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E2F5 (NM_001951) Human Untagged Clone
CAT＃: SC128153
E2F5 (untagged)-Human E2F transcription factor 5, p130-binding (E2F5), transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E2F-5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128153 sequence for NM_001951 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCANNNCCCGCGAGCTCGGGCCAGCAGGCGCCGGCAGGGCAGGGGCAGGGC CAGCGGCCGCCGCCGCAGCCTCCGCAGGCGCAAGCCCCGCAGCCGCCCCCGCCGCCGCAG CTCGGGGGCGCGGGGGGCGGCAGCAGCAGGCACGAGAAGAGCCTGGGGCTGCTCACTACC AAGTTCGTGTCGCTGCTGCAGGAGGCCAAGGACGGCGTTCTGGATCTCAAAGCGGCTGCT GATACTTTGGCTGTGAGGCAAAAAAGGAGAATTTATGATATCACCAATGTCTTAGAGGGA ATTGACTTGATTGAAAAAAAGTCAAAAAACAGTATCCAGTGGAAAGGTGTAGGTGCTGGC TGTAATACTAAAGAAGTCATAGATAGATTAAGATATCTTAAAGCTGAAATTGAAGATCTA GAACTGAAGGAAAGAGAACTTGATCAGCAGAAGTTGTGGCTACAGCAAAGCATCAAAAAT GTGATGGACGATTCCATTAATAATAGATTTTCCTATGTAACTCATGAAGACATCTGTAAT TGCTTTAATGGTGATACACTTTTGGCCATTCAGGCACCTTCTGGTACACAACTGGAGGTA CCCATTCCAGAAATGGGTCAGAATGGACAAAAGAAATACCAGATCAATCTAAAGAGTCAT TCAGGACCTATCCATGTGCTGCTTATAAATAAAGAGTCGAGTTCATCTAAGCCCGTGGTT TTTCCTGTTCCCCCACCTGATGACCTCACACAGCCTTCCTCCCAGTCCTTGACTCCAGTG ACTCCACAGAAATCCAGCATGGCAACTCAAAATCTGCCTGAGCAACATGTCTCTGAAAGA AGCCAGGCTCTGCAGCAGACATCAGCTACAGATATATCTTCAGCAGGATCTATTAGTGGA GATATCATTGATGAGTTAATGTCTTCTGACGTGTTTCCTCTCTTAAGGCTTTCTCCTACC CCGGCAGATGACTACAACTTTAATTTAGATGATAACGAAGGAGTTTGTGATCTGTTTGAT GTCCAGATACTAAATTATTAG Clone variation with respect to NM_001951.3 13 g=>n;14 a=>n;15 g=>n;132 c=>g >OriGene 5' read for NM_001951 unedited
TTCGGAATTTGTAATACGACTCTACTATAGGGCGGCCGCGAATCGGCACCAGGAAGCGGC CGCAGTGGAGCCGACCCGGCAGGTGGCCGCGGGCGGGGCCGGCGAGCGAAAGTGCGCGGG GGCCCGACCACCGCGGGGCCGGGACGCGATGGCGGCGGCAGAGCCCGCGAGCTCGGGCCA GCAGGCGCCGGCAGGGCAGGGGCAGGGCCAGCGGCCGCCGCCGCAGCCTCCGCAGGCGCA AGCCCCGCAGCCGCCCCCGCCGCCGCAGCTCGGGGGCGCGGGGGGCGGCAGCAGCAGGCA CGAGAAGAGCCTGGGGCTGCTCACTACCAAGTTCGTGTCGCTGCTGCAGGAGGCCAAGGA CGGCGTTCTGGATCTCAAAGCGGCTGCTGATACTTTGGCTGTGAGGCAAAAAAGGAGAAT TTATGATATCACCAATGTCTTAGAGGGAATTGACTTGATTGAAAAAAAGTCAAAAAACAG TATCCAGTGGAAAGGTGTANGTGCTGGCTGTAATACTAAAGAAGTCATAGATAGATTAAG ATATCTTAAAGCTGAAATTGAAGATCTAGAACTGAAGGAAAGAGAACTTGATCAGCAGAA GTTGTGGCTACAGCAAAGCATCAAAAATGTGATGGACGATTCCATTAATAATAGATTTTC CTATGTAACTCATGAAGACATCTGTNATTGCTNTAATGGTGATACACTTTTGGCCATTCA GGCACCTTCTGGTACACAACTGGNAGTACCCCATCCANAAATGGGTCAGAATGACAANAG AATACCAGATCAATCTAAGAATCATTCAGACCTATCCTGTGCTGCTTATAATAAAGATCC ATTCATCTAGCCCGTGGTTTTTCTGTCCCCCACTGATGACTACCAGNCTNCTCCAGTCCT GACTCCCGGACTCCCCAAATCCGCTGGCACTCAAACTGC >OriGene 3' read for NM_001951 unedited
