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Aromatase (CYP19A1) (NM_000103) Human Untagged Clone
CAT＃: SC127848
CYP19A1 (untagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1
CNY 3,752.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127848 sequence for NM_000103 edited (data generated by NextGen Sequencing)
ATGGTTTTGGAAATGCTGAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCC ATGCCTGCTGCCACCATGCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAAT TATGAGGGCACATCCTCAATACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATC TCCCACGGCAGATTCCTGTGGATGGGGATCGGCAGTGCCTGCAACTACTACAACCGGGTA TATGGAGAATTCATGCGAGTCTGGATCTCTGGAGAGGAAACACTCATTATCAGCAAGTCC TCAAGTATGTTCCACATAATGAAGCACAATCATTACAGCTCTCGATTCGGCAGCAAACTT GGGCTGCAGTGCATCGGTATGCATGAGAAAGGCATCATATTTAACAACAATCCAGAGCTC TGGAAAACAACTCGACCCTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATG GTCACAGTCTGTGCTGAATCCCTCAAAACACATCTGGACAGGTTGGAGGAGGTGACCAAT GAATCGGGCTATGTGGACGTGTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTAAC ACGCTCTTCTTGAGGATCCCTTTGGACGAAAGTGCTATCGTGGTTAAAATCCAAGGTTAT TTTGATGCATGGCAAGCTCTCCTCATCAAACCAGACATCTTCTTTAAGATTTCTTGGCTA TACAAAAAGTATGAGAAGTCTGTCAAGGATTTGAAAGATGCCATAGAAGTTCTGATAGCA GAAAAAAGACGCAGGATTTCCACAGAAGAGAAACTGGAAGAATGTATGGACTTTGCCACT GAGTTGATTTTAGCAGAGAAACGTGGTGACCTGACAAGAGAGAATGTGAACCAGTGCATA TTGGAAATGCTGATCGCAGCTCCTGACACCATGTCTGTCTCTTTGTTCTTCATGCTATTT CTCATTGCAAAGCACCCTAATGTTGAAGAGGCAATAATAAAGGAAATCCAGACTGTTATT GGTGAGAGAGACATAAAGATTGATGATATACAAAAATTAAAAGTGATGGAAAACTTCATT TATGAGAGCATGCGGTACCAGCCTGTCGTGGACTTGGTCATGCGCAAAGCCTTAGAAGAT GATGTAATCGATGGCTACCCAGTGAAAAAGGGGACAAACATTATCCTGAATATTGGAAGG ATGCACAGACTCGAGTTTTTCCCCAAACCCAATGAATTTACTCTTGAAAATTTTGCAAAG AATGTTCCTTATAGGTACTTTCAGCCATTTGGCTTTGGGCCCCGTGGCTGTGCAGGAAAG TACATCGCCATGGTGATGATGAAAGCCATCCTCGTTACACTTCTGAGACGATTCCACGTG AAGACATTGCAAGGACAGTGTGTTGAGAGCATACAGAAGATACACGACTTGTCCTTGCAC CCAGATGAGACTAAAAACATGCTGGAAATGATCTTTACCCCAAGAAACTCAGACAGGTGT CTGGAACACTAG Clone variation with respect to NM_000103.3 >OriGene 5' read for NM_000103 unedited
AGTTCACAATTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACCAGGGCAAACAGG AAGGTGAAGAAGAACTTATCCTATCAGGACGGAAGGTCCTGTGCTCGGGATCTTCCAGAC GTCGCGACTCTAAATTGCCCCCTCTGAGGTCAAGGAACACAAGATGGTTTTGGAAATGCT GAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCCATGCCTGCTGCCACCAT GCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAATTATGAGGGCACATCCTC AATACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATCTCCCACGGCAGATTCCT GTGGATGGGGATCGGCAGTGCCTGCAACTACTACAACCGGGTATATGGAGAATTCATGCG AGTCTGGATCTCTGGAGAGGAAACACTCATTATCAGCAAGTCCTCAAGTATGTTCCACAT AATGAAGCACAATCATTACAGCTCTCGATTCGGCAGCAAACTTGGGCTGCAGTGCATCGG TATGCATGAGATAGGCATCATATTTAACAACAATCCAGAGCTCTGGAAAACAACTCGACC CTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATGGTCACAGTCTGTGCTGA ATCCCTCANAACACATCTGGACAGGTTGGAGGATGTGACCAATGAATCGGGCTTGTGGNA CGTGTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTACACGCTCTTCTTGANGATC CCTTTGGACGAAGTGCTATCGTGGNTAAATNCAAGGTATTNTGATGCATGGCAGCTCTCT CATCAACAGAATCTCTTTAGATTTCTGCTTACAAAGTTGANAAGCGTCAAGATTGAAGAT CCCTAAAGTCTGATACGAAAAGAGCAGATTNCCGAAAGACTGGANATGATGACTTGNCCT GGATGTTTACNAAAACTGTGACGA >OriGene 3' read for NM_000103 unedited
