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DHODH (NM_001361) Human Untagged Clone
CAT＃: SC128197
DHODH (untagged)-Human dihydroorotate dehydrogenase (DHODH), nuclear gene encoding mitochondrial protein
CNY 3,656.00
| Cited in 1 publication. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DHOdehase; POADS; URA1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128197 sequence for NM_001361 edited (data generated by NextGen Sequencing)
ATGGCGTGGAGACACCTGCAAAAGCGGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGA GGACTTCTCTTCGCCTCCTACCTGATGGCCACGGGAGATGAGCGTTTCTATGCTGAACAC CTGATGCCGACTCTGCAGGGGCTGCTGGACCCGGAGTCAGCCCACAGACTGGCTGTTCGC TTCACCTCCCTGGGGCTCCTTCCACGGGCCAGATTTCAAGACTCTGACATGCTGGAAGTG AGAGTTCTGGGCCATAAATTCCGAAATCCAGTAGGAATTGCTGCAGGATTTGACAAGCAT GGGGAAGCCGTGGACGGACTTTATAAGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTG ACTCCAAAACCTCAGGAAGGAAACCCTAGACCCAGAGTCTTCCGCCTCCCTGAGGACCAA GCTGTCATTAACAGGTATGGATTTAACAGTCACGGGCTTTCAGTGGTGGAACACAGGTTA CGGGCCAGACAGCAGAAGCAGGCCAAGCTCACAGAAGATGGACTGCCTCTGGGGGTCAAC TTGGGGAAGAACAAGACCTCAGTGGACGCCGCGGAGGACTACGCAGAAGGGGTGCGCGTA CTGGGCCCCCTGGCCGACTACCTGGTGGTGAATGTGTCCAGCCCCAACACTGCCGGGCTG CGGAGCCTTCAGGGAAAGGCCGAGCTGCGCCGCCTGCTGACCAAGGTGCTGCAGGAGAGG GATGGCTTGCGGAGAGTGCACAGGCCGGCAGTCCTGGTGAAGATCGCTCCTGACCTCACC AGCCAGGATAAGGAGGACATTGCCAGTGTGGTCAAAGAGTTGGGCATCGATGGGCTGATT GTTACGAACACCACCGTGAGTCGCCCTGCGGGCCTCCAGGGTGCCCTGCGCTCTGAAACA GGAGGGCTGAGTGGGAAGCCCCTCCGGGATTTATCAACTCAAACCATTCGGGAGATGTAT GCACTCACCCAAGGCCGAGTTCCCATAATTGGGGTTGGTGGTGTGAGCAGCGGGCAGGAC GTGCTGGAGAAGATCCGGGCAGGGGCCTCCCTGGTGCAGCTGTACACGGCCCTCACCTTC TGGGGGCCACCCGTTGTGGGCAAAGTCAAGCGGGAACTGGAGGCCCTTCTGAAAGAGCAG GGCTTTGGCGGAGTCACAGATGCCATTGGAGCAGATCATCGGAGGTGA Clone variation with respect to NM_001361.4 19 a=>c;1022 c=>t >OriGene 5' read for NM_001361 unedited
GACTCACTATAGGGCGGCCGCGAATTCGGCACCAGATGGCGTGGAGACACCTGCAAAAGC GGGCCCAGGATGCTGTGATCATCCTGGGGGGAGGAGGACTTCTCTTCGCCTCCTACCTGA TGGCCACGGGAGATGAGCGTTTCTATGCTGAACACCTGATGCCGACTCTGCAAGGGCTGC TGGACCCGGAGTCAACCCACAAACTGGCTGTTCGCTTCACCTTCCTGGGGCTCCTTTCAC GGGCCAAATTTCAAGACTCTGACATGCTGGAAGTGAGAGTTCTGGGCCATAAATTCCGAA ATCCAGTAAGAATTGCTGCAGGATTTGACAAGCATGGGGAAACCGTGGACCGACTTTATA AGATGGGCTTTGGTTTTGTTGAGATAGGAAGTGTGACTCCAAAACCTCAGGAAGGAAACC CTAGACCCAGAGTCTTCCGCCTTCCTGAAGACCAAGCTGTCATTAACAGGTATGGATTTA ACAGTCACGGGCTTTCAGTGGTGGAACACAAGTTACCGGCCAAACAGCAGAAGCAGGCCA AGCTCACAGAAGATGGACTGGCTCTGGGGGTCAACTTGGGGAAGAACAAGACCTCAGTGG ACGCCGCGGAGGACTACGCACAAAGGGTGCGCGTACTGGGCCCCCTGGGCGACTACCTGG TGGTGAATGTGTGCAGACCCAACACTGCCGGGCTGCCGAGCCTCTAAGGAAAGGCCGAGC TGCGCCCGCTGCTTG >OriGene 3' read for NM_001361 unedited
CCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAA ACAGCTATGACCTGTTGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTCCCAGGTTCTAAATGGCTTTATTTTTTCCATGAC TTTTTCCTCCTAATATTCAATGTCCTTGCAAGACTGATGGATCCTAGGGTTTGAATCCAC AAAGAATCAAAAATGCAGTTTATGGTCTGACCAAGGGACTGAAAAAAGCCTGCGGTTGAT CCTGGTGACCCCCCGATTGGCGTTTACAGTGCAGCCATGCCATGGCTGGGGGACATGGCT CAAGATGGAGTCCCTCCTCTCATGATCCAGCCACAAAGGCTTGCCTGAGTCCTTGGGAAG GTTCCAGATCAGGCTTCCCGTCAGACGCTGTCCTCACCTCCGATGATCTGCTCCAATGGC ATCTGTGACTCCGCCAAAGCCCTGCTCTTTCAGAAGGGCCTCCAGTTCCCGCTTGACTTT GCCCACAACGGGTGGCCCCCAAAAGGTGAGGGCCGTTACAGCTGCACAGGGAGGCCCTTG CCCGTTTTTTTTCCAACATTCTTGCCGTTGTTACCCACCACCCCAATTTTGGAACTCGCC TGGGTGATGCTACTCTCCAATGGTTGAGTTATAATCCCGAGGGCTTCCCTCACCCT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_001361 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001361.3, NP_001352.2 |
| RefSeq Size | 2080 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 1723 |
| UniProt ID | Q02127 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, Pyrimidine metabolism |
| Gene Summary | The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification
,null,
Nature chemical biology
,PubMed ID 27018887
[DHODH]
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| SC324054 | DHODH (untagged)-Human dihydroorotate dehydrogenase (DHODH), nuclear gene encoding mitochondrial protein |
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