ATG16L1 (BC013411) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APG16L; ATG16A; ATG16L; IBD10; WDR30
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC013411, the custom clone sequence may differ by one or more nucleotides AAGACCGTCCCGGATGGCCTCGGGGACGGCCAGTGTGTGGAGGTGAGCTCCGGGATTGCCGGCATTCCCG CTTCTGCTGGTTGCTTCATGCTGCAGGCTGCGGCCGTCAGCCCTCGCTCGCATTGGTGGCGCTGAGGTGC CGGGGCAGCAAGTGACATGTCGTCGGGCCTCCGCGCCGCTGACTTCCCCCGCTGGAAGCGCCACATCTCG GAGCAACTGAGGCGCCGGGACCGGCTGCAGAGACAGGCGTTCGAGGAGATCATCCTGCAGTATAACAAAT TGCTGGAAAAGTCAGATCTTCATTCAGTGTTGGCCCAGAAACTACAGGCTGAAAAGCATGACGTACCAAA CAGGCACGAGATAAGTCCCGGACATGATGGCACATGGAATGACAATCAGCTACAAGAAATGGCCCAACTG AGGATTAAGCACCAAGAGGAACTGACTGAATTACACAAGAAACGTGGGGAGCTCAACTGGTGATTGACCT GAATAACCAAATGCAGCGGAAGGACAGGGAGATGCAGATGAATGAAGCAAAAATTGCAGAATGTTTGCAG ACTATCTCTGACCTGGAGACGGAGTGCCTAGACCTGCGCACTAAGCTTTGTGACCTTGAAAGAGCCAACC AGACCCTGAAGGATGAATATGATGCCCTGCAGATCACTTTTACTGCCTTGGAGGGAAAACTGAGGAAAAC TACGGAAGAGAACCAGGAGCTGGTCACCAGATGGATGGCTGAGAAAGCCCAGGAAGCCAATCGGCTTAAT GCAGAGAATGAAAAAGACTCCAGGAGGCGGCAAGCCCGGCTGCAGAAAGAGCTTGCAGAAGCAGCAAAGG AACCTCTACCAGTCGAACAGGATGATGACATTGAGGTCATTGTGGATGAAACTTCTGATCACACAGAAGA GACCTCTCCTGTGCGAGCCATCAGCAGAGCAGCCACGAGACGCTCTGTCTCTTCCTTCCCAGTCCCCCAG GACAATGTGGATGCTCATCCTGGTTCTGGTAAAGAAGTGAGGGTACCAGCTACTGCCTTGTGTGTCTTCG ATGCACATGATGGGGAAGTCAACGCTGTGCAGTTCAGTCCAGGTTCCCGGTTACTGGCCACTGGAGGCAT GGACCGCAGGGTTAAGCTTTGGGAAGTATTTGGAGAAAAATGTGAGTTCAAGGGTTCCCTATCTGGCAGT AATGCAGGAATTACAAGCATTGAATTTGATAGTGCTGGATCTTACCTCTTAGCAGCTTCAAATGATTTTG CAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACACTCACGGGACACAGTGGGAAAGTGCT GTCTGCTAAGTTCCTGCTGGACAATGCGCGGATTGTCTCAGGAAGTCACGACCGGACTCTCAAACTCTGG GATCTACGCAGCAAAGTCTGCATAAAGACAGTGTTTGCAGGATCCAGTTGCAATGATATTGTCTGCACAG AGCAATGTGTAATGAGTGGACATTTTGACAAGAAAATTCGTTTCTGGGACATTCGATCAGAGAGCATAGT TCGAGAGATGGAGCTGTTGGGAAAGATTACTGCCCTGGACTTAAACCCAGAAAGGACTGAGCTCCTGAGC TGCTCCCGTGATGACTTGCTAAAAGTTATTGATCTCCGAACAAATGCTATCAAGCAGACATTCAGTGCAC CTGGGTTCAAGTGCGGCTCTGACTGGACCAGAGTTGTCTTCAGCCCTGATGGCAGTTACGTGGCGGCAGG