BAF53A (ACTL6A) (NM_178042) Human Untagged Clone

CAT#: SC127725

ACTL6A (untagged)-Human actin-like 6A (ACTL6A), transcript variant 3



  "NM_178042" in other vectors (4)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-ACL6A antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC127725 sequence for NM_178042 edited (data generated by NextGen Sequencing)
ATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAA
CAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATG
GAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATT
TTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATG
TCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAA
CACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAAT
GGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTC
CACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATT
ACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATG
ATTGCATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAG
TTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTT
CAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATG
CCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAGCGGCTA
AAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATG
TTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGATATCAGACCA
GGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGAC
AGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCA
AATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCT
TTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAG
TGTGTAGAAAGAAAATGCCCTTGA

Clone variation with respect to NM_178042.2
891 c=>t
>OriGene 5' read for NM_178042 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGAGCTCCGGGGTGTGTGGA
CGCCGCTTTGTTGCCTGAGGTGGGTGGCGGTGGAAGTTAAGGGAGTCAGGGGCTATCGCT
CCTCGAGACTCGCAGTCGCGGCCACTGCAGTCACTTCGCCAGTTAGCCCTTAGGGTAGGA
GTCGCGCCGGCAGCAGCCATGAGCGGCGGCGTGTACGGGGGAGATGAAGTTGGAGCCCTT
GTTTTTGACATTGGATCCTATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAG
GTGGATTTTCCTACAGCTATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATG
GAAATAGATGGCGATAAAGGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCT
CTGCGTGTTCCGAGGGAGAATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAA
GACTGGGATAGTTTCCAAGCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAA
GCCAGTCTCCATCCTGTTCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAG
AAACTGACAGAGTTAATGTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAANACT
GCAGTTTTGACAGCATTTGCTAATGGGTCGTTCTACTGGGGCTGATTNTGGNNACAGTGG
AGCCCACTCATACCACTGCAATTCCAGTCCACGATGGGCTATGTCCTTCAAACAGGCATT
GTGAAAATCCCTCTTGCTGGAGACTCTATTCTATGCAGGGCAGAGAACTCTTCCAGAATG
ATATGGGGATTTGGCTCCTCCTATATGATAGCTCAAAGGAGCTGCTCGGGGAGGATCTCA
GCAACTGGAAGAAAANGAGAGCTGCTCAGGTTCCGAGGGCTTGCACATATATGGGGAATT
GGGTANCCAGGATTCAG
>OriGene 3' read for NM_178042 unedited
TTATGTCCGTGGCCCGCAATCTACGACCGAGTTTTTTTTTTTTTTTTTTATTTCTGGCAA
GGCTAATTTTTATTTAATTCTGCAAGCCTAAGGCAAAAAGCATAGGCAGCAACTTTTACT
AGTCAATAAAAAGCAGTTCTACCAATCCACTGGTAATTAATACACTAAACAGAGTTGGAA
AGCATTTTACTGAAAGCAAAATATTTAGAGAAAATAGCACATTTATACAAAATTATAAAA
TGCTTGTAATAACAATAAGCGCATTTTAAGGAAAGCACAAACTTAATTTATAGAGCCAGG
TCAAGCTTTAAAAAATTTAAGTGCAAATTGAAATATGCACAAATGTATAAACATTCTACA
AAAGATGGCCATTCTTTTCCTGAGTATACTAACGCTATGAAACCCACGGCGACACAAGGA
AGGTCCAACCTTGGGAACTCTTTCTCAAGGGCATTCCGTTTATACACACTGCTTAACTAC
TTGTTAATCAACCGGCTTGAAAACCCACATTTGTTCCAAGGTACTCAACCAGGTTACAAT
GGTCTGCCTAACCTATGATATAAACCTCCGCAGACTGCCGCATGATCTCGACCCCTCTTA
CTCGTTACTTGCTGGATTTTTTTGTACCCTCCTCTATCCCACCTGCTATTAACCCCCTGC
ATCACAGACCACCCCCCCCCCCTATCACCCTGCGTAGACCACTGCCCGCATTTGATTTTC
CCCTGTCCACACCTGCGGCGACACCGTGACTGACTGTTAAACTGTAGCTTCGCGAAACAC
CTTACATGGCAAGGCCGTACAACCCTCCGCCACCTCTGACCTCTTTTGCTCCACTCCCCC
CCGCCCCGCNGGACGACCCATACCGCCCCGCCAGCCTCCCGGCGACGCTTGGCTTAATTC
ACCCGTACCGCCCCTGGCGCGCCCTCGCTCCCTAACCGCGTATACCCTCGGCGCGCTCAT
CGCTCCCGACTGGCTAGTGTG
Restriction Sites NotI-NotI     
ACCN NM_178042
Insert Size 1950 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_178042.1, NP_829888.1
RefSeq Size 1925 bp
RefSeq ORF 1164 bp
Locus ID 86
UniProt ID O96019
Protein Families Druggable Genome, Transcription Factors
Gene Summary This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region that results in the use of a downstream start codon, compared to variant 1. Variants 2 and 3 both encode isoform b, which has a shorter N-terminus when compared to isoform a. This variant is also known as Nbeta3 and isoform b is also known as hArpNbetaS. Isoform b is found mostly in the cytoplasm.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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