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HERC4 (NM_015601) Human Untagged Clone
CAT＃: SC128201
HERC4 (untagged)-Human hect domain and RLD 4 (HERC4), transcript variant 2
CNY 7,312.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128201 sequence for NM_015601 edited (data generated by NextGen Sequencing)
ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAA ATTGTACTAGAGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGA TGTGGACTCAGACATACTGTGTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGT AATGATCTAGGACAGCTAGGTCATGAAAAATCCAGAAAGAAACCAGAGCAGGTTGTTGCC CTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAGCTCATACGTTAGCGCTAAAT GACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAGCTTGGCCTGGTAGGA TCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAGATTGTA CAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGT TGGGGACAGAATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCA CCGCAGCTGCTTAAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCC CATAGTTTTGTACTCACCCTTTCTGGAGCTATCTTTGGATGGGGACGCAACAAGTTTGGT CAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTAATTTACTAAAGTCACTAAGA TCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCATACTGCTGCTCTAACCAAGGAA GGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGT CATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCT TGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGG CTTGGTGGTAATGGGCAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGTCCCTTTACT GTAAAAGGAAATTGGTACCCCTATAATGGGCAGTGTCTACCAGATATTGATTCTGAAGAA TATTTCTGTGTAAAAAGATTTTTCTCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGT CCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAAGCAGATCTGG ACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTCCTGTG GAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTT TTAGCTGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATG AATGCTGCTAGGCTTTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAG CAGGTGGCAGCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTACCTGAT GTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTCCCCTGATGAGTGATTCCAAC AATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAACCTAGAAAAGGCACCA CTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCT TCTGAAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTA CATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAA GTACAAGAATTGATAGACATAAGAAATGATTATATCAACTGGGTCCAACAGCAGGCCTAT GGAATGTTGGCAGATATCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCCCAA GCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAG GCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATCCCTGC TTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAA ACAAAGAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTG GATGCAGGAGGGGTGCGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCT AAATACGGCATGTTTAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGACA TTTGAAGACAGTGATTTGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCAATTTATAAT TGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAACTACTGAAAAAGAAG CCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAACAGTTA CTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTT GAAAACTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTT AACAAACAAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCA GTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTC CTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCATTGGAAATACAAATTATGAT TGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGAACATCCTACG ATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTG TTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTC ATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTT CTGGATCTTCCAAAATATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATT GATCACAATGAAGGCTTCAGTTTAATATAA Clone variation with respect to NM_015601.3 1011 c=>t;1099 a=>t >OriGene 5' read for NM_015601 unedited
GGGTGGCANAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCA CCTCCCCCTCTGCCTTCTCCTACCCAGTCTCTTCCCCAACTCTCCCCACCCCTGGTGCTC TCCGCCCCGCTTGCCTCCCGTTCTTCCCCGCCCGCTCCATCCGGGTCGCTGACGGCTGTC TCTGGACTGGACAGCAGTGGCGTCCGCTTTTCTCTAAGGAACCCGATAAGAACTCCATTT TCTGTTGATGTGGAGACATAGGGTACCTCCAACTTTCCGGGGTTTCAGAAGAGAATTTTT TATTGAAACTGGAAGACCAAAATAATTACAAGCATGTTGTGCTGGGGAAATGCATCCTTT GGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTAGAGCCCAGAAAAAGTGAC TTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGTGTTTGTT CTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAA AAATCCAGANAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTT TCATGTGGAGAAGCTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGT CTCGATTCTGATGGACAGCTTGGCCTGGTAGGATCAGAGGAATGCATCANAGTACCCAAA ATATNTAAAGTTTGTCAGATATCCAGATTGTACAGGTTGCTTGTGGTTACTATCATTCAC TTGCACTTTCTAAAGCAAGTGAAGTCCTTCTGTTGGGGACAGAATAAATATTGGCCAATT GGTTTAAGTACTGACTGTAAAAAGCAAACTTTACCGCAGCTGCTTTAGTCTTTGCCTGGG ATCCTTTCATGCAAGNTGCACCAGAAGGACCCTC >OriGene 3' genomic read for NM_015601 unedited
NGATCGATTTTTTCTTTTTTTTTTTAAATATTAAAACAATAAATCTTTATTAAACAGTTT TCACGGCATTTTAAAATAAATTTAAAAAATAAATATTTGCATTATAGCAGAAAGTCACAT GTATTGCATCAACTCACTTTTACAATTTAATGGCATGAGACAACTAAGCATCAGCACCAT AAAACTGTTAAGATTTGTTTCTTAAATTCAAGGAAATAGCTCTAAAGCACTGCAGCATCA ATAATTTTGAAAGCTATAACCCAGTGATTGGTGCTCATAGTAGCCAATGTTTCTTTTTAT TACTGGCAACTGTGGAGACAGAGTTCTTCCAGTACTAAATATTAATAAGTTCTCTGATCG ACAATCCCCCATCTATCAGTGTGTCAGGATTCACAAAGAAAAGAACTGCAGGTTATGTAG TATATTCCCTATCTGTGATTTAGGTTTGCACAAAGACACTTATTCTAGCAAAAAAGAAAA GATAGTGTTTCCACCATGCTACCTTTTTTTTCTTTTTATAGAGTCTCAGAGTAAGATTTA AATCATAATTTTTAACCTTTAAAAGTCTGTTCAAGTTCAATTACAACCATAGAAACTGTA CTGGTATCAGAACATAACTATTTTATTACAAAACTTAACATTATTTACAAAATGAAAAAA TAATCAAATGACTATTGCAGGCCAAAGTTAAAGGTTTTTTCACTCAATGATTGGAAGAAA ATTNAAGCATATTTTCATGCACTCACACCAGATCATTTCTGAAATATGCAAACTCTTAAA ATCATGTTAGTAAAACTTAATGTATTCATATACTTGCTAGTTTATTAGAAGAGGTCAAAA AAATCCATGGTCTGTACATAATATAACAAGTTGTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015601 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015601.2, NP_056416.2 |
| RefSeq Size | 3842 bp |
| RefSeq ORF | 3150 bp |
| Locus ID | 26091 |
| UniProt ID | Q5GLZ8 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208071 | HERC4 (Myc-DDK-tagged)-Human hect domain and RLD 4 (HERC4), transcript variant 2 |
CNY 7,304.00 |
|
| RC208071L1 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC208071L2 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RC208071L3 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC208071L4 | Lenti ORF clone of Human hect domain and RLD 4 (HERC4), transcript variant 2, mGFP tagged |
CNY 7,600.00 |
|
| RG208071 | HERC4 (tGFP-tagged) - Human hect domain and RLD 4 (HERC4), transcript variant 2 |
CNY 6,270.00 |
