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SESN1 (NM_014454) Human Untagged Clone
CAT＃: SC127795
SESN1 (untagged)-Human sestrin 1 (SESN1), transcript variant 1
CNY 6,168.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PA26; SEST1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127795 sequence for NM_014454 edited (data generated by NextGen Sequencing)
ATGGCTGAAGGAGAGAATGAAGTGAGATGGGATGGACTCTGCAGCAGAGATTCAACTACT AGGGAGACAGCATTGGAAAACATTAGGCAAACCATTTTGAGGAAAACCGAGTATCTTCGT TCGGTGAAAGAAACACCTCATCGTCCATCAGACGGGCTTTCAAATACCGAGTCTTCGGAT GGGTTGAATAAGCTACTTGCTCATCTGCTTATGCTTTCTAAGAGGTGTCCCTTCAAAGAT GTGAGAGAGAAAAGTGAGTTTATTCTGAAGAGCATCCAGGAACTTGGCATTAGAATTCCT CGACCACTAGGACAGGGACCAAGCAGATTCATCCCAGAAAAGGAGATCCTCCAAGTGGGG AGTGAAGACGCACAGATGCATGCTTTATTTGCAGATTCTTTTGCTGCTTTGGGCCGTTTG GATAACATTACGTTAGTGATGGTTTTCCACCCACAATATTTAGAAAGTTTCTTAAAAACT CAGCACTATCTACTGCAAATGGATGGGCCGTTACCCCTACATTATCGTCACTACATTGGA ATAATGGCTGCGGCAAGACATCAGTGCTCCTACTTAGTGAACCTGCATGTAAATGATTTC CTTCATGTTGGTGGGGACCCCAAGTGGCTCAATGGTTTAGAGAATGCTCCTCAAAAACTA CAGAATTTAGGAGAACTTAACAAAGTGTTAGCCCATAGACCTTGGCTTATTACCAAAGAA CACATTGAGGGACTTTTAAAAGCTGAAGAGCACAGCTGGTCCCTTGCGGAATTGGTACAT GCAGTAGTTTTACTCACACACTATCATTCTCTTGCCTCATTCACATTCGGCTGTGGAATC AGTCCAGAAATTCATTGTGATGGTGGCCACACATTCAGACCTCCTTCTGTTAGCAACTAC TGCATCTGTGACATTACAAATGGCAATCACAGTGTGGATGAGATGCCGGTCAACTCAGCA GAAAATGTTTCTGTAAGTGATTCTTTCTTTGAGGTTGAAGCCCTCATGGAAAAGATGAGG CAGTTACAGGAATGTCGAGATGAAGAAGAGGCAAGTCAGGAAGAGATGGCTTCACGTTTT GAAATAGAAAAAAGAGAGAGTATGTTTGTCTTCTCTTCAGATGATGAAGAAGTTACACCA GCAAGAGCTGTATCTCGTCATTTTGAGGATACTAGTTATGGCTATAAAGATTTCTCTAGA CATGGGATGCATGTTCCAACATTTCGTGTCCAGGACTATTGCTGGGAAGATCATGGTTAT TCTTTGGTAAATCGCCTTTATCCAGATGTGGGACAGTTGATTGATGAAAAATTTCACATT GCTTACAATCTTACTTATAATACAATGGCAATGCACAAAGATGTTGATACCTCAATGCTT AGACGGGCAATTTGGAACTATATTCACTGCATGTTTGGAATAAGATATGATGATTATGAC TATGGTGAAATTAACCAGCTATTGGATCGTAGCTTTAAAGTTTATATCAAAACTGTTGTT TGCACTCCTGAAAAGGTTACCAAAAGAATGTATGATAGCTTCTGGAGGCAGTTCAAGCAC TCTGAGAAGGTTCATGTTAATCTGCTTCTTATAGAAGCTAGGATGCAAGCAGAACTCCTT TATGCTCTGAGAGCCATTACCCGCTATATGACCTGA Clone variation with respect to NM_014454.2 >OriGene 5' read for NM_014454 unedited
ATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGCGGGGTGGGAACG CCGGAGAGAACCCGGTGGCTGCACAGACAAAAAAGCCCCGAATGGCTGGAGGGCGTTCAG CTGTTAACAGCCTTTTGGGGCAGAGCACGGATTTGACAGCTCCACAACGTGAGGATATCC ACTGACCCCGCGAGACGGAGGAGAACGCTTCCCCGAAATTCTCTGCCCACCAAAGCCAGC GCTGCAAGGTTGCAACTTTCAAACTTTGTTTTTCCAGAAAGAAGACTGCCCTTTCGTGTA CAAGGAGAGGGTGAGAGGGTGACCTANCTTGTAGATCGGCTGAAGGCACCAGTGGTTCCA AATGTCACCCAGATGTGTGTTTTCATGACGATTTGATTTCTCTGATTTTATTTTTACATT TTTCATTTTAAAAATACAAAGCAATTTTTTTGGGGCATGCTGAAAGGTAACTGAAGACCG