NTAATTCAGNACGCGGCCGCATTCTANGATCAGTTTTTTTTTTTTTTTTTTGAATGTTTT ATACAATTTTATTTTTAAAAATCTTGTTAATGTACAGGCATTGGCACATTTTAAAAACAA ACTACATAAACAGATCTTTCCTATAACCTAGGAAAGTGGAATGTCAGAAGTCAACAAAAT GTGATAAACTTAAAGTGCTAAAACAGAAGGCACTTCACAAAATCTGTTCACTGAAACAGT TATATATCCTCGTTTACATCCTTCACTTTACAAGTGGCAGTGAACGTCTGTTTGGATAGA AGGACATACAGAAATACAGGCAGTTTAGTGGCAGTAAAAATATAAGACAAGTAATGAGTC CTTGGCCAACTTGTTTTTGATGACCTGTAGTGTCCTTTAACTTTCCTCCTTAAGAAGAAA AAACAGAAGGATTCACAATTAGAAGAAAAGTTGAAATCCAGAATATAGTTACTTTTGGGA GTGGGGACGTTTGTGGATATTAAATCACTTATCCCCTAAAGCAATCAGAGCCCTTTGTTT TTATGGTTGAAGTTTTGTGAAGTAAAGAAAGTATTAGGGAAGAACACTTCAGAATCAGTG AACTAAAAAAGGTGTTACATTCCTTATTTTAAATATTTAGGTTATTAAGAAGGCCAAAAT GTTACCCAGTTAGAGGTAGATAACAGTCCCAAGTTTTCCTGGAAATCTAAAATTTAATAT CCTGGACATCAAAAAAAATCACAAACTCCTTCGGTATCCTCCAAAATAAAGGTTGGAGTC CTCCGGCCCGGGTAAGGGGAAAGCCCTAAAAAAAGGAAACCCCCCGAAGAACATTACTCA CCAATGAAATCTCCACCAAAAAATCCGGGTGAAAAAATAACCTGGAACTGATGTCTGCTG CCGAACCTGGGTTTCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001951 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001951.2, NP_001942.1 |
| RefSeq Size | 1752 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 1875 |
| UniProt ID | Q15329 |
| Domains | E2F_TDP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Cell cycle, TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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NFkB is activated by multiple mechanisms in hairy cell leukemia
,Nagel, S;Ehrentraut, S;Meyer, C;Kaufmann, M;Drexler, HG;MacLeod, RA;,
Genes Chromosomes Cancer
,PubMed ID 25960206
[E2F5]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224285 | E2F5 (Myc-DDK-tagged)-Human E2F transcription factor 5, p130-binding (E2F5), transcript variant 1 |
CNY 5,488.00 |
|
| RC224285L3 | Lenti-ORF clone of E2F5 (Myc-DDK-tagged)-Human E2F transcription factor 5, p130-binding (E2F5), transcript variant 1 |
CNY 5,890.00 |
|
| RC224285L4 | Lenti-ORF clone of E2F5 (mGFP-tagged)-Human E2F transcription factor 5, p130-binding (E2F5), transcript variant 1 |
CNY 5,890.00 |
|
| RG224285 | E2F5 (tGFP-tagged) - Human E2F transcription factor 5, p130-binding (E2F5), transcript variant 1 |
CNY 7,088.00 |
