NEK8 (NM_178170) Human Untagged Clone
CAT#: SC127876
NEK8 (untagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8)
CNY 5,080.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | JCK; NEK12A; NPHP9; RHPD2 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_178170, the custom clone sequence may differ by one or more nucleotides
ATGGAGAAGTACGAGCGGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGCGAA AGGCTGACCAGAAGCTGGTGATCATCAAGCAGATTCCAGTGGAACAGATGACCAAGGAAGAGCGGCAGGC AGCCCAGAATGAGTGCCAGGTCCTCAAGCTGCTCAACCACCCCAATGTCATTGAGTACTACGAGAACTTC CTGGAAGACAAAGCCCTTATGATCGCCATGGAATATGCACCAGGCGGCACTCTGGCTGAGTTCATCCAAA AGCGCTGTAATTCCCTGCTGGAGGAGGAGACCATCCTGCACTTCTTCGTGCAGATCCTGCTTGCACTGCA TCATGTGCACACCCACCTCATCCTGCACCGAGACCTCAAGACCCAGAACATCCTGCTTGACAAACACCGC ATGGTCGTCAAGATCGGTGATTTCGGCATCTCCAAGATCCTTAGCAGCAAGAGCAAGGCCTACACGGTGG TGGGTACCCCATGCTATATCTCCCCTGAGCTGTGTGAGGGCAAGCCCTACAACCAGAAGAGTGACATCTG GGCCCTGGGCTGTGTCCTCTACGAGCTGGCCAGCCTCAAGAGGGCTTTCGAGGCTGCGAACTTGCCAGCA CTGGTGCTGAAGATCATGAGTGGCACCTTTGCACCTATCTCTGACCGGTACAGCCCTGAGCTTCGCCAGC TGGTCCTGAGTCTACTCAGCCTGGAGCCTGCCCAGCGGCCACCACTCAGCCACATCATGGCACAGCCCCT CTGCATCCGTGCCCTCCTCAACCTCCACACCGACGTGGGCAGTGTCCGCATGCGGAGGGCAGAGAAGTCC GTGGCCCCCAGCAACACAGGGAGCAGGACCACCAGTGTCCGCTGCAGAGGTATCCCCCGGGGACCTGTGA GGCCAGCCATCCCACCACCACTGTCGTCAGTGTATGCCTGGGGTGGTGGGCTGGGCACCCCCCTGCGGCT GCCAATGCTCAACACAGAGGTGGTCCAGGTGGCAGCTGGGCGCACGCAGAAAGCCGGCGTCACGCGCTCT GGGCGTCTCATCCTGTGGGAGGCCCCACCCCTAGGTGCAGGCGGAGGCAGTCTCCTTCCTGGGGCAGTGG AGCAGCCACAGCCCCAGTTCATCTCGCGTTTCCTGGAGGGCCAGTCGGGTGTGACCATCAAGCACGTGGC CTGTGGGGACTTCTTCACTGCCTGCCTGACTGACAGAGGCATCATCATGACATTCGGCAGCGGCAGCAAT GGGTGCCTAGGCCATGGCAGCCTCACTGACATCAGCCAGCCCACCATTGTGGAGGCTTTGCTGGGCTATG AAATGGTGCAGGTGGCCTGTGGGGCCTCTCACGTGCTGGCCCTGTCCACTGAGCGAGAACTATTTGCCTG GGGCCGTGGAGACAGCGGCAGACTGGGGCTAGGCACCAGGGAGTCCCACAGCTGCCCCCAGCAGGTGCCC ATGCCCCCAGGACAGGAAGCTCAGCGAGTTGTATGTGGTATCGATTCCTCCATGATCCTCACTGTGCCTG GCCAAGCCCTAGCCTGTGGGAGCAACAGGTTCAACAAGCTGGGCCTGGACCACCTCTCCCTGGGGGAGGA GCCTGTCCCCCACCAGCAAGTGGAGGAGGCCCTGAGCTTCACACTACTAGGCTCTGCACCCCTGGACCAG GAGCCTCTGCTGAGTATAGACCTGGGCACTGCTCACTCAGCTGCTGTGACTGCCTCGGGTGATTGCTACA CTTTTGGCAGCAATCAGCACGGACAGTTGGGCACCAATACTCGCCGAGGCAGTCGGGCACCCTGTAAGGT CCAAGGCCTTGAGGGCATCAAGATGGCAATGGTAGCCTGTGGGGATGCCTTCACTGTAGCTATTGGGGCA GAGAGCGAAGTGTACTCTTGGGGCAAAGGGGCGCGAGGTCGATTGGGAAGGAGGGATGAGGATGCCGGAC TCCCTCGGCCAGTGCAGTTGGATGAGACACACCCTTACACGGTGACTTCCGTGTCCTGTTGCCATGGAAA CACCCTCCTGGCTGTTCGATCGGTCACAGATGAGCCGGTCCCCCCCTGA >OriGene 5' read for NM_178170 unedited
AGACTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGATGGAGA AGTACGAGCGGATCCGAGTGGTGGGGAGAGGTGCCTTCGGGATTGTGCACCTGTGCCTGC GAAAGGCTGACCAGAAGCTGGTGATCATCAAGCAGATTCCAGTGGAACAGATGACCAAGG AAGAGCGGCAGGCAGCCCAGAATGAGTGCCAGGTCCTCAAGCTGCTCAACCACCCCAATG TCATTGAGTACTACGAGAACTTCCTGGAAGACAAAGCCCTTATGATCGCCATGGAATATG CACCAGGCGGCACTCTGGCTGAGTTCATCCAAAAGCGCTGTAATTCCCTGCTGGAGGAGG AGACCATCCTGCACTTCTTCGTGCAGATCCTGCTTGCACTGCATCATGTGCACACCCACC TCATCCTGCACCGAGACCTCAAGACCCAGAACATCCTGCTTGACAAACACCGCATGGTCG TCAAGATCGGTGATTTCGGCATCTCCAAGATCCTTAGCAGCAAGAGCAAGGCCTACACGG TGGTGGGTACCCCATGCTATATCTCCCCTGAGCTGTGTGAGGGCAAGCCCTACAACCAGA AGAGTGACATCTGGGCCCTGGGCTGTGTCCTCTACGAGCTGGCCAGCCTCAAGAGGGCTT TCGAGGCTGCGAACTTGCCAGCACTGGTGCTGAAGATCATGAGTGGCANCTTTNGCACTA TCTCTGACGGTACAGCCNTGAGCTTCNCCGCTTGTCCTGATCTACTCACCTGGAGCTGGC CAGGGCACCATAACACATATGGAAAGCCCTTGATCGTGCCTCTAACTCCACNCATGGATG TCCTGCGAGAAAATCCGG |
Restriction Sites | Please inquire |
ACCN | NM_178170 |
Insert Size | 2700 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_178170.2, NP_835464.1 |
RefSeq Size | 2858 bp |
RefSeq ORF | 2079 bp |
Locus ID | 284086 |
UniProt ID | Q86SG6 |
Protein Families | Druggable Genome, Protein Kinase |
Gene Summary | This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Transcribed pseudogene PPM1K generates endogenous siRNA to suppress oncogenic cell growth in hepatocellular carcinoma
,Wen-Ling Chan, Chung-Yee Yuo, Wen-Kuang Yang, Shih-Ya Hung, Ya-Sian Chang, Chien-Chih Chiu, Kun-Tu Yeh, Hsien-Da Huang, and Jan-Gowth Chang,
Nucleic Acids Res., Feb 2013; 10.1093/nar/gkt047.
[NEK8]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216021 | NEK8 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 5,072.00 |
|
RC216021L1 | Lenti-ORF clone of NEK8 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 7,472.00 |
|
RC216021L2 | Lenti-ORF clone of NEK8 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 9,220.00 |
|
RC216021L3 | Lenti-ORF clone of NEK8 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 9,220.00 |
|
RC216021L4 | Lenti-ORF clone of NEK8 (mGFP-tagged)-Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 9,220.00 |
|
RG216021 | NEK8 (tGFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 8,080.00 |
|
SC323448 | NEK8 (untagged)-Kinase deficient mutant (K33M) of Human NIMA (never in mitosis gene a)- related kinase 8 (NEK8) |
CNY 5,080.00 |