CGTTTCTTTTCCCCCCCCCCCCAATGCTGGGGAGAAGAGCCAATTGTGAGTGTTTGAATG ACATTCAGTAGAGATAGTTATATTGGCTATATTACACAAGTAAAGTGGTGTTTGGAAAGT TCCTACATTCATTTGATTTCCTTTAGAAAGCGAATTCCAAGGTGTGCAATTTATCCATGA CCACTTACATTGATTCATGGAACCAAAATATGTTAGATTCCCTCAGGTAACCGGTTAAAA TTTTGAGGTTGGAGTTAAAAAAATACTTCAGGGGGGTTTTACTTTTGGACCACCGTTTTT TTATTGGAGTGGGGGCCCCCTAGGATCTTTGGGGGCAGGGCGAAAAAAAAAGGGGGAAAA TATTTTCCGGGGGGAAAGAATAATAGGGGGTTAAAAGAGAGAGGCCCCCTCTTAAGAGAG TGGACACCCCCCTGGGGGGGGGGGATTTTTTTTTTCCCCAACAAACCAATCCCCTTGTTA TAAAGAAAACACCAAAATGGGGGCAGAAAACACGCCGGTTCCTTCTGGTGTGGCGCCCCC CCTCTTATTTTGGCACAACACAAAAAAGGGGGAGCACACCCCCCCCAGAGCTTCTTTTTT TTATTTATCTTAAAGAGGGCGCGCGGCGCGAAGATGTCTTTCCTTTCCTAAAAAAGAAAG AGGGCCGGCGCCAATATTAATCCCCCCCCCCCCTCTCTTAAATATATTTTTTGCCCCCGT GGGGGATAATATTAAAATTTGTTTCTCCCCCCATCCACACCAGCCACACCACACAAAATA TTGTCTCANTGTCGCCTCGGGCCGTGCGGCGCGTCTACGACCAACACCGGCGGCGGAGAA AACACTTCTGCCTCAATAAATTTCTCCCCCCCCCCCCCACATTTATTGTTCCACAAAACA AAAAGGTTTGTTTTCTTTTTTTTTAAATCGGCGCCAAGACCCGAACGTNTTTTACCACCT CCTAAACCACCATAAAGATCTTTTTTCGTTCCCCCCCCCCCCCCACACAAAAAAAAATTG TTCCTTCTCTCTCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000103 |
| Insert Size | 1512 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000103.2, NP_000094.2 |
| RefSeq Size | 3007 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 1588 |
| UniProt ID | P11511 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450 |
| Protein Pathways | Androgen and estrogen metabolism, Metabolic pathways |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1) represents the use of an alternate promoter and contains an alternate 5' exon (I.1), compared to variant 4. All variants encode the same protein. The 5' end of this transcript contains long terminal repeat (LTR) sequence, initiates from an LTR promoter and results in placenta-specific expression. (PMID: 19577618). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Increased adipose tissue aromatase activity improves insulin sensitivity and reduces adipose tissue inflammation in male mice
,Ohlsson, C;Hammarstedt, A;Vandenput, L;Saarinen, N;Ryberg, H;Windahl, S;Farman, HH;Janson, JO;Movérare-Skrtic, S;Smith, U;Zhang, FP;Poutanen, M;Hedjazifar, S;Sjogren, K;,
Am. J. Physiol. Endocrinol. Metab.
,PubMed ID 28655716
[CYP19A1]
|
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Effect of sildenafil on human aromatase activity: From in vitro structural analysis to catalysis and inhibition in cells
,Baravalle, R;Valetti, F;Catucci, G;Gambarotta, G;Chiesa, M;Maurelli, S;Giamello, E;Barone, I;Catalano, S;Andò, S;Di Nardo, G;Gilardi, G;,
J. Steroid Biochem. Mol. Biol.
,PubMed ID 27616271
[CYP19A1]
|
|
A common polymorphism in the human aromatase gene alters the risk for polycystic ovary syndrome and modifies aromatase activity in vitro
,Hui Wang, Qiaoli Li, Teng Wang, Guizhong Yang, Ying Wang, Xiaoping Zhang, Qing Sang, Huan Wang, Xinzhi Zhao, Qinghe Xing, Juanzi Shi, Lin He, and Lei Wang,
Mol. Hum. Reprod., Jun 2011; 17: 386 - 391
[CYP19A1]
|
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Expression of the K303R Estrogen Receptor- Breast Cancer Mutation Induces Resistance to an Aromatase Inhibitor via Addiction to the PI3K/Akt Kinase Pathway
,Ines Barone, Yukun Cui, Matthew H. Herynk, Arnoldo Corona-Rodriguez, Cinzia Giordano, Jennifer Selever, Amanda Beyer, Sebastiano Andò, and Suzanne A.W. Fuqua,
Cancer Res., Jun 2009; 69: 4724 - 4732.
[CYP19A1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205890 | CYP19A1 (Myc-DDK-tagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1 |
CNY 3,744.00 |
|
| RC205890L1 | Lenti ORF clone of Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1, Myc-DDK-tagged |
CNY 6,144.00 |
|
| RC205890L2 | Lenti ORF clone of Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205890L3 | Lenti ORF clone of Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205890L4 | Lenti ORF clone of Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1, mGFP tagged |
CNY 6,144.00 |
|
| RG205890 | CYP19A1 (tGFP-tagged) - Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1 |
CNY 5,344.00 |
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