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OGA (NM_012215) Human Untagged Clone
CAT＃: SC128136
MGEA5 (untagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1
CNY 10,072.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MEA5; MGEA5; NCOAT |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC128136 sequence for NM_012215 edited (data generated by NextGen Sequencing)
ATGGTGCAGAAGGAGAGTCAAGCGACGTTGGAGGAGCGGGAGAGCGAGCTCAGCTCCAAC CCTGCCGCCTCTGCGGGGGCATCGCTGGAGCCGCCGGCAGCTCCGGCACCCGGAGAAGAC AACCCCGCCGGGGCTGGGGGAGCGGCGGTGGCCGGGGCTGCAGGAGGGGCTCGGCGGTTC CTCTGCGGTGTGGTGGAAGGATTTTATGGAAGACCTTGGGTTATGGAACAGAGAAAAGAA CTCTTTAGAAGGCTCCAGAAATGGGAATTAAATACATACTTGTATGCCCCAAAAGATGAC TACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAGGAAGCTGAGCAACTTATG ACTCTCATCTCTGCTGCACGAGAATATGAGATAGAGTTCATCTATGCGATCTCACCTGGA TTGGATATCACTTTTTCTAACCCCAAGGAAGTATCCACATTGAAACGTAAATTGGACCAG GTTTCTCAGTTTGGGTGCAGATCATTTGCTTTGCTTTTTGATGATATAGACCATAATATG TGTGCAGCAGACAAAGAGGTATTCAGTTCTTTTGCTCATGCCCAAGTCTCCATCACAAAT GAAATCTATCAGTACCTAGGAGAGCCAGAAACTTTCCTCTTCTGTCCCACAGAATACTGT GGCACTTTCTGTTATCCAAATGTGTCTCAGTCTCCATATTTAAGGACTGTGGGTGAAAAG CTTCTACCTGGAATTGAAGTGCTTTGGACAGGTCCCAAAGTTGTTTCTAAAGAAATTCCA GTAGAGTCCATCGAAGAGGTTTCTAAGATTATTAAGAGAGCTCCAGTAATCTGGGATAAC ATTCATGCTAATGATTATGATCAGAAGAGACTGTTTCTGGGCCCGTACAAAGGAAGATCC ACAGAACTCATCCCACGGTTAAAAGGAGTCCTCACTAATCCAAATTGTGAATTTGAAGCC AACTACGTTGTTATCCACACCCTTGCCACCTGGTACAAATCAAACATGAATGGAGTGAGA AAAGATGTAGTGATGACTGACAGTGAAGATAGTACTGTGTCCATCCAGATAAAATTAGAA AATGAAGGCAGTGATGAAGATATTGAAACTGATGTACTCTATAGTCCACAGATGGCTCTA AAGCTAGCATTAACAGAATGGTTGCAAGAGTTTGGTGTGCCTCATCAATACAGCAGTAGG CAAGTTGCACACAGTGGAGCTAAAGCAAGTGTAGTTGATGGGACTCCTTTAGTTGCAGCA CCCTCTTTAAATGCCACAACCGTAGTAACAACAGTTTATCAGGAGCCCATTATGAGCCAG GGAGCAGCCTTGAGTGGTGAGCCTACTACTCTGACCAAGGAAGAAGAAAAGAAACAGCCT GATGAAGAACCCATGGACATGGTGGTGGAAAAACAAGAAGAAACGGACCACAAGAATGAC AATCAAATACTGAGTGAAATTGTTGAAGCGAAAATGGCAGAGGAATTGAAACCAATGGAC ACTGATAAAGAGAGCATAGCTGAATCAAAATCCCCAGAGATGTCCATGCAAGAAGATTGT ATTAGTGACATTGCCCCCATGCAAACTGATGAACAGACAAACAAGGAGCAGTTTGTGCCA GGTCCAAATGAAAAGCCTTTGTACACTGCGGAACCAGTGACCCTGGAGGATTTGCAGTTA CTTGCTGATCTATTCTACCTTCCTTACGAGCATGGACCCAAAGGAGCACAGATGTTACGG GAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTGTCAATTGCAAAGGAAAAGAC TCTGAAAAAATTGAAGAATGGCGGTCACGAGCAGCCAAGTTTGAAGAGATGTGTGGACTA GTGATGGGAATGTTCACTCGGCTCTCCAATTGTGCCAACAGGACAATTCTTTATGACATG TACTCCTATGTTTGGGATATCAAGAGTATAATGTCTATGGTGAAGTCTTTTGTACAGTGG TTAGGGTGTCGTAGTCATTCTTCAGCACAATTCTTAATTGGAGACCAAGAACCCTGGGCC TTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGGGCAAATGAT CTCTTTTTTCAGCCACCTCCACTGACTCCTACCTCCAAAGTTTATACTATCAGACCTTAT TTTCCTAAGGATGAGGCATCCGTGTACAAGATTTGCAGAGAAATGTATGACGATGGAGTG GGTTTACCCTTTCAAAGTCAGCCTGATCTTATTGGAGACAAGTTAGTAGGAGGGCTGCTT TCCCTCAGCCTGGATTACTGCTTTGTCCTAGAAGATGAAGATGGCATATGTGGTTATGCC TTGGGCACTGTAGATGTGACCCCCTTTATTAAAAAATGTAAAATTTCCTGGATCCCCTTC ATGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTGAGGCTGAGAAAATA ATGTTGAGTTTCCATGAAGAACAGGAAGTACTGCCAGAAACTTTCCTTGCTAATTTCCCT TCTCTGATAAAGATGGACATTCACAAAAAAGTAACTGACCCAAGTGTGGCCAAAAGCATG ATGGCTTGCCTCCTGTCTTCACTGAAGGCTAATGGCTCCCGGGGAGCTTTCTGTGAAGTG AGACCAGATGATAAAAGAATTCTGGAATTTTACAGCAAGTTAGGATGTTTTGAAATTGCA AAAATGGAAGGATTTCCAAAGGATGTGGTTATACTTGGTCGGAGCCTGTGA Clone variation with respect to NM_012215.3 971 c=>t |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012215 |
| Insert Size | 4730 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012215.1, NP_036347.1 |
| RefSeq Size | 5147 bp |
| RefSeq ORF | 2751 bp |
| Locus ID | 10724 |
| UniProt ID | O60502 |
| Gene Summary | The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222411 | MGEA5 (Myc-DDK-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 |
CNY 10,064.00 |
|
| RC222411L1 | Lenti-ORF clone of MGEA5 (Myc-DDK-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 |
CNY 12,464.00 |
|
| RC222411L2 | Lenti-ORF clone of MGEA5 (mGFP-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 |
CNY 11,020.00 |
|
| RC222411L3 | Lenti-ORF clone of MGEA5 (Myc-DDK-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 |
CNY 11,020.00 |
|
| RC222411L4 | Lenti-ORF clone of MGEA5 (mGFP-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 |
CNY 11,020.00 |
|
| RG222411 | MGEA5 (tGFP-tagged) - Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1 |
CNY 11,664.00 |