CTCTGCTGAGGGCTCTCTGTATATCTGGAGTGTGCTCACAGGGAAAGTGGAAAAGGTTCTTTCAAAGCAG CACAGCTCATCCATCAATGCGGTGGCGTGGTCGCCCTCTGGCTCGCACGTTGTCAGTGTGGACAAAGGAT GCAAAGCTGTGCTGTGGGCACAGTACTGACGGGGCTCTCAGGGCTGGGAGGACCCCAGTGCCCTCCTCAG AAGAAGCACATGGGCTCCTGCAGCCCTGTCCTGGCAGGTGATGTGCTGGGTATAGCATGGACCTCCCAGA GAAGCTCAAGCTATGTGGCACTGTAGCTTTGCCGTGAATGGGATTTCTGAAGATTTGACTGAGGTCTCTC TTGGCCTGGAAGAATAACACTGAAAAAAACCTGACGCTGCGGTCACTTAGCAGAGGCTCAGGTTCTTGCC TTGGGAAACACTACTAGCTCTGACCTTCCATACCTCACTTGGGGGAGCACAGGGCCCCGCTGGGCCTCCT CACCAACGGCAGTGCCAAAATCAGCCCCCACATCAAGGTGGTGTTCTCTGTGCTTTCTCTCGTCCTTCCA AAGTCGGTTCTGGCCTAACGCATGTCCCAACACCTTGGGTTCATTTGCCCGGTGAACTCACTTTAAGCAT TGGATTAACGGAAACTCCCGAACTACAGACCCCTCCCTGGTGGGTTGCATGAATGTGTCTCATTACTGCT GAAATGTCCTCACATCTCTTCCACTGTTCTTCAGAGCTTTCTGGCTCTCTTTCCCCACAAAATTCGACAC ATTTAAAAATCTCCGTGTGGCTTTAAAAAATGGTTTTTTGTTTTTTTGTTTTTTTGAGGTGGGAGAGGAT GTGTGAAAATCTTTTCCAGGGAAATGGGTTCGCTGCAGAGGTAAGGATGTGTTCCTGTATCGATCTGCAG ACACCCAGAAGGTGGGTGCACACTGCATGCTTGGGGGTGCCAAGGGATTCGAGACCTCCAACATACTTGT CTGAAGGTGGTGATTCTGGCCATGGCCCCTCTGCCAAGCCTGTGTGCGATGCCCTTGGTGCTTTAGTGCA AGAAGCCTAGGCTCAGAAGCACAGCAGCGCCATCTTTCCGTTTCAGGGGTTGTGATGAAGGCCAAGGAAA ACATTTATCTTTACTATTTTACCTACGTATAAAGTTTTAGTTCATTGGGTGTGCGAAACACCCTTTTTAT CACTTTTAAATTTGCACTTTATTTTTTTTCTTCCATGCTTGTTCTCTGGACATTTGGGGATGTGAGTGTT AGAGCTGGTGAGAGAGGAGTCAGGCGGCCTTCCCACCGATGGTCCTGGCCTCCACCTGCCCTCTCTTCCC TGCCTGATCACCGCTTTCCAATTTGCCCTTCAGAGAACTTAAGTCAAGGAGAGTTGAAATTCACAGGCCA GGGCACATCTTTTATTTATTTCATTATGTTGGCCAACAGAACTTGATTGTAAATAATAATAAAGAAATCT GTTATATACTTTTCAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC013411 unedited TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCG GCACGAGGGCCTCGGGGACTGCCAGTGTGTGGAGGTGAGCTCCGGGATTGCCGGCATTCC CGCTTCTGCTGGTTGCTTCATGCTGCAGGCTGCGGCCGTCAGCCCTCGCTCGCATTGGTG GCGCTGAGGTGCCGGGGCAGCAAGTGACATGTCGTCGGGCCTCCGCGCCGCTGACTTCCC CCGCTGGAAGCGCCACATCTCGGAGCAACTGAGGCGCCGGGACCGGCTGCAGAGACAGGC GTTCGAGGAGATCATCCTGCAGTATAACAAATTGCTGGAAAAGTCAGATCTTCATTCAGT