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MRCL3 (MYL12A) (NM_006471) Human Untagged Clone
CAT＃: SC127713
MYL12A (untagged)-Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3; MYL2B |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006471, the custom clone sequence may differ by one or more nucleotides
ATGTCGAGCAAAAGAACAAAGACCAAGACCAAGAAGCGCCCTCAGCGTGCAACATCCAATGTGTTTGCTA TGTTTGACCAGTCGCAGATTCAGGAGTTCAAAGAGGCCTTCAACATGATTGATCAGAACAGAGATGGTTT CATCGACAAGGAAGATTTGCATGATATGCTTGCTTCATTGGGGAAGAATCCAACTGATGAGTATCTAGAT GCCATGATGAATGAGGCTCCAGGCCCCATCAATTTCACCATGTTCCTCACCATGTTTGGTGAGAAGTTAA ATGGCACAGATCCTGAAGATGTCATCAGAAATGCCTTTGCTTGCTTTGATGAAGAAGCAACTGGCACCAT ACAGGAAGATTACTTGAGAGAGCTGCTGACAACCATGGGGGATCGGTTTACAGATGAGGAAGTGGATGAG CTGTACAGAGAAGCACCTATTGATAAAAAGGGGAATTTCAATTACATCGAGTTCACACGCATCCTGAAAC ATGGAGCCAAAGACAAAGATGACTGA >OriGene 5' read for NM_006471 unedited
GTCAGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTGCTCGGATT CTCGTAGCTGTGCCGGGACTTAACCACCACCATGTCGAGCAAAAGAACAAAGACCAAGAC CAAGAAGCGCCCTCAGCGTGCAACATCCAATGTGTTTGCTATGTTTGACCAGTCACAGAT TCAGGAGTTCAAAGAGGCCTTCAACATGATTGATCAGAACAGAGATGGTTTCATCGACAA GGAAGATTTGCATGATATGCTTGCTTCATTGGGGAAGAATCCAACTGATGAGTATCTAGA TGCCATGATGAATGAGGCTCCAGGCCCCATCAATTTCACCATGTTCCTCACCATGTTTGG TGAGAAGTTAAATGGCACAGATCCTGAAGATGTCATCAGAAATGCCTTTGCTTGCTTTGA TGAAGAAGCAACTGGCACCATACAGGAAGATTACTTGAGAGAGCTGCTGACAACCATGGG GGATCGGTTTACAGATGAGGAAGTGGATGAGCTGTACAGAGAAGCACCTATTGATAAAAA GGGGAATTTCAATTACATCGAGTTCACACGCATCCTGAAACATGGAGCCAAAGACAAAGA TGACTGAAATAACTTCAAATTCCAGCCAAACGTTCCTTGTTGCCACTTTGGGTATTCTGA GATTNTCTCTTGCATGCCCTTAGCTNTACAGCTTTTGCATTTCCTGTTGTATTTATTCTC AGCCATTNTGGGCATATGTATCTTTATAATCAGACTGGAAACGGGACTCTCTATTAATAT CATTTTCAGAATNAAAATAGGATAATTTAACCTACCAGCCCTTCTCCNCNCATACTGTGG TCTATACAGAGTCATATATCNTTTCAGAGAAAGTNATTCGCTCGATTCCTTCTGAATCAT TATTAAACTCTATGATAAAATAAAAAAAAAAAAAAACTCGCTCTAGATGCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006471 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006471.2, NP_006462.1 |
| RefSeq Size | 1243 bp |
| RefSeq ORF | 516 bp |
| Locus ID | 10627 |
| UniProt ID | P19105 |
| Domains | EFh |
| Protein Pathways | Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction |
| Gene Summary | This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) encodes the shorter isoform (1). Variants 1, 2 and 3 encode the same isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207294 | MYL12A (Myc-DDK-tagged)-Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A) |
CNY 2,400.00 |
|
| RC207294L1 | Lenti ORF clone of Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC207294L2 | Lenti ORF clone of Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), mGFP tagged |
CNY 5,890.00 |
|
| RC207294L3 | Lenti ORF clone of Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207294L4 | Lenti ORF clone of Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A), mGFP tagged |
CNY 5,890.00 |
|
| RG207294 | MYL12A (tGFP-tagged) - Human myosin, light chain 12A, regulatory, non-sarcomeric (MYL12A) |
CNY 4,000.00 |
