Guanylate kinase (GUK1) (AL832986) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GMK
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832986, the custom clone sequence may differ by one or more nucleotides CGGACGCGTGGGGCGGACGCGTGGGATCACAGATAGGAGTCCCAAGTCCTATGAGAAGTTCCTGGAATAG GTGTAGATTCAGTAGATCTTTACAAGACCATATCTGCAGGGCAAGGTACCAGAGGACAGAGGCGGGGACA GGGACACTTCCATTCCAGACCTAGCAGCCCAGCACTCAGCACCATGCATGGGAGCAAATGGCTGGACTCC TGGGTGGGGTGGGGGTCTCAGAGCAGGCTCCCAGAGGGCTTGGAGGTGACTCCACCAGGTGGGGACGGCA GCTCCCAGGTAGGGTGTCATCAGAGTAGACAGCATTGCTTGCTAGGGACCCCTGGGGAGGCTGACAGGGT CAGTGGGTTTCAGTTGGGGGGCTCCCCTGCTGAGAACCCAGTAAAGCCGGCCTTCCATTCGTCTCCCGTG TGCCCAGAGCCTGGTCTGAGGGCCGCCCTGTGCATGCCGGCCCTTCCAACGTGGCAGAGCTCAGGGGGAA GAACACCCAGGCTCTCAGGAGACTCTCAGGCCAATGTCTCCATCCCTGGGTCAGCCCTTTCCTGCCATGA ATTCAGGAAGGCAGAGGCAGCTCAGCAGATGGGGACTAGAGGCCGCACTGCTATCCACAGCCTCTCTTCT CACCCCCAGGCATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACCCT GCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCGTGAGTCCAGGGCTCTC GTGGAGGGGTGCGTAGACCTCAAGGCTGCTGAGTAGTCCTAGCACCGTGAGCAGGCCAGGAGCCCAAACC CAACAGGCACACCCACCCTGCAGACTGTCCGAACTCTTGCACACTCCCCCCCACACAGAACCTGAGGTTA TCACACTCCTGCTGTCCTGCGTGCCTGTGTCTCCCTTCCCTGGGTCTGTTGAGTACTGATAACTGGGCCA CAGTGTTTCTTTCTGGGAGAACCCTCGCCTTGTAGGCTCCTGCGCCTTCCCAGTGGTGTGCTTCACTGGC TGCCTGCATCCTGGGGCTCAAGTGCTGTCGGGACTGCAAGGGAAACGCTGGGTGGGGCATTGGGCTCCGA GCAGCCCCCGATGGGTGACAGGTCTCTCTGCTAGATACCACGAGGAACCCGAGGCCCGGCGAGGAGAACG GCAAAGGTGAGTGGGGTGGGGCCCTATGGCTGGAGCACCCCCAGTGTGGGCAGGGCTGCTGGGCCCTGCA GCTGTGTTGGCTGTGCTGCCCGTCTCCTGCCCCCATCAATCCCTAATCTGTGAGATGGGTCCTTGCCTCC AAGGGCCGGTGAACTCAATCAGGGTGTCAGCGCCACAGCGTGGTGTCGCCTTCCTTGGGTACAGTGTGAG AGGCCGGCCAAGGCCTGGGGCTGTCTTCCTCCCACCTTGGAGGCGGCCACAGTGCTGCTGTCCCCAGCCC TGTCCTGGACTCGGCACTTATCAGCACTTTTGAGCTGTCTTCTGGGGTCCTGGTAAAAAGGGCTACTCTG CCTGCCTGATTCAAGACAAGGGACCCCCTTCCCAACAGCACCCCCGCCCCTTGCCGTGCAACCCAGTGGT CTCCAGTCACCCCACCACATCGTCCCCTCTGTAACCTGACGGTCTCCAGTTCCCCCACCACCTTCCCCCA GAACCTGTTGGTCTCCAGTCCCCACCCCATCACCACCAACTCCCAACTCCCCACTGGAACCCAGCAGTCT CGTATCTCCATCAGTGAGGACAGTGTGAGAAATGGTGTCTGGCTCAGGCACTTGGCAGCACTTGAGGGGT CCTCAGATGTCTCCTGCCCAGCAAGGCTGACTAAAGCAGTCATGGGTGTGGGAGGGGCCTGCAGGCATGC CTGGGTTGGGGGCAGCTGGCCCTGGGCACCCTGGTGCAGGTCCAGTCTGCCCTCTGGATGGCCCCTCCTC TTCCCCAGATTACTACTTTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAG CATGCCGAGTTCTCGGGGAACCTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACC GCATCTGTGTGCTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTA CATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACTGAAACCGAG GAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGAAATCAAGAAAGTTCAAAGG ACCGGCGCCTGAGGCTTGCTGTCTGTTCTCGGCACCCCGGGCCCATACAGGACCAGGGCAGCAGCATTGA GCCACCCCCTTGGCAGGCGATACGGCAGCTCTGTGCCCTTGGCCAGCATGTGGAGTGGAGGAGATGCTGC