HIATL1 (MFSD14B) (BD140114) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD140114, the custom clone sequence may differ by one or more nucleotides GTGCATGCTTGTAATCGCAGCTACTTCGGAGCCTGAGAGACTCCTTCAGGGTGAGCAAAGGCCTGGAAAA ACCTGTATGCAGATAAAGAAAAGGAAAGAAAGAGATAATCAGTGCATGCAGTTGTCAGCTGGCTGGGACC TGAGGAGAGTCACTTGTGGAGGCAACTGGTCTTTATCCCCATTGTCCGGTACAAGGCAGGCATTAATCCT GTGATCCTTATCTGAAGCTCAGCTACAAGGCTTTGGCCGACCAAGTGTGTACCATGCTGCTATTGTCATC TTCCTTGAATTCTTTGCGTGGGGCCTGTTGACAACTCCAATGTTGACTGTTCTACATGAAACATTTTCTC AACACACATTCCTCATGAATGGTCTCATTCAAGGTGTAAAGGGCCTGCTCTCTTTTTTGAGTGCCCCACT CATTGGTGCCCTGTCTGATGTGTGGGGGAGGAAGCCCTTTCTCCTCGGCACTGTATTCTTTACCTGCTTC CCAATCCCACTGATGAGGATCAGCCCATGGTGGTATTTTGCGATGATTTCTGTGTCTGGAGTCTTCTCGG TCACGTTTTCTGTTATATTTGCCTATGTAGCTGATGTCACTCAGGAGCACGAGCGAAGTACAGCTTATGG ATGGGTCTCAGCCACCTTTGCGGCTAGTCTTGTCAGCAGCCCGGCCATTGGAGCATATCTTTCTGCCAGT TACGGAGACAGCCTCGTTGTGCTGGTGGCCACAGTGGTGGCTCTTCTGGACATCTGCTTCATCTTAGTGG CTGTTCCAGAATCTCTGCCTGAGAAAATGAGACCGGTTTCCTGGGGAGCTCAGATTTCTTGGAAACAAGC AGACCCTTTTGCGTCGTTGAAGAAAGTTGGAAAAGATTCTACTGTCTTACTAATCTGCATCACCGTGTTT CTTTCATACCTTCCTGAAGCTGGACAGTATTCAAGTTTTTTTCTCTATCTCAGGCAGGTCATAGGTTTTG GATCTGTTAAAATTGCAGCATTCATAGCTATGGTAGGAATTCTGTCTATTGTGGCTCAGACGGCCTTTCT TAGCATCTTGATGAGATCATTAGGAAATAAGAATACTGTCCTCCTTGGCTTGGGCTTCCAGATGCTCCAG TTAGCCTGGTACGGTTTTGGATCACAGGCCTGGATGATGTGGGCAGCAGGGACCGTGGCTGCCATGTCCA GCATCACGTTTCCGGCAATCAGTGCCCTCGTCTCTCGGAATGCAGAGTCAGATCAGCAAGGAGTTGCCCA GGGGATCATAACTGGAATAAGAGGACTATGCAATGGCCTGGGGCCAGCACTGTATGGCTTCATATTCTAC ATGTTCCATGTGGAACTGACTGAGTTGGGCCCGAAATTGAATTCTAACAACGTTCCCCTGCAGGGAGCTG TCATCCCAGGCCCGCCGTTTTTATTTGGGGCATGTATAGTCCTTATGTCTTTTCTGGTTGCCTTATTCAT TCCTGAATACAGTAAAGCCAGTGGAGTTCAAAAACACAGTAACAGCAGCAGCGGCAGCCTGACCAACACC CCAGAACGGGGCAGTGATGAGGACATTGAGCCACTACTGCAAGACAGCAGCATCTGGGAGCTCTCTTCAT TTGAGGAGCCTGGGAATCAGTGCACTGAGCTGTNAACTCGGCAGAAAGTGGGATTCTGCATACGCCATCT CTGAGAGCCATGGAGGGAGCCACACCCCTGGTGACTTCATGGTGCTGGATGGGAGACGCTAGCGGCATCC TTCAGGGCCAAGTTTGATAAATACCACCGCCATCATTCTGCTCATCCTCCTCCTGTTTTTTTTTTTTCTC TTACATTCTTTTTTTTTTTCCTGTTTATACATTAGAACAAGATAAGATTTGAAATACTTCCTTGCAAATA ATGTGCAACTCCCAAGGTGAAACTCAAATAGAAAAAGTCATCTCTCTGGTAGAAAGGATGGCTTTCCTGT AATGACTATAGAGTAAGAGTGGCAGCAATCTTTCCATGCCCTTTTCAGCAGAAGGCACAGAACAGTAGCG GGACTGCCATCTCTGGCAAGATTTCAGGTAAAGAATCTCTTCTTAATTTCTACCTTCCTGTTTCTCTGAA TCAGCCCATAGGTGTTGATGAGTGGCCACTCTTAAAGAGTCACTCAGTATCAGGGATCTACTGTCTTTGT TCAAAGGTCAAATAAAAACCTAGTCTCCTTTTATTCTACTTTCTATTCTTAGCTAGAATGAAACTCAGCA TATATACACTTCTGGACATAATAATATTGAATAGTAATTACCTTTACTAGATGAAAGAAATTTTCATTAC AAACTTAAATCATGTAAAACTCAACAACTCAGATTCCTGGACCTGGTGTCCTGGTTGGGTCCAAGGTGAT TTTACAGAAGAAAAAAACAACTCAAGCATTCTGGTGGCAACATAGAGATTGTAGGCTGCTTCTAAGAAAG