MCFD2 (AK095006) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	F5F8D; F5F8D2; LMAN1IP; SDNSF
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK095006, the custom clone sequence may differ by one or more nucleotides CCACAGTCCAAAGTCTCATCTGAGACAAGGCAAGTCCCTTCCACCTATGAGCCTGTAAAATCAAAAGCAA GCTAGTTACTTCCTAGATACCAACAGGGGTACAGGTATTGATTAAAGACGGCTGTTCCAAATGGGAGAAA TTGGCCAAAATAAAGGGGTTACAGGGCCCATGCAAGTCCGAAATCCAGCAGGGCTGTCAAATTTTAAAGT TCCAGAATAATCTCCTTTGACTCCAGGTCTCACATCCAGGTCATACTGATGCAAGAAGTGGGTTCCCATG GTCTTGGGCAGCTCTGCCCCTGTGGCTTTGTAGGGTACAGCCTCCCTCCTGGCTGCTTTCACGGCTGTTG TTCAGTGCCTGCGGCTTTTCCAGGTGCACGGTGCAAGCTGTTGGTGGATCTACCATTCTGGGGTCTGGAG GACGGTGGCCCTCTTCTCACAGCTCCACTAGGCAGTGCCCCAGTAGGGACTCTGTGTGGGGGCTCCCACA CCACATTCCCCTTCTGCACTGCCCTAGCAGAGGTTCTCTCCCCTGCCGCTGAGAGGGCCTCTCCCCTGCA GCAAACGTTTGCCTGGGCATTGAGGCATTTCCATACATCTTCTGAAAACTAGGCGGAGGTTTCCAAATCT CAATTCTTGACTTCTGTGCACCTGCAGGCTTAACAGCACATAGAAGCTGCCAAGGCTTGGGGCTTCCACT CTGAAGCCACAGCCCGAGCTGTATGTTGGCCCCTTTCAGCCATGGCTGGAGTGGCTGGGACACAAGACAC CAAGTCCCTAGGCTGCACACACATGTCAGGGGCTGCCCTGACATGGCCTGGAGACATTTTCCCCATGGTG TTGGGGATTAACATTAGGCTCCTTGCTACTTATGCAAATTTCTGCAGCTGGCTTGAATTTCTCCCCAGAA AATGGGTTTTTCTTTTCTATTGCATAGTCAGGCTGCAAATTTCCAAACTTTTATGCTTTGCTTCCCTTAT TTATAAGGGAATGCCTTTAAAAGCACCCAAGTCACCTGTTGAACACTTTGCTGCTTAGAAATTTCTTCCG CTAGTTAACCTAAATCATCTCTCTCAAGTTCAAAGTTTCACAAATCCCTATGGAAGGGGCAAAATGCTGC CAGTCTCTTTGCTAAAACATAACAAGAGTCACCTTTACTCCAGTTCCCAACAAGTTCCTCATCTTCATCT GAGGCCACCTCAGCCTGGACTTTGTTGTCCATATTGCTATCAGCATTTGGGGCAAAGCCATTCAACAAGT CTGTAGGAAGTTCCAAACTTTCCCACATTTTCCTGTTTTCTTCTGAGCCCTCCAAACTGTTCCAGCCTCT GCCTGTTACCCAGTTCCAAAGTCACTTCCACATTTTGGGTATTTCTTCAGCAGGTCCCAATCTACTGGTA CCAATTTACTGTATTAGTCCGTTTTCACGCTGCTGATAAAGACATACCCGAGACTGGGAAGAAAAAGTGG TTTAATTGGACTTAAAGTTCCACATGGCTGGGGAGGCCTCAGAATCATGGTGGGAGGCAAAAGACACTTC TTACATTGTGGCAAGAAAAAATGAGGAAGAAGCAAAAGCAGAAACCCCTGATAAACTGATCAGATCTCAT GAGACTTATTCACTGTCACGAGAATAGCACGGGAAAGACTGGCCCCCATGATTCAATTACCTCCCCCTGG GTCTGTCCCACAACACGTGGGAATTCTGGGAGATACAATTCAAGTTGAGATTTGTGGGGGGACACAACCA AACCATATCAGCATCCTTTCAAGAATATTAGATAATTGGAGCTGAGTACTCAGGAACTTGACTGTAGTAG AATACCGCTAGTTTCTTAATTTTAATTCACATCACCTGAAAAGTAAAACAACAGGCTTTGCCAAGTGGAT GCTTTTCAGTAACAGTGAAGTGGAGTGAATACCAAATGTTTGCCCTGGTGGTTCCTATCTCTTCAGGCAA