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BAF53A (ACTL6A) (NM_178042) Human Untagged Clone
CAT＃: SC127725
ACTL6A (untagged)-Human actin-like 6A (ACTL6A), transcript variant 3
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127725 sequence for NM_178042 edited (data generated by NextGen Sequencing)
ATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAA CAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATG GAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATT TTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATG TCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAA CACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAAT GGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTC CACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATT ACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATG ATTGCATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAG TTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTT CAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATG CCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAGCGGCTA AAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATG TTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGATATCAGACCA GGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGAC AGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCA AATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCT TTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAG TGTGTAGAAAGAAAATGCCCTTGA Clone variation with respect to NM_178042.2 891 c=>t >OriGene 5' read for NM_178042 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGAGCTCCGGGGTGTGTGGA CGCCGCTTTGTTGCCTGAGGTGGGTGGCGGTGGAAGTTAAGGGAGTCAGGGGCTATCGCT CCTCGAGACTCGCAGTCGCGGCCACTGCAGTCACTTCGCCAGTTAGCCCTTAGGGTAGGA GTCGCGCCGGCAGCAGCCATGAGCGGCGGCGTGTACGGGGGAGATGAAGTTGGAGCCCTT GTTTTTGACATTGGATCCTATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAG GTGGATTTTCCTACAGCTATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATG GAAATAGATGGCGATAAAGGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCT CTGCGTGTTCCGAGGGAGAATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAA GACTGGGATAGTTTCCAAGCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAA GCCAGTCTCCATCCTGTTCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAG AAACTGACAGAGTTAATGTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAANACT GCAGTTTTGACAGCATTTGCTAATGGGTCGTTCTACTGGGGCTGATTNTGGNNACAGTGG AGCCCACTCATACCACTGCAATTCCAGTCCACGATGGGCTATGTCCTTCAAACAGGCATT GTGAAAATCCCTCTTGCTGGAGACTCTATTCTATGCAGGGCAGAGAACTCTTCCAGAATG ATATGGGGATTTGGCTCCTCCTATATGATAGCTCAAAGGAGCTGCTCGGGGAGGATCTCA GCAACTGGAAGAAAANGAGAGCTGCTCAGGTTCCGAGGGCTTGCACATATATGGGGAATT GGGTANCCAGGATTCAG >OriGene 3' read for NM_178042 unedited
TTATGTCCGTGGCCCGCAATCTACGACCGAGTTTTTTTTTTTTTTTTTTATTTCTGGCAA GGCTAATTTTTATTTAATTCTGCAAGCCTAAGGCAAAAAGCATAGGCAGCAACTTTTACT AGTCAATAAAAAGCAGTTCTACCAATCCACTGGTAATTAATACACTAAACAGAGTTGGAA AGCATTTTACTGAAAGCAAAATATTTAGAGAAAATAGCACATTTATACAAAATTATAAAA TGCTTGTAATAACAATAAGCGCATTTTAAGGAAAGCACAAACTTAATTTATAGAGCCAGG TCAAGCTTTAAAAAATTTAAGTGCAAATTGAAATATGCACAAATGTATAAACATTCTACA AAAGATGGCCATTCTTTTCCTGAGTATACTAACGCTATGAAACCCACGGCGACACAAGGA AGGTCCAACCTTGGGAACTCTTTCTCAAGGGCATTCCGTTTATACACACTGCTTAACTAC TTGTTAATCAACCGGCTTGAAAACCCACATTTGTTCCAAGGTACTCAACCAGGTTACAAT GGTCTGCCTAACCTATGATATAAACCTCCGCAGACTGCCGCATGATCTCGACCCCTCTTA CTCGTTACTTGCTGGATTTTTTTGTACCCTCCTCTATCCCACCTGCTATTAACCCCCTGC ATCACAGACCACCCCCCCCCCCTATCACCCTGCGTAGACCACTGCCCGCATTTGATTTTC CCCTGTCCACACCTGCGGCGACACCGTGACTGACTGTTAAACTGTAGCTTCGCGAAACAC CTTACATGGCAAGGCCGTACAACCCTCCGCCACCTCTGACCTCTTTTGCTCCACTCCCCC CCGCCCCGCNGGACGACCCATACCGCCCCGCCAGCCTCCCGGCGACGCTTGGCTTAATTC ACCCGTACCGCCCCTGGCGCGCCCTCGCTCCCTAACCGCGTATACCCTCGGCGCGCTCAT CGCTCCCGACTGGCTAGTGTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_178042 |
| Insert Size | 1950 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178042.1, NP_829888.1 |
| RefSeq Size | 1925 bp |
| RefSeq ORF | 1164 bp |
| Locus ID | 86 |
| UniProt ID | O96019 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region that results in the use of a downstream start codon, compared to variant 1. Variants 2 and 3 both encode isoform b, which has a shorter N-terminus when compared to isoform a. This variant is also known as Nbeta3 and isoform b is also known as hArpNbetaS. Isoform b is found mostly in the cytoplasm. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205341 | ACTL6A (Myc-DDK-tagged)-Human actin-like 6A (ACTL6A), transcript variant 3 |
CNY 3,656.00 |
|
| RC205341L3 | Lenti ORF clone of Human actin-like 6A (ACTL6A), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205341L4 | Lenti ORF clone of Human actin-like 6A (ACTL6A), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG205341 | ACTL6A (tGFP-tagged) - Human actin-like 6A (ACTL6A), transcript variant 3 |
CNY 4,370.00 |
