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Epac2 (RAPGEF4) (NM_007023) Human Untagged Clone
CAT＃: SC127722
RAPGEF4 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1
CNY 10,568.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_007023, the custom clone sequence may differ by one or more nucleotides
ATGGTCGCTGCGCACGCTGCCCATTCTTCCTCCTCTGCCGAGTGGATCGCCTGCCTGGATAAAAGACCAC TGGAGCGATCCAGCGAAGATGTGGATATAATCTTCACTCGACTGAAAGAAGTTAAAGCTTTTGAGAAATT TCACCCAAATCTCCTTCATCAGATTTGCTTATGTGGTTATTATGAGAATCTGGAAAAGGGAATAACATTA TTTCGCCAGGGTGATATTGGAACAAACTGGTATGCTGTCCTGGCAGGGTCTTTGGATGTTAAAGTATCTG AGACCAGCAGTCACCAGGATGCTGTGACCATCTGTACCCTGGGAATTGGGACGGCCTTTGGAGAGTCCAT TCTGGACAACACACCCCGCCATGCAACCATCGTTACCAGGGAGAGCAGTGAACTGCTCCGCATCGAGCAG AAGGACTTCAAGGCACTATGGGAGAAATATCGACAGTATATGGCAGGACTTCTGGCTCCTCCTTATGGTG TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAACACACCTCTCATTGAACCTCACGT TCCTCTTCGTCCTGCTAACACCATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTA CGAAATGCCATTCTCTCTCGAGCACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAGAC AATGCTGTGTGGGAACTGAACTGGTGGACTGGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCA AGCTGTTGGCATGTGGCAAGTCCTGTTAGAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTC CAAGACAAATATTTATTCTATCGATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGG AGAAGAAGGAGTGTGATGAGGAGCTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCA CATGAGGATGATCCTTCGCAAACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAG CTTCTTCATATTAAAGCCTTATCCCATCTTTCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTT TTGAGTCTCACGCCAAAGGAGGGACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTAT TCTAAAAGGATCAGTGAATGTAGTCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGAC TTCGGCAAGTTAGCACTAGTGAATGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCC ATTTCTTAAGAGTAGACAAGGAGGATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACT TAAAGAACATGACCAAGATGTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGA AACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAG AAACAATACGCCTTGAGGCAACTTTAAATGAAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCA CTGTGTTTTTATGCCAAATACCCAGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAA GGTACAGAACAGGAGAAAATGGATTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGT GGGCTGCCATGTATGGAGACCTCCTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGT ATCTGTATCAGATGATGCCCGGATGATTGCTGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTC AAGCAAATCTCAGAAGATGCAAAGGCACCACAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGG GCGATGAGAGAGCCCAGAAGCGCCAGCCTATCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCAT GGACCACACCTACACAACCATTCGGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCC GACAAGCTGGGCTCCGGGGAGGGCCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCA AACCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCA ATTCGATTCACTGACTCCCTTACCAGAACAGGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTG ATGAGCTCCAAAGATTTAGCATACCAGATGACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGC TGGAGCTAATCTATCACACATTTGGAAGGCATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCT GAGGAGATTTAATGAAATTCAGTTTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGT GTTCAGCTATTAAAAAAATTTATTAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTT TTGCCATCGTCATGGGACTAAGTAACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAG CAAGTTCAAGAAGTTCTATGCGGAGTTTGAAAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGG CTGACAGTAGCTAAGCTGGAACCTCCTCTCATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTTA CTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATAC GGCCAGAACAGTGAGATACTACAGGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCAT CAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCAC ACAGATTAGAGCCTCGTCGACCATAG |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | NotI-NotI |
| ACCN | NM_007023 |
| Insert Size | 4640 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007023.1, NP_008954.1 |
| RefSeq Size | 4278 bp |
| RefSeq ORF | 3036 bp |
| Locus ID | 11069 |
| UniProt ID | Q8WZA2 |
| Domains | DEP, cNMP, RasGEFN, RasGEF |
| Protein Pathways | Leukocyte transendothelial migration |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205335 | RAPGEF4 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1 |
CNY 10,560.00 |
|
| RC205335L1 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1, Myc-DDK-tagged |
CNY 12,960.00 |
|
| RC205335L2 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RC205335L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1, Myc-DDK-tagged |
CNY 10,550.00 |
|
| RC205335L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1, mGFP tagged |
CNY 10,550.00 |
|
| RG205335 | RAPGEF4 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1 |
CNY 12,160.00 |