ACATGGTCAGTATTCTGTAAAGTTCCCCTGGCCTAAATGATTACTTGCTCTGGGCAAGTGGATATTTATT AGGCTATTTCAAAGCCACAGCATAAGAATGTCAGCCTAGCCACAGAGTCTGAGATTCTGAGTTCAGCCTA GCCACAGAGTCTAAGATTCTGTATCCTCTGACATTTTGGAAATGATACACTACTGGCTTAAGTGATGACT CTTTCAGATTTTCAGTATTTTATACAACTACTGCCACATCCTTATACTTTATTGCTTTTCTGTCTTCTTC AACCTGGGAGAGACCCTGAATTTGAGTGTGTTCTCTAATCAATAGTGGTTTAGCTTTCTTTTCTATTTCA CTCGTTTCTAGGGTTTTTTATTTGCAGTTTAGGAACTACTAGGAATGTCAGGACTTTATCAGCAGGGGTA AAACTACCACCTGGCCTAGCCTAAGTAGGAAGTGAAAAGATAATTCACCAAACAATGATTAATCAGATAG AAGTTCTAGTCAAGAGGGATATTGTTGAAGTTACCTCTTTTAGCCTAGATACATGGATTCTTTTCAAATC AGGAAAGATTAGAAAAGGAACCCAAAAAACCCTTTAACAGTGTGAATCTTTATAGTATTTGAAAATGAGA AGAAGCAGCAGATTGTAATTTGGTTTATTGGATGTGATGGACGTTCTGTAATAGAAAACCTGAAACGATG ATTGAATGGGAAAAAGAGACTACAAAATTTGTCGTAGGATGTATACAGACTTATTTTCTTTATTACAGTA TTATAAGAAAACATATGTATTTGTAAAAATGGTTTCCTGTGTCAAGTATTTGTGCAGTCAGAGCTGACTT GTAAACTATTCTTGTAATAGCTCATTATTTTGAAAGATTTATATATGATGAATTCTGGATATATGACCAA TAAAACTGATGAAGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095006 unedited GAATTCGGCACGAGGAGAAATTGGCCAAAATAAAGGGGTTACAGGGCCCATGCAAGTCCG AAATCCAGCAGGGCTGTCAAATTTTAAAGTTCCAGAATAATCTCCTTTGACTCCAGGTCT CACATCCAGGTCATACTGATGCAAGAAGTGGGTTCCCATGGTCTTGGGCAGCTCTGCCCC TGTGGCTTTGTAGGGTACAGCCTCCCTCCTGGCTGCTTTCACGGCTGTTGTTCAGTGCCT GCGGCTTTTCCAGGTGCACGGTGCAAGCTGTTGGTGGATCTACCATTCTGGGGTCTGGAG GACGGTGGCCCTCTTCTCACAGCTCCACTAGGCAGTGCCCCAGTAGGGACTCTGTGTGGG GGCTCCCACACCACATTTCCCTTCTGCACTGCCCTAGCAGAGGTTCTCTCCCCTGCCGCT GAGAGGGCCTCTCCCCTGCAGCAAACTTTTGCCTGGGCATTGAGGCATTTCCATACATCT TCTGAAACTAGGCGGAGGTTTCCAAATCTCAATTCTTGACTTCTGTGCACCTGCAGGCTT AACAGCACATAAAAGCTGCCAGGCTTTGGGGCTTCCACTCTGAACCACAGCCNAGCTGTA TTGTGGGCCCTTTCACCCTGGGTTGAAGGGGCTGGGACACAGAACCAAGCCCTAGGCTGA CACCCATGTAAAGGGCTGCCCTGCATGCCTGAAACATTTCCCCGGGGTTGGGGGATAAAC TTAGGCTCTTGCTCTAAGCAAATCTGGGCCGGGCTGAATTC
Restriction Sites	NotI-NotI
ACCN	AK095006
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095006.1
RefSeq Size	2885 bp
RefSeq ORF	2885 bp
Locus ID	90411
Gene Summary	This gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LMAN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in adult central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2016]

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