CCCTGTGGTTGGAACATCCTGGGGTGACCCCCGACCCAGCCTCGCTGGGCTGTCCCCTGTCCCTATCTCT CACTCTGGACCCAGGGCTGACATCCTAATAAAATAACTGTTGGATTAGAAACTCCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832986 unedited GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGTGGCCCC GGATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCCCCACCGG ACGGCATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCA CCCTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCGTG AGTCCAGGGCTCTCGTGGAGGGGTGCGTAGACCTCAAGGCTGCTGAGTAGTCCTAGCACC GTGAGCAGGCCAGGAGCCCAAACCCAACAGGCACACCCACCCTGCAGACTGTCCGAACTC TTGCACACTCCCCCCCACACAGAACCTGAGGTTATCACACTCCTGCTGTCCTGCGTGCCT GTGTCTCCCTTCCCTGGGTCTGTTGAGTACTGATAACTGGGCCACAGTGTTTCTTTCTGG GAGAACCCTCGCCTTGTAGGCTCCTGCGCCTTCCCAGTGGTGTGCTTCACTGGCTGCCTG CATCCTGGGGCTCAAGTGCTGTCGGGACTGCAAGGGAAACGCTGGGTGGGGCATTGGGCT CCGAGCAGCCCCCGATGGGTGACAGGTCTCTCTGCTAGATACCACGAGGAACCCGAGGCC CGGCGAGGAGAACGGCANAGGTGAGTGGGGTGGGGCCCTATGGCTGGAGCACCCCCAGTG TGGGCAGGGCTGCTGGGCCCTGCAGCCTGTGTGGCTGTGCTGCCCGTCTCCTGCCCCCAT AATCCNTAATCTGTGAGATGGGTCCTTGCCTCCAGGGCCCGTGAACTCCATCAGGGTGTC AGCGCCCCAGCGTGGTGTCGCCTTCCTTGGGTACAGTGTGAGAAGGCCGCCAGGCCTGGN GCTGTNCTCCTCCCACCTGNNAGNGNCACAGTGCTGCTGTCCCCAGCCTGTCCTGGAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL832986 unedited GCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTAATCCAACAGTTATTTTATTAGGA TGACAACCCTGGGTCCAGAGCGAGAGATAGGGACAGGGGACACCCCACCGAGGCTGGGTC GGGGGTCACCCCAGGATGTTCTAACCATAGGGGCAGCATATTCTTCACTTCACATGCTGC CCAAGGGCACTCCACCTGACGTCATCATCTCCCAAGGGGCCGCCCTCAATGCTCCTGCAT TGAATCTGAATGGCCCTGGTGTGTCCCCACCCTCACCTCCCCCACTCGTTACATATCTCT TACTTCCCGTCCACTCCCTTCATAATCCCCCTTCCCTCCCCCCCCTCCCCCCCACTTCCC CTTCCTCTCTTTATCATTTTTTTACCCCGTCCTCCCTACCCTTTTTTTCCTACTCCCTCC TTTGGTCTTACTCCCATTATTCCACCCCCCTCTCCCCTATCCCCTCTTCTCTACCCAATC CGACCCCCCTATTTCCCACCACCCTATAAATACCCCCCTTCCCCTTTTCTATACCACCAC CTCCCCTTTCTGCCACTTATTCTTTCCTCAACTCTCCCCCTCCCCATTCCCATCCTTCTT CCTTACCCACCTCTTTGCCCACACCACTCCCGAATTACATCCTCCTCCCCTTTCTTTTTT CCCCCCCCTCCTTCCGCGCCGTCCTACCCATCATTCTACACCCTGGACACCCTACTTTCC CTCACCATTTTTATCCCCTTCTACCAACCACCCGCCCATTTCCCGCCCATACCATCATCT TACCCAGCCCCAATTCCCCCACAAAACACTTCCTCCTTCCCCATTTCCCATTTTATCCTC CTCACTCTTCCACCCTTCCTCTACTACCTTTTTCCACCCCCCCCCCTCTTTTCCTCCCCT CCTCTAATCTATATGTTCTACTATTCTACCCACCCCTCACTCCCACCCCCGATACCCCGT CNTACTATACCCCCTTCCCCAATTCTTTTCCTTCCCCCCTTTTCTCTTCCCATTTCCCCT CCT
Restriction Sites	NotI-NotI
ACCN	AL832986
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832986.1, CAH56207.1
RefSeq Size	2590 bp
RefSeq ORF	2590 bp
Locus ID	2987
Domains	Guanylate_kin, GuKc
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Purine metabolism
Gene Summary	The protein encoded by this gene is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.