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Spastin (SPAST) (NM_014946) Human Untagged Clone
CAT＃: SC128070
SPAST (untagged)-Human spastin (SPAST), transcript variant 1
CNY 6,888.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADPSP; FSP2; SPG4 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_014946, the custom clone sequence may differ by one or more nucleotides
ATGAATTCTCCGGGTGGACGAGGGAAGAAGAAAGGCTCCGGCGGCGCCAGCAACCCGGTGCCTCCCAGGC CTCCGCCCCCTTGCCTGGCCCCCGCCCCTCCCGCCGCCGGGCCGGCCCCTCCGCCCGAGTCGCCGCATAA GCGGAACCTGTACTATTTCTCCTACCCGCTGTTTGTAGGCTTCGCGCTGCTGCGTTTGGTCGCCTTCCAC CTGGGGCTCCTCTTCGTGTGGCTCTGCCAGCGCTTCTCCCGCGCCCTCATGGCAGCCAAGAGGAGCTCCG GGGCCGCGCCAGCACCTGCCTCGGCCTCGGCCCCGGCGCCGGTGCCGGGCGGCGAGGCCGAGCGCGTCCG AGTCTTCCACAAACAGGCCTTCGAGTACATCTCCATTGCCCTGCGCATCGATGAGGATGAGAAAGCAGGA CAGAAGGAGCAAGCTGTGGAATGGTATAAGAAAGGTATTGAAGAACTGGAAAAAGGAATAGCTGTTATAG TTACAGGACAAGGTGAACAGTGTGAAAGAGCTAGACGCCTTCAAGCTAAAATGATGACTAATTTGGTTAT GGCCAAGGACCGCTTACAACTTCTAGAGAAGATGCAACCAGTTTTGCCATTTTCCAAGTCACAAACGGAC GTCTATAATGACAGTACTAACTTGGCATGCCGCAATGGACATCTCCAGTCAGAAAGTGGAGCTGTTCCAA AAAGAAAAGACCCCTTAACACACACTAGTAATTCACTGCCTCGTTCAAAAACAGTTATGAAAACTGGATC TGCAGGCCTTTCAGGCCACCATAGAGCACCTAGTTACAGTGGTTTATCCATGGTTTCTGGAGTGAAACAG GGATCTGGTCCTGCTCCTACCACTCATAAGGGTACTCCGAAAACAAATAGGACAAATAAACCTTCTACCC CTACAACTGCTACTCGTAAGAAAAAAGACTTGAAGAATTTTAGGAATGTGGACAGCAACCTTGCTAACCT TATAATGAATGAAATTGTGGACAATGGAACAGCTGTTAAATTTGATGATATAGCTGGTCAAGACTTGGCA AAACAAGCATTGCAAGAAATTGTTATTCTTCCTTCTCTGAGGCCTGAGTTGTTCACAGGGCTTAGAGCTC CTGCCAGAGGGCTGTTACTCTTTGGTCCACCTGGGAATGGGAAGACAATGCTGGCTAAAGCAGTAGCTGC AGAATCGAATGCAACCTTCTTTAATATAAGTGCTGCAAGTTTAACTTCAAAATACGTGGGAGAAGGAGAG AAATTGGTGAGGGCTCTTTTTGCTGTGGCTCGAGAACTTCAACCTTCTATAATTTTTATAGATGAAGTTG ATAGCCTTTTGTGTGAAAGAAGAGAAGGGGAGCACGATGCTAGTAGACGCCTAAAAACTGAATTTCTAAT AGAATTTGATGGTGTACAGTCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAATAGGCCACAA GAGCTTGATGAGGCTGTTCTCAGGCGTTTCATCAAACGGGTATATGTGTCTTTACCAAATGAGGAGACAA GACTACTTTTGCTTAAAAATCTGTTATGTAAACAAGGAAGTCCATTGACCCAAAAAGAACTAGCACAACT TGCTAGAATGACTGATGGATACTCAGGAAGTGACCTAACAGCTTTGGCAAAAGATGCAGCACTGGGTCCT ATCCGAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTG ACTTCACTGAATCCTTGAAAAAAATAAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTTG GAACAAGGACTTTGGAGATACCACTGTTTAA >OriGene 5' read for NM_014946 unedited
NNNTTTTNNGGGGGGNNNNNNNNNNNNCCCNNNNNATTTAAATACACCCGCCCGTTGNCG CAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGACA CTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCAGCG ACAGGAAGGGAGGGGCCCGAGCCACCGACTGCAGGAGGAGAAGGGGTTGTGCTCCTGGCC GAGGAAGGAGAAAGGGGCGGGGCCGGCGGGCAGCGTGCGGCAGTGCGGAGCTCCTGAGAC CGGCGGGCACACGGGGGTCTGTGGCCCCCGCCGTAGCAGTGGCTGCCGCCGTCGCTTGGT TCCCGTCGGTCTGCGGGAGGCGGGTTATGGCGGCGGCGGCAGTGAGAGCTGTGAATGAAT TCTCCGGGTGGACGAGGGAAGAAGAAAGGCTCCGGCGGCGCCAGCAACCCGGTGCCTCCC AGGCCTCCGCCCCCTTGCCTGGCCCCCGCCCCTCCCGCCGCCGGGCCGGCCCCTCCGCCC GAGTCGCAGCATAAGCGGAACCTGTACTATTTCTCCTACCCGCTGTTTGTAGGCTTCGCG CTGCTGCGTTTGGTCGCCTTCCACCTGGGGCTCCTCTTCGTGTGGCTCTGCCAGCGCTTC TCCCGCGCCCTCATGGCAGCCAAGAGGAGCTCCGGNGCCGCGCCAGCACCTGCCTCGGCC TCGGCCCGGGCGCCGGTGCCGGGCGGCGAGGCCGAGCGCGTCCCGAGTCTTCCACAAACA GGCCTTCGAGTACATCTCCATTGCCCTGCGCATCGATGGAAGATGAGCACAGCAGGACAG GAAGAGCAAGCTGGTGGAATGTTATAAGAAAGGTTTTTCGAGAACTGGGAAAAGGCAAAA GCCTGTTTTAACTTCCGGAACCAGGCGGAA >OriGene 3' read for NM_014946 unedited
