAGCAGCAAA AGTCAGTCAGTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGAAAAGAACT AGCTCCACATGTCAAGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAA AGATGGGAAATCTCCTCTGGATGGTGCTATGGTTGTAGCAGCAAGTAGTGTTCAAGGATT TTCAACTGTCTGGCAAGGATTGGAATGTGCAGCTAAATGCATCGTTAACAATGTTTCAGC AGAAACTGTACAAACTGTCAGATACAAATACGGATATAATGCAGGAGAAGCTACCCACCA TGCGGTGGATTCTGCGGTCAATGTTGGCGTAACTGCCTACAATATTAACAACATTGGTAT CAAAGCAATGGTGAAGAAAACTGCAACACAAACAGGACACACTCTCCTTGAGGACTATCA GATAGTTGATAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAATGTCAACGTGAGAGG GGAGAAGGATGAGCAGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAATGATGAAG TGCTGGGAATCACTTATACCAAAGCCTTATGAAATGGATGAAATTTTGTTAAATAGGCAA ATGTGGAATTCCTCACAGATTAACCAGTATTTTTTAAATGTATTCATTCCTACAAATTAA CTTTCATAAATTTTATGGCATGTCTTCTATTTAAAAGGAAAAGAATAAGTATTCTTGCAT CTGGCCTTAGAAATGTGAAGTTATATCTCAAGTTTATTTTTTTCCAGTGTAGCTAAATAT TTTGCAGTAAATAAAGCTGATAGTACATGTGTTGTTCAACTTGTTAAACCTAATATTGAA CTATTTTTTATAATCTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015087 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015087.3, NP_055902.1 |
| RefSeq Size | 4838 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 23111 |
| UniProt ID | Q8N0X7 |
| Domains | MIT |
| Gene Summary | This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3, and 4 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207971 | SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1 |
CNY 4,960.00 |
|
| RC207971L1 | Lenti ORF clone of Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1, Myc-DDK-tagged |
CNY 7,360.00 |
|
| RC207971L2 | Lenti ORF clone of Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207971L3 | Lenti ORF clone of Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207971L4 | Lenti ORF clone of Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG207971 | SPG20 (tGFP-tagged) - Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 1 |
CNY 6,560.00 |