TTATTAACAATTTGGAAATTCCTAAGTAGGATGAGAGTTAGTAACTGGATACGAGTGAAGTTTATATCCA AGTTCAGACTCAAAGGCATTATTATGATTTGCTTCTTCCCATGTCTTCCATGTCCTGCTTCTCAAAGTTT TTCTCATCCATCACACTCCTGCCTTAACTGCTCTGAGTATGCATTTGTTTTCAATTCATCTTTATTTCAA TCTGTTTAACTTTTGAATCGCATGGGAATACGCACATTAAGTTCCTTTCTAAAATAAGGTTTTATGAAGC TGAGTTTCACGATAAGTGTCTTGCTATTTTTTGAGATGTTTTATGGACAAAGAAAACTTTACAGATTTAT ATGTATTTTGCTGCACCAGTAAATGGACCATTAACTAGGGCCCACCTTTAACAGAGCACCCCTTTGAAAG TTTTATAGGTATGAAATATATGTAGATATTTGTAAAGGGTTTTAATTTTTTTTTTTTGATGGGGTGCTGT GTAAATCTTGTATTTATAAATGTAATGAAGGTATTGACAGAAAAAAATATATACAACTTTTATAAAGGAT TGTGTACTGACTGAATACATTTAAAAGAAAATATATTTTGAAACCTGTTCTGCTATGAACAGAGATAACA TATCTTTTTACTATGCTATTGGTTTTTAGGTTAAGCTTCCTAATGCATAATAAATTTACAGTGGTTAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD140114 unedited TTGCGTGGGGCCTGTTGACAACTCCAATGTTGACTGTTCTACATGAAACATTTTCTCAAC ACACATTCCTCATGAATGGTCTCATTCAAGGTGTAAAGGGCCTGCTCTCTTTTTTGAGTG CCCCACTCATTGGTGCCCTGTCTGATGTGTGGGGGAGGAAGCCCTTTCTCCTCGGCACTG TATTCTTTACCTGCTTCCCAATCCCACTGATGAGGATCAGCCCATGGTGGTATTTTGCGA TGATTTCTGTGTCTGGAGTCTTCTCGGTCACGTTTTCTGTTATATTTGCCTATGTAGCTG ATGTCACTCAGGAGCACGAGCGAAGTACAGCTTATGGATGGGTCTCAACCACCTTTGCGG CTAGTCTTGTCAGCAGCCCGGCCATTGGAGCATATCTTTCTGCCAGCTACGGAGACAGCC TCGCTGTGCTGGTGGCCACAGTGGTGGCTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD140114 unedited GGGTGGGGCCCCCCCNGTTACATTTCANNNNNNAGCNNTTGGTGGATATATAAACGCTNT TGTAAGCTTAACCTAAAAACCATAGCATAGTAAAAAGAATGTTATCTCTGTTCATAGCAG AACAGGTTTCAAAATATATTTTCTTTTAAATGTATTCAGTCAGTACACAATCCTTTATAA AAGTTGTATATATTTTTTTCTGTCAATACCTTCATTACATTTATAAATACAAGATTTACA CAGCACCCCATCAAAAATAAAAAATTAAAACCCTTTACAAATATCTACATATATTTCATA CCTATAAAACTTTCAAAGGGGTGCTCTGTTAAAGGTGGGCCCTAGTTAATGGTCCATTTA CTGGTGCAGCAAACTACATATAAATCTGCAAAGCCTTCTTTGTCCATAAAACATCTCAAA AAATAGCAAGACACTTATCGTGACAACTCAGCTTCATAAAACCTCATTTTATAAAGGAAC TCAATGTGCGCATTCCCTCGTTATTCACACGTCAAACAGATTGAACTTACCACTAATCGA AAACCCACGCCTCCTTATCACTTCCTTTGCCCCACTGTGATGCATCATACAACCTTTTTT ATTTACGACATCGCACCACCTCCTATTTACCCCCTTTATACTTCCCCCCCTTCGTCCCCT CCCCTTTGTCTTTTTTCTTTTTCTCGCCTCCCCCCCTCTACCTCCTCTTCCTCTCCCCCG TTCCCTTTTCTCCCCCCACATTTCTTTCCCCACACCATACCATCTCCCCTTTTTCCCCCA CGATTCTTCCCCTCCTCCTCCCCCTCCCCTTTTATCTCTCTGTGCTGTGTCTAGACCCCT TTTTCTCCCCCCCCCCCCTTTTCTTCCCTACCCTACCCCACCGTATGTTTCCCTCTCCTC TGTTTTTTCTCCACCCCCCCCTCCCGATTTTTTATTTCACCGCCCCCTCCTCTTTCACCG TTACTCACCCTTATCTACCTCCCCTCCCACCCCAG
Restriction Sites	NotI-NotI
ACCN	BD140114
Insert Size	3260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD140114.1
RefSeq Size	3184 bp
RefSeq ORF	3184 bp
Locus ID	84641
Protein Families	Transmembrane

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