GGGAGGCGTTCACTGCATTCAACTTCCACAGCCAGGNAGAGGANTGAGGAGGGGTCACAG GGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGA GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGTTTATCATTTATTTCCAATACATT AGGATAAAAACCCACTTTATGCAACTGTACCCTTTAATTCATTTCTTCTGAAGAAAAGTA CAAAAGACCAGACTAAAATTGAGACAAGCATTTAAAGGAAAATCCTTTGACTCAAAGTCC AAAACCGAACTGTAAATGGTTCATTTTACCAAAAGAATTAGTTTTTGTACAAAATATAAA GAATTCTACACTATAAACAAAGACGGCTGTATATGTAATTAAATTAGAATTCTAGTATCT GCTTTTCCTCTAATTCTTCTGGAGTACAAACATATTTGCAAGTCAGAAATCTAATATTCT CAATGGACAGACTGTCTTAGAATAAGCTTTTACCCTTAAGATATCTGGAAAGATAGTACT CATTCTTTCCCTTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014946 |
| Insert Size | 5200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014946.3, NP_055761.2 |
| RefSeq Size | 5221 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 6683 |
| UniProt ID | Q9UBP0 |
| Domains | AAA, AAA, MIT |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The use of alternative translational initiation sites in this gene results in a single transcript variant that can produce isoforms that differ in the length of their N-terminus and which thereby differ in the efficiency of their export from the nucleus to the cytoplasm. In addition, alternative splicing results in multiple transcript variants that encode isoforms that differ in other protein regions as well. One isoform of this gene has been shown to be a microtubule-severing enzyme that regulates microtubule abundance, mobility, and plus-end distribution. Mutations in this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, May 2018] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as M1). It also encodes a shorter isoform (M87) from an alternate, in-frame, downstream translation initiation site. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220458 | SPAST (Myc-DDK-tagged)-Human spastin (SPAST), transcript variant 1 |
CNY 6,880.00 |
|
| RC220458L1 | Lenti ORF clone of Human spastin (SPAST), transcript variant 1, Myc-DDK-tagged |
CNY 9,280.00 |
|
| RC220458L2 | Lenti ORF clone of Human spastin (SPAST), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC220458L3 | Lenti ORF clone of Human spastin (SPAST), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC220458L4 | Lenti ORF clone of Human spastin (SPAST), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG220458 | SPAST (tGFP-tagged) - Human spastin (SPAST), transcript variant 1 |
CNY 8,480.00 |
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