ATP5D (AK094357) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK094357, the custom clone sequence may differ by one or more nucleotides GTCCTCCTCGCCCTCCAGGCCGCCCGCGCCGCGCCGGAGTCCGCTGTCCGCCAGCTACCCGCTTCCTGCC GCCCGCCGCTGCCATGCTGCCCGCCGCGCTGCTCCGCCGCCCGGGACTTGGCCGCCTCGTCCGCCACGCC CGTGCCTATGCCGAGGCCGCCGCCGCCCCGGCTGCCGCCTCTGGCCCCAACCAGATGTCCTTCACCTTCG CCTCTCCCACGCAGGTTCGGGCGCTGCGGGTCGGGACCCTCCGTGGCCGCCGCCCCCGGAGTCCAGGGTC CCCACCCCCAGGGCGCGACCCCCGCTTCCGGGCCCCCGGACCCGCGCGCCCCGGAAGCCGAGGCTACTCA GCCCTGGACCCTCGGCCCTGCCTCTGCTGCCCGAGCCCTGCGCTGCCCTCGTCCCGGGCACCTCCCCGAG ATAAGCGTCTCCTGGGTGCCCTCCCCGATCTCCCTGGCCAAAGCCTGGGTGTCGCCGGATCGTTGCATTC CCATTTCGCGGATCAGTAGAATGAGGCGCAACTGTTGAGGACCAAAGCTGCAACTTCGGATCCCTGCGCT GGGTTGTCTCAGTGCCTCACATCAGCGCCAGGTCCTGCCCTGACCCATTACTTAGCAGGACAGGCCGAGT GCCCGGTGGGCGCGGGGCCGGCCTGCCCCGCCATCATTCCCCACGCTGTTGTCTGCAGGTGTTCTTCAAC GGTGCCAACGTCCGGCAGGTGGACGTGCCCACGCTGACCGGAGCCTTCGGCATCCTGGCGGCCCACGTGC CCACGCTGCAGGTCCTGCGGCCGGGGCTGGTCGTGGTGCATGCAGAGGACGGCACCACCTCCAAATACTT TGGTGAGTCCGGTGGAGGGCTGCAGGGCCAGGCCAGGCTGGGGCTCCACATCCCAGGTCAGTCTCCGTCT CAGTTGAGGCTGCGAGAAAAAATAGTTTTAGGCCGGGCACGGTGGCTCAAGCCTGTAATCCCAGCACTTT GGGAGGCCGAGGCAGGTGGATCACCTGAGGTCAGAAGTTCGGAGACCAGCCTGGCCAACATGATGTAACC CCGTCTCTTCTAAAAATACAGAAAACTGGCCGGGTGCGGTGGCTCCCGCCTGTAATCCCAGCACTTTGGG AGGCCGAGGCGGGGGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCT CTACTAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGTGCCTGTAGTCCCAGCTAGTCAGGAGGCTG AGGAGATTGTGCCACTGCACTCCAGCCTGGGCAACAAGAGTGAAACTCCATCTCAAATAAGAAAAATACA GTTTTAGCAGGTTTGTCCACTGAGGAGCCTGGGCTGGCCGGTGATGAAAGCGAACCAGAGGGGACCCTGC TCTCAGCCGGTTTATGACTGGCTCTGGACCAACCAATGTAGGATGGTCGAAGCAAGAGATGTAAAACCAC CCAGGCCCAGTGGTTCATGCCTGTAATCTCAGCACTTTGGGAGACTGAAGTGGGAGAATCGCTTAAGCCC AGGAGTTTGAGACTAGCCTTGGCAACATAGTGAGACCCTGTCTCTAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094357 unedited GTATACGACTTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCCGCGCCGGAGTCCG CTGTCCGCCAGCTACCCGCTTCCTGCCGCCCGCCGCTGCCATGCTGCCCGCCGCGCTGCT CCGCCGCCCGGGACTTGGCCGCCTCGTCCGCCACGCCCGTGCCTATGCCGAGGCCGCCGC