RASSF8 (AY665468) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C12orf2; HOJ1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AY665468, the custom clone sequence may differ by one or more nucleotides ATGGAACTTAAAGTATGGGTGGATGGAGTTCAGAGGATTGTTTGTGGAGTCACTGAAGTCACAACTTGCC AGGAGGTTGTCATAGCCTTAGCTCAAGCAATAGGTCGAACTGGAAGGTACACCCTTATAGAGAAATGGAG AGATACTGAAAGACACTTAGCACCTCATGAAAATCCTATCATATCCTTAAACAAATGGGGGCAGTATGCT AGTGATGTGCAGCTCATTCTACGACGAACTGGGCCGTCTCTCAGTGAGCGACCCACTTCAGACAGTGTGG CTCGAATTCCTGAAAGAACTTTATACAGGCAGAGTCTGCCCCCCTTAGCTAAACTGAGGCCTCAGATTGA CAAATCAATCAAAAGGAGGGAACCGAAAAGGAAATCACTGACATTTACAGGAGGTGCCAAAGGATTAATG GACATTTTTGGAAAAGGTAAAGAAACTGAGTTTAAGCAAAAGGTGCTGAATAACTGCAAAACAACAGCAG ATGAGTTGAAGAAGCTAATCCGTCTGCAGACAGAGAAGCTTCAATCCATTGAGAAACAGCTGGAATCTAA TGAAATAGAAATAAGATTTTGGGAGCAAAAGTATAATTCCAACCTTGAAGAGGAAATTGTCCGTCTAGAG CAAAAGATCAAAAGAAACGATGTAGAAATTGAGGAGGAAGAATTCTGGGAAAATGAATTACAGATTGAAC AGGAAAATGAAAAACAGCTGAAGGATCAACTTCAAGAAATAAGACAGAAAATAACAGAATGTGAAAACAA ATTAAAGGACTATTTGGCACAGATCCGGACTATGGAAAGTGGTCTTGAAGCAGAAAAATTGCAACGGGAA GTTCAAGAGGCACAGGTCAATGAGGAAGAGGTTAAAGGAAAGATCGGTAAGGTCAAAGGGGAGATTGACA TTCAAGGCCAGCAGAGTCTGAGGTTGGAAAATGGCATCAAAGCTGTGGAAAGATCTCTTGGACAAGCCAC CAAACGCTTACAGGACAAAGAACAGGAACTGGAGCAGTTGACTAAGGAGTTGCGGCAAGTCAATCTCCAG CAGTTCATCCAGCAGACAGGGACAAAAGTTACCGTTTTGCCAGCGGAGCCCATTGAAATAGAGGCCTCAC ATGCAGACATTGAAAGGGAGGCACCATTCCAGTCTGGGTCCCTGAAGCGACCTGGTTCATCTCGGCAGCT CCCCAGTAATCTCCGCATTCTGCAGAATCCTATCTCATCTGGTTTTAATCCTGAAGGCATATATGTATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AY665468 unedited GGACATGACCTAACACCCTGATGACCACTCTCAGGGACCTTGAGTGACTGGCCGGTGCAC CATGGAACTTAAAGTATGGGTGGATGGAGTTCAAAGGATTGTTTGTGGAGTCACTGAAGT CACAACTTGCCAGGAGGTTGTCATAGCCTTAGCTCAAGCAATAGGTCGAACTGGAAGGTA CACCCTTATAGAGAAATGGAGAGATACTGAAAGACACTTAGCACCTCATGAAAATCCTAT CATATCCTTAAACAAATGGGGGCAGTATGCTAGTGATGTGCAGCTCATTCTACGACGAAC CGGGCCGTCTCTCAGTGAGCGACCCACTTCAGACAGTGTGGCTCGAATTCCTGAAAGAAC TTTATACAGGCAGAGTCTGCCCCCCTTATCTAAACTGAGGCCTCAGATTGACGAATCAAT CAAAAGGAGGGAACCGAAAAGGAAATCACTGACATTTACAGGAGGTGCCAAAGGATTAAT GGACATTTTTGGAAAAGGTAAAAAACTGAGTTTAAGCAAAAAGTGCTGAATAACNTGCAA ACACAGCAGATGAGTTGAAGAGCTTATCCGTCTGCAGAAGAGAAGCTTCTATCCATTGAG AAACAGCTGGNATCTAATAAAAAGAAATAAATTTTTGGAGCAAAAGATAATTCCCACCTG ACAGGAATATGGNCGTCTGACCAAAGTCAAAGAAAAATGTTAAATTGAGGGGGGAAAATT TGGGAATAGGATTACCCTTGACCGGAANAGAAAAACCCGAGGGCCCCTCACAAAATAACA AAAAAAGAATGGAAAAAAAAAAGCTTTGGTCAATCGCTTTT 3' Read Nucleotide Sequence (showhide) >Forward primer walk for AY665468 unedited CATCATATACACATTCATCCGCANACAGGNCAAAGTTACCGTTTTGCCGCGGAGCCCATT GAATAGAGGCCTCACATGCAGACATTGAAAGGGAGGCACCATTCCAGTCTGGGTCCCTGA AGCGACCTGGTTCATCTCGGCAGCTCCCCAGTAATCTCCGCATTCTGCAGAATCCTATCT CATCTGGTTTTAATCCTGAAGGCATATATGTATGACATTATCTGTCTTTAGGGAGGAGAC CCAACAGAGGTACCAAGGACAGTAAACTTCCTTTTTGATTTGTGCCAATGATGAACAGAG GATCTATTCCACAAGACGCTGTATGTTTTTTCTCTCCTAAATTGCATACCACTTGGAGCC ATACCCTGTGCACTGATGCTAAAGAACAAAGAAACTGTGTTTTCACACATCAACAGTGTT GATATTTTTGTCCAGCAGCTATAATGCAAAGCCTTCGTTTTTATTTGTAGCATTTTGAGA GCTTTAAGAAAGTACTATATAGTGTTGTTACATAAATAATCCCGGGCTTAAGTAAGAGTG AAAAGAGAATTGGGGACTGGAGTTGCTACGATTTACTGCCGGGAACCGAAGTGCGAACCG TGAAAGAAACAAAAGTGTATAAAGGGGTGAGCGTTAGCCGAGAATAAGAGAGATATAGGG GGCATTCCTGTTGAGCAGACTGAGGCAAAAAGGGAAACGAGTAACTAAACCCGATTAGGA AAAGCTAATGAGGAGCGAACTGTAAATACACAGACCCGAGCAATCGACAGACTTATAAAG GGGGAGTACGATACGACACTTAAAGCTGGCCCGACAAAGGCGCACAAGGCGAGAAATGAA CAGAGGGTGAATT
Restriction Sites	NotI-NotI
ACCN	AY665468
OTI Disclaimer	The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
OTI Annotation	This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AY665468.1, AAV54603.1
RefSeq Size	5472 bp
RefSeq ORF	5472 bp
Locus ID	11228
Gene Summary	This gene encodes a member of the Ras-assocation domain family (RASSF) of tumor suppressor proteins. This gene is essential for maintaining adherens junction function in epithelial cells and has a role in epithelial cell migration. It is a lung tumor suppressor gene candidate. A chromosomal translocation t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the FBLN1 gene is found in a complex type of synpolydactyly. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

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