CAAAGGAAAAACTATTGTCATGGCTGAAGGAGAGAATGAAGTGAGATGGGATGGACTCTG CAGCAGAGATTCAACTACTAGGGAGACAGCATTGGAAAACATTAGGCAAACCATTTTGAG GAAAACCGAGTATCTTCGTTCGGTGAAAGAAACACCTCATCGTCCATCAGACGGGCTTTC AAATACCGAGTCTTCGGATGGGTTGAAATAGCTACTTGCTCATCTGCTTTATGCTTCTAA AGAGTGTCCCCTTCAAAGATGTGAGAGAGAAAAGTGAGTTTATTCTGAAGAGCATCCAGG AACTTGGCATTAGAATTCCTCGACCACTTAGACAGGGACCCAAGCAGATCTTCCCAGAAA GGAGATCTNCCAGTGGGGAGTGAAGACCCCAGATGCATGCTTTATTGGCC >OriGene 3' read for NM_014454 unedited
CGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGGAGAAAAATCTTCTTTATTTAAAA ATACCAGTAATACTGACAGACTTCAAAAGCAATTCACGCTTCCAGAATACAAAGTACTTA ATACATATTTTCAAACCTGTTTGCATTTCAAACAAAGTTAGCGTTTTTGTAAATCAAATT TGATAACCCGACTAAAAATATTTTCCAGCTTTATTATTTAAAGAGCTGCACAGCCTTTAA AGTGGGGACCAGGAGGCAGGCAGAGGCAGATAGACTGAATGCACCCAGGACTGCGCAGCA GTCTACAGCAACATGTCCCACAACTTTGGTGCTGGAAACACAAGTAATGCACAAGACAGC TGCCCTCCAGTGTCAGGATCCTGTGAAACAGCATATCAAAAGATCGCCAGCTTCTTATAA TTTACACACTTTCATTTAAGATTGCTTTTTGAAGAAAATCTTTAAGAATGCCATTTTAAT TTAATATCCAGAACCCTGAATTTAAAAAACTAATGAAAGAAATTAACTGTTACCATCAAA ATGGTTGTTGCTGAGCTGTGTGAGTAAGAATTCTATGCTTTTTTAGATGCAAGAAAATTA ACACATAATGCAAGTTTAATTTGAAATCACACAAGTTATGTATGTAAGCTCAGATAGTAG CAATGTTCACTTTTATTACTAATAGCTTAACTGAAACAGCCATCTATTCATTCCTNCAGC AGGAATAGCAGCACCAAGATCTTCTAAAATCCTTTACATATAAAAGCTAAGTCATTTATC CAGTCCCATTCCTTTGCATGCTCCAATTCTTGTTATCTATTTAACATAAAAGCACAGGAT TTGGAGAGTTACTATTTGCTGTTTAAAACAGTTCCCAGCATTTGGACTGTCAAAAGCAAA ACTGGGATGGCACCCGNCTTCAGTCTAACCTGAACCGCCAAAGCACTGCTGGGCCACAAG TGNTTCACGAAAATTCCGATCTAAGATTTGCCCATGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014454 |
| Insert Size | 3090 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014454.1, NP_055269.1 |
| RefSeq Size | 2785 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 27244 |
| UniProt ID | Q9Y6P5 |
| Domains | PA26 |
| Protein Pathways | p53 signaling pathway |
| Gene Summary | This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities
,Pulito, C;Mori, F;Sacconi, A;Goeman, F;Ferraiuolo, M;Pasanisi, P;Campagnoli, C;Berrino, F;Fanciulli, M;Ford, RJ;Levrero, M;Pediconi, N;Ciuffreda, L;Milella, M;Steinberg, GR;Cioce, M;Muti, P;Strano, S;Blandino, G;,
Cell Discov
,PubMed ID 28698800
[SESN1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211305 | SESN1 (Myc-DDK-tagged)-Human sestrin 1 (SESN1), transcript variant 1 |
CNY 6,152.00 |
|
| RC211305L3 | Lenti-ORF clone of SESN1 (Myc-DDK-tagged)-Human sestrin 1 (SESN1), transcript variant 1 |
CNY 6,650.00 |
|
| RC211305L4 | Lenti-ORF clone of SESN1 (mGFP-tagged)-Human sestrin 1 (SESN1), transcript variant 1 |
CNY 6,650.00 |
|
| RG211305 | SESN1 (tGFP-tagged) - Human sestrin 1 (SESN1), transcript variant 1 |
CNY 7,752.00 |
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