CGCCCCGGCTGCCGCCTCTGGCCCCAACCAGATGTCCTTCACCTTCGCCTCTCCCACGCA GGTGTTCTTCAACGGTGCCAACGTCCGGCAGGTGGACGTGCCCACGCTGACCGGAGCCTT CGGCATCCTGGCGGCCCACGTGCCCACGCTGCAGGTCCTGCGGCCGGGGCTGGTCGTGGT GCATGCAGAGGACGGCACCACCTCCAAATACTTTGTGAGCAGCGGTTCCATCGCAGTGAA CGCCGACTCTTCGGTGCAGTTGTTGGCCGAAGAGGCCGTGACGCTGGACATGTTGGACCT GGGGGCAGCCAAGGCAAACTTGGAGAAGGCCCAGGCGGAGCTGGTGGGGACAGCTGACGA GGCCACGCGGGCAGAGATCCAGATCCGAATCGAGGCCAACGAGGCCCTGGTGAAGGCCCT GGAGTAGGCGAGCCAGCCGCCAAGGTTGACCTCAGCTTCGGAGCCACCTCTGGATGAACT GCCCCCAGCCCCCGCCCCATTAAAGACCCGGAAGCCTGAAAAAAAAACAAACNAAACANC CCCNCCACCCCCCCNTCTCCCCCCACCCCAAAAAAACAAAAAAACNCCCTGACCTTAAAT GCGCCGCGGCATACTGTTTCTGAACAACCCGGTGAATCCTGGACCCTCCAGGGCCTTCTG CCTGGATTGCCCTCAGGCCCCCACTGCCTATAATTAGTGCTTCTTGCGACAGGCCTCTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094357 unedited GGGCATGGGGTCTTTCAGGGGTGGGGGCTGGGGGCAGTTCATCCACAGTGGCTCCGAAGC TGATGTCAACCTTGGCGGCTGGCTCGCCTCTCCAGGCCTTCACCAGGGCCTCGTTGGCCT CGATTCGGATCTGGATCTCTGCCCGCGTGGCCTCGTCAGCTGTCCCCACCAGCTCCGCCT GGGCCTTCTCCAAGTTTGCCTTGGCTGCCCCCAGGTCCAACATGTGCAGCGTCACGGCCT CTTGGGCCAACAACTGCACCGAAGAGTCGGCGTTCACTGCGATGGAACCGCTGCTCACAA AGTATTTGGAGGTGGTGCCGTTCTCTGCATGCACCACGACCAGCCCCGGCCGCAGGACCT GCAGCGTGGGCACGTGGGCCGCCAGGATGCCGAAGGCTCCGGTCAGCGTGGGCACGTCCA CCTGCCGGACGTTGGCACCGTTGAAGAACACCTGCGTGGGAGAGGCGAAGGCGAAGGACA TCCGGCTGGGGCCAGAGGCGGCAACGCGGGGCGGTGGCCGCCTCGGTATACGCACCGTCG TGGCGGACGATGCGGCCAATTCCCGGGCGGTGGACCCTGCGGCCGGCACCCTGCCCCGGC GGGCGGCTCGAACCGGTTATCTGGCGGACAGCGGGATTTGGCGCGGGGCCCTGGTGCCGG AATTGGGGGGCGCCCCTTAGGGAGCCGTGTTCCCCAACTCCGCCCGTCCACCTAACCGCC GCTTGCCGCTTCCCCCCTCCTATCGCGCACTCCTACCGAGCCTTCGCCAGCCCGCGCCGG GCTTTTATCCCCCCTTGCCACCGCCTTTGCTTCGGTGCCCCAACAACCCCCCTCTGCCGT TATGGGGCCGGTACCTGGACATCCCTGCGGTCAACCCGTTACCCCCGCCTTGGGGTCTCC CCTAACCCCTTCACCAGGGATAACCGGGACTN
Restriction Sites	NotI-NotI
ACCN	AK094357
Insert Size	840 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK094357.1
RefSeq Size	1587 bp
RefSeq ORF	1587 bp
Locus ID	513
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
Gene Summary	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

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