GTTGGCCCAGAAACTACAGGCTGAAAAGCATGACGTACCAAACAGGCACGAGATAAGGAG GCGGCAAGCCCGGCTGCAGAAAGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCAGTCGA ACAGGATGATGACATTGAGGTCATTGTGGATGAAACTTCTGATCACACAGAAGAGACCTC TCCTGTGCGAGCCATCAGCAGAGCAGCCACTAAGCGACTCTCGCAGCCTGCTGGAGGCCT TCTGGATTCTATCACTAATATCTTTGGGAGACGCTCTGTCTCTTCCTTCCCAGTCCCCCA GGACAATGTGGATACTCATCCTGGTTCTGGTAAAGAAGTGAGGGTACCAGCTACTGCCTT GTGTGTCTTCGATGCACATGATGGNGAAGTCAACGCTGTGCAGTTCAGTCCAGGTTCCCG GTTACTGGCCACTGGNAGCATGGACCGCAGGNTTAAGCTTGGGAAAGTATTGAGAAAAAG TGAGTTCAAGGGTCCTATCTGGCAGTATGCAGAATACAGCATGATTTGAAGTGCTGGATC TACTNCTACAGCTCAAGAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC013411 unedited CACTTCAGGATCGAGTTTTTTTCCTTTTTTTTTTTTTTTTTTTGAAAAGTTATAACAGAT TTCTTTATTATTATTTACAATCAAGTTCTGTTGGCCAACATAATGAAATAAATAAAAGAT GTGCCCTGGCCTGTGAATTTCAACTCTCCTTGACTTAAGTTCTCTGAAGGGCAAATTGGA AAGCGGTGATCAGGCAGGGAAAAAAGGGCAGGTGGAGGCCAGGACCATCGGTGGGAAGGC CACCTGACTCCTCTCTCACCAGCTCTAACACTCACATCCCCAAATGTCCAGAGAACAAGC ATGGAAGAAAAAAAATAAAGTGCAAATTTAAAAGTGATAAAAAGGGTGTTTCGCACACCC AATGAACTAAAACTTTATACGTAGGTAAAATAGTAAAGATAAATGTTTTTCCTTGGCCTT CATCACAACCCCTGAAACGGAAAGATGGCGCTGCTGTGCTTCTGAGCCTAGGCTTCTTGC ACTAAAGCACCAAGGGCATCGCACACAGGCTTGGCAGAGGGGCCATGGCCAGAATCACCA CCTTCAGACAAGTATGTTGGAGGTCTCGAATCCCTTGGCACCCCCAACATGCAGTGTGCA CCCCCCTTCTGGGTGTCTGCAGATCGATACAGGAACACATCCCTACCTCTGGAGCGAACC AATTTCCCTGGGAAAGATTTTCACACATTCTCTCCCACCTTAAAAAAACAAAAAACAAAA ACCCTTTTTTAAAGCCCCACGGGAGATTTCTAAAATTGCCCAAATTCTGTGGGGGAAGGA GACGCCATAAGCCCTTGAAAACAAGTGAAGAGATTGTGAGGCCTTTCACGCGTAATGAAA ACCCTTTTTGGCCCCCACCGGAAGGGCCTTTACTTGGGAATTCCCTTATCCAAGCTTAAG GGAGCCCCCGCCATAACCCAGGGTTGGAATGCTTTAGCAACACCATTTGGAGCCGAAAAC ACCCAAACCCCTTTTGTGGGGCTATTGCATGACT
Restriction Sites	NotI-NotI
ACCN	BC013411
Insert Size	2930 bp
OTI Disclaimer	The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
OTI Annotation	This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013411.1
RefSeq Size	3262 bp
RefSeq ORF	357 bp
Locus ID	55054
Gene Summary	The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.